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FluxMPS™ Medium 199, Earle's Salts: 2X Liquid

Product#: DCP-M1992X
$90.00
DCP-M1992X
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FluxMPS™ Medium 199, Earle's Salts: 2X Liquid

MPS-grade Medium 199 at double (2X) concentration with Earle's salts, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system for organ-on-a-chip (OoC), tissue-chip and microfluidic perfusion work. With a Earle's bicarbonate buffer, it is microchannel-safe and approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.

  • Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
  • 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
  • Endotoxin < 0.05 EU/mL (USP <85> BET)
  • Formulation: [+] Earle's Salts, [+] 2 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red; [−] HEPES
  • Classic Morgan, Morton & Parker (1950) Medium 199 recipe with nucleic-acid precursors, cholesterol and fat-soluble vitamins
  • Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill
  • 2X concentrate: every component at double strength; dilute 1:1 to 1X working strength before use
  • pH, concentration, component additions and nutrient modifications available on request
DCP-M1992X|UNSPSC 41116155Cell culture media
Catalog No. DCP-M1992X · Sizes: 500 mL, 1000 mL
Medium 199, Earle's Salts: 2X Liquid
  • Glucose2,000 mg/L
  • L-Glutamine200 mg/L
  • Sodium Pyruvate0.220 mg/L
  • BufferNaHCO₃ 4,400 mg/L
  • pH7.4 (± 0.04)
  • Endotoxin< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8 °C, protect from light
  • Shelf Life12 months
  • Format2X liquid, 500 / 1000 mL
ISO 13485:2016USP <85> <785> <788>RUO
Why FluxMPS™

Engineered where standard media fails

Conventional Medium 199 is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and valves, and add noise to optical and electrical readouts in microphysiological systems (MPS).[3,11] FluxMPS™ Medium 199 keeps the original Earle's formulation and removes that failure mode.

filter_alt

Microchannel-safe purity

A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing and porous membranes.

target

Total metabolic control

Defined 2 g/L glucose, 200 mg/L L-glutamine and a trace 0.220 mg/L sodium pyruvate give a known carbon-source baseline for metabolic studies.

water_drop

Ultrapure-grade water

Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.

visibility

Low background for imaging

Particle-depleted medium reduces scatter and debris artifacts in confocal imaging and on-chip biosensors.[10]

science

Rich, stable nutrient profile

62 defined components, including nucleic-acid precursors, cholesterol, Polysorbate 80 and fat-soluble vitamins A, D, E and K, produced in controlled micro-batches.

tune

Customization on demand

pH, concentration, component additions and nutrient modifications (glucose, salts, HEPES) are available — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Every lot of DCP-M1992X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-use Medium 199 polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.

  1. 01

    0.1 µmPre-filtration I

    Removes large particulates and aggregates and extends the life of the downstream filters.

  2. 02

    0.04 µmPre-filtration II

    Retains fine particulates, bacteria and mycoplasma.

  3. 03

    0.1 µmSterile-filtration I

    Second-pass redundancy that catches anything shed or bypassed upstream.

  4. 04

    0.04 µmSterile-filtration II — Final Polish

    Final nano-polish immediately before ISO Class 5 aseptic fill.

Performance vs. conventional media

By particulate count, FluxMPS™ Medium 199 is approximately 5× cleaner than conventional 0.22 µm-filtered Medium 199, lowering clog risk in narrow channels and long perfusion runs.

