FluxMPS™ MCDB 153 Medium without Sodium Bicarbonate: 1X Liquid
FluxMPS™ MCDB 153 Medium without Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-M153H-B1X is an MPS-grade, ultra-filtered MCDB 153 −Bicarbonate +HEPES formulation engineered for serum-free or low-protein culture of Human Epidermal Keratinocytes and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media — ensuring trace elements are delivered particle-free and endotoxin is below TLR4 activation threshold. Formulation: [+] 1.081 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 28mM HEPES, [+] Trace Elements | [-] Sodium Bicarbonate.
- Trace element formulation — zinc, copper, selenium, manganese for metalloenzyme support and antioxidant activity in serum-free culture
- Optimized for: Human Epidermal Keratinocytes, CHO cells, Chicken Embryo Fibroblasts — CO₂-independent
- 28 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO₂ for open-top chips and atmospheric platforms
- 0.04 µm final nano-filtration — trace elements delivered as dissolved ions, not particulate aggregates
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Endotoxin < 0.05 EU/mL — below TLR4 activation threshold for sensitive primary cell models
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Cell typesHuman Epidermal Keratinocytes
- Glucose1081 mg/L (1.081 g/L)
- HEPES28 mM, pKa 7.3 at 37°C
- Trace ElementsPresent
- AppearanceRed colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)300.00–340.00 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Shelf Life12 months / 2–8°C
Engineered where standard media fails
MCDB media formulated for primary cells contain trace element salts that can form subvisible particulate aggregates when filtered at 0.22 µm under typical manufacturing conditions. These aggregates clog microfluidic channels, disrupt trace metal bioavailability, and activate innate immune signaling in sensitive primary cell models. FluxMPS™ eliminates these failure modes with four-stage sub-0.04 µm filtration and < 0.05 EU/mL endotoxin.
Particle-free trace element delivery
0.04 µm filtration ensures trace metal salts are fully dissolved — no aggregate deposition in chip channels or culture vessels where trace metal precipitation would alter bioavailability.
Serum-free primary cell support
Trace element composition supports metalloenzyme activity (SOD, GPx, carbonic anhydrase) critical for primary cell survival and function without serum supplementation.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) eliminates ionic contaminants that would compete with trace element uptake by low-serum primary cells.
Below TLR4 endotoxin threshold
< 0.05 EU/mL endotoxin — below the TLR4 activation threshold — prevents LPS-driven inflammatory signaling that confounds primary cell phenotype in serum-free conditions.
HEPES CO₂-stable pH
HEPES (28 mM, pKa 7.3 at 37°C) maintains stable pH for sensitive primary cell differentiation without CO₂ dependency.
Customization on demand
pH, glucose, trace element concentrations, HEPES, and growth factor co-formulations available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma purity and particle-free trace element delivery unavailable from conventional 0.22 µm filtered MCDB media.
-
1
0.1 µm Pre-filtration I — Large Particulate & Aggregate Removal
Removes large aggregates including trace metal salt precipitates; protects downstream 0.04 µm membranes and chip geometries from fouling.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) and residual fine particulates — absent from standard 0.22 µm filtration.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
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4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional MCDB media
FluxMPS™ DCP-M153H-B1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered MCDB 153 −Bicarbonate +HEPES, with confirmed mycoplasma barrier and particle-free trace element delivery at every production stage.
© Diagnocine® — DCP-M153H-B1X
Specialized cell models & OoC applications
FluxMPS™ DCP-M153H-B1X was formulated specifically for Human Epidermal Keratinocytes, CHO cells, Chicken Embryo Fibroblasts — CO₂-independent in low-protein or serum-free conditions. The 0.04 µm filtered trace element matrix enables safe use in microfluidic chip architectures where conventional MCDB media would cause trace metal particulate fouling.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered variant available for automated bioreactor perfusion where trace metal salt nanoparticulates in standard MCDB media cause valve fouling.
- Trace Metal Nanoparticulate Removal: 10 nm filtration removes sub-0.04 µm metal salt colloids invisible to standard QC
- Valve & Sensor Protection: Eliminates micro-fouling from trace element aggregates in automated systems
- Extended Perfusion Stability: Consistent trace element delivery over weeks-long primary cell culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
Organ-on-a-Chip & MPS
Ultra-clean 0.04 µm filtration prevents microchannel clogging. Trace elements delivered particle-free — no aggregate deposition in chip channels.
Skin-on-Chip
FluxMPS™ MCDB 153 −Bicarbonate +HEPES delivers sub-mycoplasma purity and trace element fidelity for demanding skin-on-chip models in serum-free or low-protein conditions.
Open-Top Epidermal OoC
FluxMPS™ MCDB 153 −Bicarbonate +HEPES delivers sub-mycoplasma purity and trace element fidelity for demanding open-top epidermal ooc models in serum-free or low-protein conditions.
CO₂-Independent CHO Culture
FluxMPS™ MCDB 153 −Bicarbonate +HEPES delivers sub-mycoplasma purity and trace element fidelity for demanding co₂-independent cho culture models in serum-free or low-protein conditions.
