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FluxMPS™ MCDB 151 Medium: 1X Liquid

Product#: DCP-M151H1X
$71.49
DCP-M151H1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ MCDB 151 Medium: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate

FluxMPS™ DCP-M151H1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MCDB 151 + HEPES formulation engineered for serum-free or low-protein culture of Human Epidermal Keratinocytes, CHO cells and Chicken Embryo Fibroblasts on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final filter delivers trace elements as dissolved ions rather than particulate aggregates, and the endotoxin release specification is < 0.05 EU/mL. Formulation: [+] 1.081 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 28 mM HEPES, [+] Trace Elements.

  • Trace element formulation — zinc, copper, selenium and manganese for metalloenzyme support and antioxidant activity in serum-free culture
  • Formulated for Human Epidermal Keratinocytes, CHO cells and Chicken Embryo Fibroblasts
  • 28 mM HEPES (pKa 7.3 at 37°C) — pH stability without CO₂ dependence for open-top chips and atmospheric platforms
  • 0.04 µm final filtration — trace elements delivered as dissolved ions, not particulate aggregates
  • Quadruple-stage filtration train: 0.1 µm prefiltration I → 0.04 µm final filtration I → 0.1 µm prefiltration II → 0.04 µm final filtration II
  • Endotoxin release specification < 0.05 EU/mL by LAL assay (USP <85>)
  • Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ
CAT. NO.
DCP-M151H1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
MCDB 151 Medium: 1X Liquid
  • Cell typesHuman Epidermal Keratinocytes, CHO cells, Chicken Embryo Fibroblasts
  • Glucose1081 mg/L (1.081 g/L)
  • HEPES28 mM, pKa 7.3 at 37°C
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)320–360 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

MCDB media formulated with trace element salts can form subvisible particulate aggregates when filtered at 0.22 µm under typical manufacturing conditions. These aggregates can clog microfluidic channels, disrupt trace metal bioavailability, and contribute to innate immune signaling in sensitive primary cell models. FluxMPS™ is filtered through four sub-0.04 µm stages and is released to a < 0.05 EU/mL endotoxin specification.

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Particle-free trace element delivery

0.04 µm final filtration keeps trace metal salts fully dissolved — reducing the risk of aggregate deposition in chip channels or culture vessels where precipitation could alter bioavailability.

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Serum-free primary cell support

The trace element composition supports metalloenzyme activity (SOD, GPx, carbonic anhydrase) relevant to primary cell survival and function without serum supplementation.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) minimizes ionic and organic contaminants introduced during formulation of trace-element-containing media.

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Low endotoxin release specification

< 0.05 EU/mL by LAL assay (USP <85>) supports work with sensitive primary cell models such as keratinocytes and endothelial cells.

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HEPES-buffered pH stability

HEPES (28 mM, pKa 7.3 at 37°C) supports stable pH during pH-sensitive keratinocyte differentiation without requiring supplemental CO₂.

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Customization on demand

pH, glucose, trace element concentrations, HEPES, and growth factor co-formulations available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes reaching a final 0.04 µm polish, run as two prefilter-plus-final-filter pairs — each 0.04 µm final filter is protected by its own dedicated 0.1 µm prefilter. This delivers particle-free trace element delivery unavailable from conventional 0.22 µm filtered MCDB media.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and aggregates, including trace metal salt precipitates; protects the first 0.04 µm cartridge from fouling.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass through a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge and providing redundancy ahead of the final polish.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish under ISO Class 5 conditions.

Performance vs. conventional MCDB media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-sized organisms (0.2–0.3 µm) are retained by 0.04 µm barrier filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-M151H1X MCDB 151 Medium: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final Filtration I mycoplasma barrier, 0.1 μm Prefiltration II, 0.04 μm Final Filtration II Polish ? Microfluidics Suitable MCDB 151 + HEPES for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) ensuring particle-free trace element delivery.
© Diagnocine® — DCP-M151H1X
Applications

Specialized cell models & OoC applications

FluxMPS™ DCP-M151H1X was formulated for Human Epidermal Keratinocytes, CHO cells and Chicken Embryo Fibroblasts in low-protein or serum-free conditions. The 0.04 µm filtered trace element matrix supports use in microfluidic chip architectures where conventional MCDB media risk trace metal particulate fouling.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor perfusion applications where sub-0.04 µm trace metal salt colloids in standard MCDB media can contribute to valve fouling.

