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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

Product#: DCP-MEMH-QP1X
$49.50
DCP-MEMH-QP1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Without L-Glutamine Without Sodium Pyruvate

FluxMPS™ DCP-MEMH-QP1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for primary fibroblasts, neurons, epithelial and vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, while 25 mM HEPES (pKa 7.3 at 37°C) provides robust pH buffering independent of CO2. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Phenol Red | [-] L-Glutamine, [-] Sodium Pyruvate.

  • Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high-glucose media stress
  • NEAA pre-loaded — reduces de novo amino acid synthesis burden and ammonia accumulation in primary fibroblasts, neurons and epithelial cells
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO2-independent pH stability for open-bench handling, flow cytometry prep and atmospheric incubation
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish — mycoplasma-retentive purity for microfluidic and OoC platforms
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) — below the TLR4 activation threshold for sensitive primary and hematopoietic cultures
  • L-Glutamine and sodium pyruvate withheld for independent, user-defined supplementation and precise metabolic flux control
  • Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis
  • Custom pH, glucose and HEPES concentration formulations available on request
DCP-MEMH-QP1X | Size: 500 mL and 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM, pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Phenol Red
    [-] L-Glutamine, [-] Sodium Pyruvate
  • AppearanceOrange-to-red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290 — 330 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes mycoplasma, particulates, and endotoxin that alter primary cell phenotype. FluxMPS™ is Microfluidics Suitable, addressing these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate primary cell media prevents chip channel clogging.

biotech

Primary cell—optimized formulation

MEM + Earle's Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: CO2-stable pH

25 mM HEPES helps stabilize pH during open-air suspension culture handling, flow cytometry prep, and multi-well assay setup outside CO2 incubators.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin release specification — below the TLR4 activation threshold. For primary cells, this specification is relevant to avoiding LPS-driven fibroblast activation, neuroinflammatory signaling, and epithelial barrier disruption.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

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Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I — Large Particulate & Debris Removal

    Removes large aggregates and cell debris; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I — Mycoplasma-Retentive Barrier

    Retains mycoplasma (0.2–0.3 µm) and sub-micron particulates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II — Redundant Protection

    A second, dedicated prefilter protecting the second 0.04 µm cartridge, giving the train full pair redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size — mycoplasma-retentive polishing
Sterility & Mycoplasma: No growth after 14-day sterility incubation (USP <71>). Mycoplasma risk is mitigated by 0.04 µm mycoplasma-retentive filtration at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEMH-QP1X Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate quadruple-stage filtration system: 0.1 micron and 0.04 micron stages twice each, Microfluidics Suitable cell culture media for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-QP1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-QP1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant of this formulation is available on request for automated bioreactor and robotic handling systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically appropriate base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline supports confocal, TEER sensor, and biosensor applications on primary cell chips; a phenol red–free variant of this formulation is available on request for applications requiring reduced background fluorescence.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Phenol Red | [-] L-Glutamine, [-] Sodium Pyruvate
Appearance Orange-to-red colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 290 — 330 mOsm/kg H2O
Total ingredients 36 across 4 category groups (3 tabs)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100) aseptic fill
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 recommended for optimal bicarbonate-mediated pH control; 25 mM HEPES provides supplemental, CO2-independent buffering for extended bench-top handling
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Pack sizes 500 mL, 1000 mL
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 36 ingredients across 4 category groups (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 browsable tabs, verified per lot with CAS numbers where available.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 11.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-QP1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016–certified quality management system. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — minimizes trace-metal and organic (TOC) contaminants.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm); light obscuration.

Osmolality — USP <785>

Target: 290 — 330 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-MEMH-QP1X lot at support@diagnocine.com.
Product Comparison

How DCP-MEMH-QP1X compares

FluxMPS™ DCP-MEMH-QP1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH-QP1X (FluxMPS™) Conventional MEM Low Glucose (0.22 µm filtered) Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Standard grade Standard grade
MEM + NEAA + 25mM HEPES — no L-Glutamine, no Pyruvate; dual metabolic control with stable pH check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.04 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Ultrapure Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Yes Limited cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-QP1X.

DCP-MEMH-QP1X is Microfluidics Suitable, processed through a quadruple-stage filtration system reaching 0.04 µm, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with mycoplasma-retentive filtration purity is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.
 
Both are omitted for independent fresh supplementation at user-defined concentrations. HEPES + sodium bicarbonate together provide dual-buffer pH stability throughout. This enables precise nitrogen (glutamine) and secondary carbon source (pyruvate) control for primary cell metabolic flux studies, with stable pH during sampling events outside the incubator.
5% CO2 is recommended for optimal bicarbonate-mediated pH control. The 25 mM HEPES component provides supplemental, CO2-independent buffering for extended bench-top handling outside the incubator.
Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane prior to addition — a 0.04 µm membrane will strip serum of the very lipoproteins and growth factors that make it functional and is not recommended for this purpose. Defined, protein-free additions may use a 0.1 µm membrane.
Every DCP-MEMH-QP1X batch is released against a specification of < 0.05 EU/mL, tested by LAL assay per USP <85> (assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch, not per unit. For primary cells, endotoxin can activate TLR4, altering fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432—437.doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662—1668.doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760—772.doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407—420.doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117—129.doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119—1129.doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588—3598.doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446—452.doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201—1212.doi:10.1039/c3lc41017j

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