5×
cleaner by particulate count vs. 0.22 µm media
0.04
µm final filtration pore size
Sterility & mycoplasma assurance: no bacterial or fungal growth is observed after 14 days of incubation, as per USP specification (USP <71>), and the 0.04 µm stages provide USP <63>-equivalent mycoplasma assurance.[8]
FluxMPS™ Medium 199, Earle's Salts: 2X Liquid (DCP-M1992X) Quadruple-stage filtration system diagram: 0.1 μm pre-filtration, 0.04 μm pre-filtration, 0.1 μm sterile filtration and 0.04 μm final polish for MPS-grade Medium 199 cell culture media used in organ-on-a-chip and microfluidic applications, Diagnocine
Figure 1. Quadruple-stage filtration: 0.1 µm pre-filtration, 0.04 µm pre-filtration, 0.1 µm sterile filtration and 0.04 µm final polish before ISO Class 5 fill.
© Diagnocine® — DCP-M1992X
Applications

Where FluxMPS™ Medium 199 fits

Medium 199 was the first nutritionally defined medium, developed by Morgan, Morton and Parker in 1950 for nutritional studies on primary chick embryo fibroblasts in the absence of any additives.[1] Explanted tissue could survive in it without serum, although long-term cultivation of cells required serum supplementation. Medium 199 is formulated with either Hank's salts or Earle's salts; this product uses Earle's salts.[2] Supplemented with serum, it supports growth of a wide variety of cells, and it is presently used for the maintenance of non-transformed cells, vaccine and virus production, and primary explants of epithelial cells.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.

  • Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
  • Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
  • Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.

Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.

Microfluidics

Micro Physiological System (MPS) & Chip

Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms and lab-on-a-chip assays.[3,4,5,6,7]

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

A defined glucose baseline (1 g/L at 1X working strength) for glycolysis and metabolic-shift studies in tumor lines, alone or on chip.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Base medium for maintenance of differentiated iPSC-derived cells in perfused chip models, with serum or defined supplements.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Medium 199 is the classic base for human umbilical vein endothelial cell culture and for non-transformed primary cells and epithelial explants.[12]

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Known, low-particulate composition simplifies isotope tracing, respirometry and NMR sample prep.

¹³C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Fewer particles means cleaner fields for confocal imaging, on-chip biosensors and barrier readouts.[10]

ConfocalBiosensorsTEER
Technical Specifications

Specifications & quality control

Release specifications for DCP-M1992X. Lot-specific values are reported on the Certificate of Analysis.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] 2 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red; [−] HEPES
Appearance Red
pH 7.4 (± 0.04) USP <791>
Osmolality Measured per lot; reported on CoA USP <785>
Glucose 2,000 mg/L (2 g/L)
L-Glutamine 200 mg/L
Sodium Pyruvate 0.220 mg/L
Sodium Bicarbonate 4,400 mg/L
HEPES Not included
Phenol Red 30 mg/L
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin < 0.05 EU/mL USP <85> BET
Sterility No bacterial or fungal growth after 14 days of incubation USP <71>
Mycoplasma 0.04 µm barrier filtration USP <63> equiv.
Particulate ≥10 µm Meets limits USP <788> M2
Particulate ≥25 µm Meets limits USP <788> M2
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100) ISO
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8 °C, away from bright light
Freeze-thaw Do not freeze
Shelf life 12 months; use before the expiry date on the product label
Shipping condition Ice Pack
CO₂ requirement 5% CO₂ (Earle's salts with 4.4 g/L sodium bicarbonate (2.2 g/L after 1:1 dilution to 1X))
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade High-purity, cell-culture-tested components
Traceability Lot-traceable; CoA per lot
Manufacturing QMS ISO 13485:2016 ISO
Regulatory alignment 21 CFR Part 820 (cGMP) aligned; ISO 13485-certified and CE-approved facilities
Production method Micro-batch 2X liquid, quadruple-stage filtered
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