  • Trace Metal Nanoparticulate Reduction: 10 nm filtration targets sub-0.04 µm metal salt colloids not addressed by standard QC
  • Valve & Sensor Protection: Reduces micro-fouling risk from trace element aggregates in automated systems
  • Extended Perfusion Stability: Consistent trace element delivery over weeks-long primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

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Organ-on-a-Chip & MPS

Ultra-clean 0.04 µm filtration is designed to reduce microchannel clogging risk. Trace elements are delivered particle-free.

Human Epidermal KeratinocytesCHO cellsChicken Embryo Fibroblasts
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Skin-on-Chip

FluxMPS™ MCDB 151 + HEPES supports sub-mycoplasma-barrier purity and trace element fidelity for skin-on-chip models in serum-free or low-protein conditions.

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Keratinocyte OoC

Formulated for demanding keratinocyte organ-on-a-chip models requiring low-protein or serum-free conditions and stable HEPES-buffered pH.

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CHO Bioproduction

Trace element and amino acid profile supports CHO cell culture and glycosylation-dependent bioproduction workflows in microfluidic formats.

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Wound Healing Assays

Trace element and growth-factor-compatible formulation supports keratinocyte migration and wound-healing assay models in low-protein conditions.

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Live-Cell Imaging & Biosensors

Reduced particulate baseline from 0.04 µm filtration supports confocal imaging and biosensor/TEER integration on chip; not intended as an optical-clarity claim for this phenol red-containing formulation.

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate
Appearance Red-colored, clear solution
Glucose 1081 mg/L (1.081 g/L)
HEPES 28 mM (pKa 7.3 at 37°C)
Trace Elements Present (zinc, copper, selenium, manganese)
pH USP <791> 7.4
Osmolality USP <785> 320–360 mOsm/kg H₂O
Total ingredients 48 across 4 categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.04 µm barrier filtration (not tested per lot)
Particulate ≥10 µm USP <788> M1 NMT 25/mL
Particulate ≥25 µm USP <788> M1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement HEPES-buffered; reduced CO₂ dependence — stable pH without supplemental CO₂ for open-top/atmospheric platforms; optional low CO₂ (up to 5%) compatible with closed, humidified incubation
Pack sizes 500 mL, 1000 mL
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MCDB 151 + HEPES: 48 ingredients verified per lot with CAS numbers for raw-material traceability. Contains trace elements for metalloenzyme support and antioxidant activity. HEPES (28 mM ≈ 6600 mg/L) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 4.411
Cupric sulfate pentahydrate 7758-99-8 0.0025
Disodium hydrogen phosphate anhydrous 7558-79-4 284.080
Ferrous sulfate heptahydrate 7782-63-0 0.417
Magnesium chloride hexahydrate 7791-18-6 122.000
Potassium chloride 7447-40-7 111.830
Sodium acetate anhydrous 127-09-3 301.530
Sodium bicarbonate 144-55-8 1176.000
Sodium chloride 7647-14-5 7599.000
Zinc sulfate heptahydrate 7446-20-0 0.863
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.510
L-Alanine 56-41-7 8.910
L-Arginine hydrochloride 1119-34-2 210.700
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 3.990
L-Cysteine hydrochloride monohydrate 7048-04-6 42.040
L-Glutamic acid 56-86-0 14.710
L-Glutamine 56-85-9 877.200
L-Histidine hydrochloride monohydrate 5934-29-2 16.770
L-Isoleucine 73-32-5 1.968
L-Leucine 61-90-5 65.600
L-Lysine hydrochloride 657-27-2 18.270
L-Methionine 63-68-3 4.476
L-Phenylalanine 63-91-2 4.956
L-Proline 147-85-3 34.530
L-Serine 56-45-1 63.060
L-Threonine 72-19-5 11.910
L-Tryptophan 73-22-3 3.060
L-Tyrosine disodium salt dihydrate 69847-15-0 3.920
L-Valine 72-18-4 35.130
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 13.960
D-Biotin 58-85-5 0.015
D-Ca-Pantothenate 137-08-6 0.238
Folic acid 59-30-3 0.790
Niacinamide 98-92-0 0.037
Pyridoxine hydrochloride 58-56-0 0.061
Riboflavin 83-88-5 0.038
Thiamine hydrochloride 67-03-8 0.337
Vitamin B12 68-19-9 0.407
myo-Inositol 87-89-8 18.020
OTHERS
Adenine hydrochloride 2922-28-3 30.880
D-Glucose 50-99-7 1081.000
HEPES 7365-45-9 6600.000
Phenol red sodium salt 34487-61-1 1.242
Putrescine dihydrochloride 333-93-7 0.161
Sodium pyruvate 113-24-6 55.000
Thioctic acid 1077-28-7 0.206
Thymidine 50-89-5 0.727
Custom formulation: Growth factor co-formulations, adjusted trace element concentrations, custom glucose, and pH modifications available. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular attention to trace element dissolution and particulate removal during MCDB formulation.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — used in trace-element MCDB formulations where ionic contaminants could interfere with zinc, copper and selenium delivery.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

