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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Product#: DCP-MEMH-QRN1X
$44.00
DCP-MEMH-QRN1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage ultra-filtration: 0.1 µm (Prefiltration I) → 0.04 µm (Final filtration I) → 0.1 µm (Prefiltration II) → 0.04 µm (Final filtration II — Polish)
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> BET), tested per manufacturing batch
  • Low-glucose MEM base (1000 mg/L) with 25 mM HEPES buffering; formulated without L-glutamine, sodium bicarbonate, or phenol red
  • HEPES-buffered, bicarbonate-free chemistry gives reduced CO₂ dependence and compatibility with Agilent Seahorse XF metabolic flux assays
  • Sodium pyruvate (110 mg/L) supplied as an alternate carbon/energy source
  • Manufactured under an ISO 13485:2016 quality management system, with aseptic fill in an ISO Class 5 (Class 100) environment
  • pH 7.4 (USP <791>); prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
  • Custom pH, glucose, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com
DCP-MEMH-QRN1X |Pack Sizes: 500 mL, 1000 mL |Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L)
  • L-GlutamineNot added — supplement as needed
  • Sodium BicarbonateNot added — bicarbonate-free, HEPES-buffered
  • HEPES5958 mg/L (25 mM)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See Certificate of Analysis
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • FiltrationQuadruple-stage (0.1 µm ×2 + 0.04 µm ×2)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is engineered to address these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particulates to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per batch.

target

Total metabolic control

Defined sodium pyruvate (110 mg/L) and a low-glucose (1.0 g/L) base support precise metabolic flux and Warburg-pathway experiments.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696) for low trace-metal and organic-carbon background.

visibility

Low background for imaging

Quadruple-stage filtration keeps the particulate baseline low, supporting confocal microscopy, live-cell biosensors, and TEER measurements.

science

Rich, stable nutrient profile

Micro-batch precision locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is processed through a four-stage serial filtration sequence — two dedicated prefilter/final-filter pairs — reaching a 0.04 µm final cut-off, addressing mycoplasma-sized and subvisible particulates that 0.22 µm filtration cannot retain.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    Fine particulate and mycoplasma-retentive filtration (0.2–0.3 µm organisms retained) beyond the reach of standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm final filter cartridge; redundancy against upstream breakthrough.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in an ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

Four sequential stages reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every batch.

5×
 
4
Sequential filtration passes to a 0.04 µm final cut-off
Sterility assurance: Every batch undergoes 14-day USP <71> sterility testing. Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration; lots are not individually tested for mycoplasma unless otherwise specified.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid (DCP-MEMH-QRN1X) Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II ? Polish, for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-QRN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is suited to organ-on-a-chip, metabolic flux, live-cell imaging, and primary cell applications where particulate load and endotoxin variability must be minimized.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion beyond this Microfluidics Suitable formulation.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade formulation.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation reduces microchannel clogging risk and supports laminar flow integrity.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined low-glucose carbon source and low-endotoxin background support precise metabolic flux analysis.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin can cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER stability in long-term culture.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

Bicarbonate-free, phenol red-free chemistry supports isotope tracing workflows and is compatible with Agilent Seahorse XF extracellular flux assays, which require bicarbonate-free, phenol red-free medium.

13C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate baseline from quadruple-stage filtration supports confocal imaging and biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Batch-release quality parameters

Every production batch of FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid undergoes the complete quality-release battery below before shipment.

Available pack sizes: 500 mL, 1000 mL

Physical & Chemical Parameters
Parameter Specification
Formulation Low-glucose MEM, 25 mM HEPES; without L-glutamine, sodium bicarbonate, or phenol red
Appearance Colorless to very pale yellow, clear solution (phenol red-free)
pH USP <791> 7.4
Osmolality USP <785> See Certificate of Analysis
Glucose 1000 mg/L (1.0 g/L)
L-Glutamine Not added — supplement as needed
Sodium Pyruvate 110 mg/L
Phenol Red Not added
Sodium Bicarbonate Not added — bicarbonate-free, HEPES-buffered
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696)
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement HEPES-buffered; reduced CO₂ dependence (validate per cell line)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full batch documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers. Total: 28 components across 4 categories. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Sodium Pyruvate 113-24-6 110.000
i-Inositol 87-89-8 2.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system, with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production batch.

filter_alt

Quadruple-Stage Filtration

Validated four-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off on every batch.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing ensures lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay on every manufacturing batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per batch.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: See Certificate of Analysis.

Documentation — CoA & Full Batch Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every batch.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com.
Product Comparison

How DCP-MEMH-QRN1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of a similar base formulation.

Parameter DCP-MEMH-QRN1X (FluxMPS™) Conventional MEM (0.22 µm) Standard MEM alternative
Grade Microfluidics Suitable (0.04 µm final cut-off) Not applicable Not applicable
Base Formulation Low-glucose MEM, 25 mM HEPES; w/o L-glutamine, sodium bicarbonate, phenol red MEM Standard MEM Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEMH-QRN1X uses our quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), delivering low particulate counts that reduce microchannel clogging risk in OoC and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or subvisible particulates that accumulate in microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1.
L-glutamine is unstable in liquid formulations and degrades over storage, so it is left out and added fresh (or as a stable dipeptide such as GlutaMAX) at time of use. Sodium bicarbonate is omitted because this formulation relies on 25 mM HEPES for pH buffering instead of a bicarbonate/CO₂ system. Contact support@diagnocine.com for custom concentrations or a bicarbonate-buffered variant.
This formulation is HEPES-buffered and bicarbonate-free, giving reduced CO₂ dependence. It can be used in ambient-air or low-CO₂ culture systems; validate against your specific cell line and application before switching from a CO₂/bicarbonate-buffered protocol.
Yes. This medium can be supplemented with FBS (5–20%), growth factors, L-glutamine, antibiotics, or other additives per standard practice. Serum and other protein-containing additions should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; defined, protein-free additions may use a 0.1 µm filter. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay per USP <85> BET (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before shipment. Batch-specific results are recorded on the Certificate of Analysis, available from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  5. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  6. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  7. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  8. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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