This product is Medium 199 at a 2X concentration with Earle's salts, L-glutamine, sodium pyruvate, sodium bicarbonate and Phenol Red. It does not contain HEPES. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 62 components are listed below; values are per-lot release basis. This 2X concentrate contains every component at twice the 1X level; dilute 1:1 (for example with sterile water, a supplement solution, or an agar overlay) to reach 1X working strength before use.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 530.000
Ferric nitrate nonahydrate 7782-61-8 1.440
Magnesium sulphate anhydrous 7487-88-9 195.44
Potassium chloride 7447-40-7 800.000
Sodium acetate anhydrous 127-09-3 100.000
Sodium bicarbonate 144-55-8 4400.000
Sodium chloride 7647-14-5 13600.00
Sodium phosphate monobasic 7558-80-7 244.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 100.000
L-Alanine 56-41-7 50.000
L-Arginine hydrochloride 1119-34-2 140.000
L-Aspartic acid 56-84-8 60.000
L-Cysteine hydrochloride monohydrate 7048-04-6 0.200
L-Cystine dihydrochloride 30925-07-6 52.000
L-Glutamic Acid 56-86-0 134.000
L-Glutamine 56-85-9 200.000
L-Histidine hydrochloride monohydrate 5934-29-2 44.000
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 40.000
L-Leucine 61-90-5 120.000
L-Lysine hydrochloride 657-27-2 140.00
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 50.000
L-Proline 147-85-3 80.000
L-Serine 56-45-1 50.000
L-Threonine 72-19-5 60.000
L-Tryptophan 73-22-3 20.000
L-Tyrosine disodium salt 69847-45-6 115.32
L-Valine 72-18-4 50.000
Component CAS Number mg/L
VITAMINS
Ascorbic acid 50-81-7 0.10
Calciferol 50-14-6 0.200
Choline chloride 67-48-1 1.000
D-Biotin 58-85-5 0.020
D-Ca-Pantothenate 137-08-6 0.020
DL-Tocopherol phosphate Disodium Salt 60934-46 0.020
Folic acid 59-30-3 0.020
Menadione 58-27-5 0.020
Nicotinamide 98-92-0 0.050
Nicotinic acid 59-67-6 0.050
Pyridoxal hydrochloride 65-22-5 0.050
Pyridoxine hydrochloride 58-56-0 0.050
Retinol Acetate 127-47-9 0.280
Riboflavin 83-88-5 0.020
Thiamine hydrochloride 67-03-8 0.020
i-Inositol 87-89-8 0.100
p-Amino benzoic acid (PABA) 150-13-0 0.100
OTHERS
Adenine sulphate 321-30-2 20.000
Adenosine monophosphate 61-19-8 0.400
Adenosine triphosphate 56-65-5 2.000
Cholesterol 57-88-5 0.400
Deoxyribose 533-67-5 1.000
Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 0.100
Guanine hydrochloride 635-39-2 0.600
Hypoxanthine 68-94-0 0.708
Phenol Red 34487-61-1 30.000
Polysorbate 80 9005-65-6 9.800
Ribose 50-69-1 1.000
Sodium Pyruvate 113-24-6 0.220
Thymine 65-71-4 0.600
Uracil 66-22-8 0.600
Xanthine 69-89-6 0.688
Customization: pH, concentration, component additions, and nutrient modifications (glucose, salts, HEPES) available. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Manufactured under ISO 13485-certified and CE-approved facilities, with every lot released against compendial test methods.

verified

ISO 13485:2016 QMS

Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in an ISO Class 5 (Class 100) environment right after the final 0.04 µm polish.

assignment

Micro-Batch Precision

Small, tightly controlled batches for consistent composition and pH from lot to lot.

Endotoxin — USP <85> BET

Released at < 0.05 EU/mL.

Particulate — USP <788> Method 2

Subvisible particles ≥10 µm and ≥25 µm within compendial limits.

Osmolality — USP <785>

Measured per lot and reported on the CoA.

Documentation / CoA

Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.

A lot-specific Certificate of Analysis is available for every lot — request it at support@diagnocine.com.
Product Comparison

How DCP-M1992X compares

Same Medium 199 chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.