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Micro-Batch Precision

Small-batch production, per-lot traceability, and a Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL; assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Release specification: 320–360 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-M151H1X compares

FluxMPS™ DCP-M151H1X vs. conventional 0.22 µm-filtered MCDB 151 + HEPES formulations.

Parameter DCP-M151H1X (FluxMPS™) Conventional MCDB 151 + HEPES
(0.22 µm filtered)		 Standard Alt. MCDB
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
MCDB 151 with 28mM HEPES and Trace Elements — optimized for keratinocyte and CHO OoC models check_circle Yes cancel No cancel No
Trace element delivery Particle-free (0.04 µm) May contain aggregates May contain aggregates
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.04 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-M151H1X MCDB 151 + HEPES.

Yes. DCP-M151H1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels suited to microphysiological systems (MPS), organ-on-a-chip (OoC), tissue-on-a-chip (ToC) and lab-on-a-chip (LoC) platforms. Trace elements are delivered as dissolved ions rather than particulate aggregates, and the endotoxin release specification is < 0.05 EU/mL to limit inflammatory activation in sensitive primary cell models such as Human Epidermal Keratinocytes.
FluxMPS™ uses four sequential filtration passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II — reaching a 0.04 µm final cut-off instead of the 0.22 µm typical of conventional MCDB media. This retains sub-micron particulates, including trace metal salt microaggregates, that pass through a 0.22 µm filter and could otherwise deposit in microfluidic channels or alter trace element bioavailability.
MCDB 151 was developed for human epidermal keratinocytes, CHO cells and chicken embryo fibroblasts. The included trace elements (zinc, copper, selenium, manganese) support metalloenzyme activity relevant to keratinocyte cornification and CHO glycosylation pathways. 28 mM HEPES supports pH stability during keratinocyte differentiation. For additional supplementation — growth factors, dialyzed FBS protein, or adjusted trace element levels — contact support@diagnocine.com.
HEPES-buffered (28 mM); reduced CO₂ dependence — stable pH is maintained without supplemental CO₂ for open-top and atmospheric culture platforms. For closed, humidified incubation, an optional low CO₂ atmosphere (up to 5%) is compatible with this formulation.
Yes. This formulation supports low-protein or serum-free culture and can be supplemented with growth factors (EGF, bFGF, VEGF), dialyzed FBS protein (0.5–2%), or other defined additives. Serum and protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; defined, protein-free additions may use 0.1 µm. Do not use a 0.04 µm membrane for supplement filtration — it will retain serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85>, Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before release and must meet the release specification of < 0.05 EU/mL.
Yes. A full CoA per lot covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MCDB 151 + HEPES for keratinocyte culture and organ-on-a-chip applications.

  1. Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-6
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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