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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES: 1X Liquid

Product#: DCP-MEMH1X
$49.50
DCP-MEMH1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate

FluxMPS™ DCP-MEMH1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Minimum Essential Medium (MEM) formulated with Earle's Salts, Low Glucose (1.0 g/L), non-essential amino acids (NEAA), and 25 mM HEPES, engineered for primary fibroblasts, neurons, epithelial, and vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides robust pH stability during handling outside CO2 incubators.

  • Low Glucose (1.0 g/L) — a physiological carbon source for primary cells sensitive to high-glucose media
  • Non-essential amino acids (NEAA) pre-loaded — reduces metabolic burden and ammonia accumulation from de novo synthesis
  • 25 mM HEPES (pKa 7.3 at 37°C) alongside 2200 mg/L sodium bicarbonate for dual-buffer pH stability during open-air handling
  • Quadruple-stage filtration to a 0.04 µm final pore size: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm
  • Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>) for endotoxin-sensitive primary and hematopoietic cell cultures
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Earle's Salts base formulation supports fibroblast, neuronal, epithelial, and vascular primary cell models
  • Custom pH, glucose concentration, and HEPES level available on request — contact support@diagnocine.com
DCP-MEMH1X Cell Culture Media Sizes: 500 mL, 1000 mL
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • AppearanceOrange-to-red colored, clear solution
  • pH (USP <791>)7.4
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2-8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a mainstay of primary cell biology — but conventional 0.22 µm filtered MEM passes mycoplasma, subvisible particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ is built to reduce these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> particulate compliance. Low-particulate media helps prevent chip channel clogging in sub-100 µm microfluidic geometries.

biotech

Primary cell-optimized formulation

MEM + Earle's Salts + NEAA is a long-established base for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: buffering outside the incubator

25 mM HEPES (pKa 7.3 at 37°C) helps limit the pH rise that occurs when cultures are handled outside CO2 incubators — useful during imaging sessions, perfusion circuit changes, and multi-well assay setup.

shield

Low-endotoxin release specification

< 0.05 EU/mL endotoxin release specification (LAL, USP <85>). Low-endotoxin formulations are widely used for endotoxin-sensitive primary cell types — including fibroblasts, neurons, and epithelial cells — where LPS-driven TLR4 activation can confound experimental readouts.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation from amino acid synthesis pathways.

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Customization on demand

pH, glucose, HEPES concentration, and other component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish. The train is a repeated prefilter-plus-final-filter pair, run twice in series: each 0.04 µm final filter has its own dedicated 0.1 µm prefilter.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; a mycoplasma-retentive pore size (mycoplasma range 0.2–0.3 µm) not achieved by standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge from breakthrough.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish under validated ISO Class 5 conditions.

Performance vs. conventional media

FluxMPS™ DCP-MEMH1X is processed through a four-pass, two-pair filtration train reaching a 0.04 µm final pore size, well below the 0.22 µm industry standard.

0.04
µm final pore size
4
Filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility: No growth after 14-day incubation (USP <71>). Mycoplasma: controlled by 0.1 µm/0.04 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEMH1X Minimum Essential Medium (MEM), Low Glucose, NEAA and 25mM HEPES 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable cell culture medium for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH1X is purpose-built for primary fibroblasts, neurons, epithelial, and vascular cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic liquid-handling systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically appropriate base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s Salts ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline supports confocal, TEER sensor, and biosensor applications on primary cell chips. This formulation contains phenol red (11 mg/L); a phenol-red-free variant is available on request for fluorescence-sensitive assays.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com. Available sizes: 500 mL, 1000 mL.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate
Media family MEM Low Glucose + Earle's Salts + NEAA + 25mM HEPES
Appearance Orange-to-red colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 38 across 4 groups (Inorganic Salts, Amino Acids, Vitamins, Others)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 recommended (dual HEPES + bicarbonate buffering); HEPES independently maintains pH stability during CO2-free handling
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 38 ingredients across 4 groups, released per lot with CAS numbers where known. NEAA is listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 11.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

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ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm; trace-metal and organic-carbon (TOC) controlled for a consistent formulation background.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; a Certificate of Analysis is issued for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-MEMH1X lot at support@diagnocine.com.
Product Comparison

How DCP-MEMH1X compares

FluxMPS™ DCP-MEMH1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM (0.22 µm)
Grade Microfluidics Suitable (0.04 µm) Standard filtration (0.22 µm) Standard filtration (0.22 µm)
Full-formulation MEM + NEAA + 25mM HEPES — dual-buffered for pH stability across variable CO2 primary-cell OoC environments check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.04 µm) cancel No cancel No
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel Rarely cancel Rarely

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH1X.

Yes. DCP-MEMH1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels suited to MPS, OoC, and LoC platforms. The MEM + Earle's Salts + NEAA formulation, with mycoplasma-retentive filtration, is well suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.
FluxMPS™ uses four sequential filtration passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final pore size, well below the 0.22 µm used in conventional media, with mycoplasma-retentive filtration at every production stage.
Low Glucose (1.0 g/L) approximates physiological glucose levels for primary cells that are sensitive to the supraphysiological glucose found in high-glucose formulations, and pre-loaded NEAA reduces the synthesis burden on cells that would otherwise need to produce these amino acids de novo. If your protocol calls for a different glucose level, contact support@diagnocine.com for a custom formulation rather than supplementing glucose directly, which can shift osmolality.
5% CO2 is recommended for this dual HEPES + sodium bicarbonate–buffered formulation. The 25 mM HEPES component (pKa 7.3 at 37°C) independently helps maintain pH during brief handling outside a CO2 incubator — for example during imaging sessions, perfusion circuit changes, or multi-well assay setup — but is not intended to replace CO2 incubation for routine culture.
Yes. When adding serum or other protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane rather than a smaller pore size — a 0.04 µm membrane will retain much of the protein and lipoprotein fraction of serum. Defined, protein-free additions may be filtered at 0.1 µm. Contact support@diagnocine.com for supplementation guidance specific to your cell type.
DCP-MEMH1X is produced to a release specification of < 0.05 EU/mL by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification before it ships. For primary cells, minimizing endotoxin is relevant because LPS can activate TLR4 signaling, which may affect fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell and OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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