Keratinocyte Barrier Assays
FluxMPS™ MCDB 153 −Bicarbonate +HEPES delivers sub-mycoplasma purity and trace element fidelity for demanding keratinocyte barrier assays models in serum-free or low-protein conditions.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] 1.081 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 28mM HEPES, [+] Trace Elements | [-] Sodium Bicarbonate |
| Appearance | Red colored, clear solution |
| Glucose | 1081 mg/L (1.081 g/L) |
| HEPES | 28 mM (pKa 7.3 at 37°C) |
| Trace Elements | Present |
| pH USP <791> | 7.4 ± 0.04 |
| Osmolality USP <785> | 300.00–340.00 mOsm/kg H₂O |
| Total ingredients | 54 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent — 28 mM HEPES maintains pH without gas supplementation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
MCDB 153 −Bicarbonate +HEPES: 54 ingredients verified per lot with CAS numbers for full raw-material traceability. Contains trace elements for metalloenzyme support and antioxidant activity. HEPES (28 mM = 6672 mg/L approx.) listed under OTHERS.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Ammonium metavanadate | 7803-55-6 | 0.000585 |
| Calcium chloride dihydrate | 10035-04-8 | 4.411 |
| Cupric sulphate pentahydrate | 7758-99-8 | 0.00275 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 284.088 |
| Ferrous sulphate heptahydrate | 7782-63-0 | 1.390 |
| Magnesium chloride hexahydrate | 7791-18-6 | 122.000 |
| Manganese sulphate | 7785-87-7 | 0.000151 |
| Molybdic acid ammonium tetrahydrate | 12054-85-2 | 0.00124 |
| Nickel chloride | 7718-54-9 | 0.00012 |
| Potassium chloride | 7447-40-7 | 111.830 |
| Sodium acetate anhydrous | 127-09-3 | 301.530 |
| Sodium chloride | 7647-14-5 | 7599.000 |
| Stannous chloride monohydrate | 10025-69-1 | 0.000113 |
| Sodium metasillicate nonahydrate | 13517-24-3 | 0.1421 |
| Sodium selenite | 10102-18-8 | 0.0038 |
| Zinc sulphate heptahydrate | 7446-20-0 | 0.144 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.510 |
| L-Alanine | 56-41-7 | 8.910 |
| L-Arginine hydrochloride | 1119-34-2 | 210.700 |
| L-Asparagine monohydrate | 5794-13-8 | 15.000 |
| L-Aspartic acid | 56-84-8 | 3.990 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 42.040 |
| L-Glutamic acid | 56-86-0 | 14.710 |
| L-Glutamine | 56-85-9 | 877.200 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 16.770 |
| L-Isoleucine | 73-32-5 | 1.968 |
| L-Leucine | 61-90-5 | 65.600 |
| L-Lysine hydrochloride | 657-27-2 | 18.270 |
| L-Methionine | 63-68-3 | 4.480 |
| L-Phenylalanine | 63-91-2 | 4.960 |
| L-Proline | 147-85-3 | 34.530 |
| L-Serine | 56-45-1 | 63.060 |
| L-Threonine | 72-19-5 | 11.910 |
| L-Tryptophan | 73-22-3 | 3.060 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 3.410 |
| L-Valine | 72-18-4 | 35.130 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 13.960 |
| D-Biotin | 58-85-5 | 0.0146 |
| D-Pantothenic acid (hemicalcium) | 137-08-6 | 0.238 |
| Folic acid | 59-30-3 | 0.790 |
| Niacinamide | 98-92-0 | 0.03663 |
| Pyridoxine hydrochloride | 58-56-0 | 0.06171 |
| Riboflavin | 83-88-5 | 0.0376 |
| Thiamine hydrochloride | 67-03-8 | 0.337 |
| Vitamin B12 | 68-19-9 | 0.407 |
| myo-Inositol | 87-89-8 | 18.020 |
| OTHERS | ||
| Adenine hydrochloride | 2922-28-3 | 30.880 |
| D-Glucose | 50-99-7 | 1081.000 |
| HEPES | 7365-45-9 | 6600.000 |
| Phenol red sodium salt | 34487-61-1 | 1.242 |
| Putrescine dihydrochloride | 333-93-7 | 0.161 |
| Sodium pyruvate | 113-24-6 | 55.00 |
| Thioctic acid | 1077-28-7 | 0.206 |
| Thymidine | 50-89-5 | 0.727 |
Manufacturing & compliance
Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system, with particular attention to trace element dissolution and particulate removal during MCDB formulation.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — essential for trace element MCDB formulations where ionic contaminants compete with zinc, copper, and selenium uptake.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, per-lot traceability, Certificate of Analysis for every lot — trace element concentrations verified against specification.
Endotoxin — USP <85> BET
LAL assay < 0.05 EU/mL — below TLR4 activation threshold for primary cell cultures.
Particulate — USP <788> Method 2
Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Target: 300.00–340.00 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-M153H-B1X compares
FluxMPS™ DCP-M153H-B1X vs. conventional 0.22 µm–filtered MCDB 153 −Bicarbonate +HEPES formulations.
| Parameter | DCP-M153H-B1X (FluxMPS™) | Conventional MCDB 153 −Bicarbonate +HEPES (0.22 µm filtered) |
Standard Alt. MCDB (0.22 µm filtered) |
|---|---|---|---|
| MCDB 153 without Sodium Bicarbonate + HEPES — CO₂-independent keratinocyte and fibroblast OoC base | check_circle Yes | cancel No | cancel No |
| Trace element delivery | Particle-free (0.04 µm) | May contain aggregates | May contain aggregates |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Frequently asked questions
Common questions about FluxMPS™ DCP-M153H-B1X MCDB 153 −Bicarbonate +HEPES.
Supporting literature
Key publications supporting MCDB 153 −Bicarbonate +HEPES for Human Epidermal Keratinocytes culture and organ-on-a-chip applications.
- Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-6
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