Parameter DCP-M1992X (FluxMPS™) Conventional Medium 199, Earle's (0.22 µm filtered) Standard Medium 199, Hanks' (0.22 µm filtered)
Formulation Complete Earle's Medium 199 (2 g/L glucose, L-glutamine, pyruvate, NaHCO₃); HEPES-free Earle's salts, bicarbonate-buffered Hanks' salts, for room-air use
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin specification < 0.05 EU/mL Often not specified at this level Often not specified at this level
USP <788> particulate compliance check_circle cancel cancel
Ultrapure Type 1 water (18.2 MΩ·cm) check_circle Varies Varies
ISO 13485:2016 manufacturing check_circle Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel cancel
FAQ

Frequently asked questions

Common questions about using DCP-M1992X in microfluidic and conventional culture.

Yes. DCP-M1992X is quadruple-stage filtered to a 0.04 µm final pore size, so it carries far fewer subvisible particles than 0.22 µm media — approximately 5× cleaner by particulate count. That lowers the risk of clogging microchannels, valves and membranes in organ-on-a-chip, tissue-chip and microfluidic perfusion systems.[3,6]
The medium is filtered four times: 0.1 µm twice and 0.04 µm twice. A single 0.22 µm pass can let mycoplasma and fine particulates through; the 0.04 µm stages act as a mycoplasma barrier and polish out sub-micron particles before ISO Class 5 aseptic fill.[8]
This is the classic Earle's formulation, buffered by 4.4 g/L sodium bicarbonate (2.2 g/L after 1:1 dilution to 1X) in a 5% CO₂ atmosphere, with no HEPES to interfere with pH-sensitive or HEPES-sensitive assays. HEPES can be added if you need extra buffering outside the incubator. Medium 199 supports short-term survival without serum, but long-term cultivation requires serum supplementation; review the literature for cell-line-specific requirements.[1]
Yes. Earle's salts with 4.4 g/L sodium bicarbonate (2.2 g/L after 1:1 dilution to 1X) are designed for a 5% CO₂ atmosphere. Keep time outside the incubator short, or choose a HEPES-containing FluxMPS™ Medium 199 variant.
Yes. Explanted tissue can survive in Medium 199 without serum, but long-term cultivation of cells requires serum supplementation; with serum the medium supports a wide variety of cells. Growth factors, antibiotics and other additives can be added as your protocol requires, and custom pH, concentration, component additions and nutrient modifications are available from support@diagnocine.com.
The endotoxin specification is < 0.05 EU/mL, tested by the USP <85> bacterial endotoxins test (BET) and reported on the lot Certificate of Analysis. The medium is made with Ultrapure Type 1 water (18.2 MΩ·cm).
Yes. Each lot ships with a CoA covering appearance (red), pH 7.4 (± 0.04), sterility (no bacterial or fungal growth after 14 days of incubation per USP), endotoxin and osmolality results. Request copies at support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed background on Medium 199, organ-on-a-chip culture, buffering and mycoplasma control.

  1. Morgan JF, Morton HJ, Parker RC. Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc Soc Exp Biol Med. 1950;73(1):1–8. doi:10.3181/00379727-73-17557
  2. Earle WR, Schilling EL, Stark TH, Straus NP, Brown MF, Shelton E. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J Natl Cancer Inst. 1943;4(2):165–212. doi:10.1093/jnci/4.2.165
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
  4. Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668. doi:10.1126/science.1188302
  5. Esch EW, Bahinski A, Huh D. Organs-on-chips at the frontiers of drug discovery. Nat Rev Drug Discov. 2015;14(4):248–260. doi:10.1038/nrd4539
  6. Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
  7. Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
  8. Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
  9. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. doi:10.1021/bi00866a011
  10. Zhang YS, Aleman J, Shin SR, et al. Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of drug toxicity. Proc Natl Acad Sci USA. 2017;114(12):E2293–E2302. doi:10.1073/pnas.1612906114
  11. Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029
  12. Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973;52(11):2745–2756. doi:10.1172/JCI107470

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