FluxMPS Iscove's Modified Dulbecco's Medium (IMDM), Low Glucose with 25mM HEPES w/o Phenol Red: 1X Liquid

Q: Is DCP-IMDMLGH-R1X suitable for organ-on-a-chip (OoC) applications?

Yes. No pH indicator is present, so pH should be monitored externally as needed. Review the literature for the requirements of your specific cell line.

Q: What endotoxin level should I expect, and how is it verified?

The release specification is less than 0.05 EU/mL, controlled per manufacturing batch and verified by LAL assay per USP 85 (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Each batch is tested before release, and the result for that batch is reported on the Certificate of Analysis.

verified ISO 13485 Certified Manufacturing

FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), Low Glucose with 25mM HEPES w/o Phenol Red: 1X Liquid

L-Glutamine Sodium Bicarbonate HEPES Calcium Magnesium Glucose Sodium Pyruvate Phenol Red

A Microfluidics Suitable, low-glucose Iscove's Modified Dulbecco's Medium (IMDM) re-engineered for microphysiological systems and microfluidic channels. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

Nano-filtered, microchannel-safe formulation engineered for microphysiological systems (MPS) and organ-on-a-chip (OoC) platforms
FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) for ultra-low particulate, sub-mycoplasma-scale polishing
Endotoxin release specification < 0.05 EU/mL by USP <85> BET, controlled per manufacturing batch
Iscove's Modified Dulbecco's Medium (low-glucose, 2 g/L); HEPES-buffered (25 mM) with 36 mM sodium bicarbonate, phenol red-free
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) for ultra-clean microfluidic perfusion
ISO Class 5 (Class 100) aseptic fill & finish under an ISO 13485:2016 quality management system
pH, glucose concentration, salts, HEPES, and nutrient composition available on request

DCP-IMDMLGH-R1X | Sizes: 500 mL, 1000 mL | Cell Culture Media

UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

Iscove's Modified Dulbecco's Medium (IMDM), Low Glucose with 25mM HEPES w/o Phenol Red: 1X Liquid, sterile-filtered

Glucose2000.000 mg/L
L-Glutamine584.000 mg/L
Sodium Pyruvate110.000 mg/L
pH (USP <791>)7.4
Osmolality (USP <785>)280 – 320 mOsm/kg H₂O
Endotoxin< 0.05 EU/mL
FiltrationQuadruple-stage (0.1 µm ×2 + 0.04 µm ×2)
Storage2–8 °C, protected from light
Shelf Life12 months from date of manufacture, unopened
ShippingCold-pack

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard 0.22 µm media fails

Conventional 0.22 µm-filtered media can carry subvisible particulates and mycoplasma-scale contaminants (0.2–0.3 µm) that accumulate in microfluidic channels, corrupt biosensor signals, and shorten perfusion runs. FluxMPS™ IMDM, Low Glucose + 25mM HEPES w/o Phenol Red is re-engineered for these microphysiological systems.^[1,2]

filter_alt

Microchannel-safe purity

A 0.04 µm final-polish filter targets subvisible particulates per USP <788> Method 1 (light obscuration), reducing the microchannel accumulation and clogging that degrade organ-on-a-chip experiments.

target

Total metabolic control

A defined carbon-source and nutrient backbone (glucose, L-glutamine, sodium pyruvate) supports Warburg-effect and metabolic-flux studies where standard serum-rich media confound the readout.

water_drop

Ultrapure-grade water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), reducing trace-metal and organic-carbon (TOC) background for consistent culture performance.

visibility

Low background for imaging

Ultra-low particulate baseline from the 0.04 µm final filter reduces optical scatter for confocal microscopy, biosensors, and TEER measurements. (Riboflavin is present in this formulation and contributes its own native fluorescence, which filtration does not remove.)

science

Rich, stable nutrient profile

IMDM's enriched amino-acid and vitamin profile, selenium, and sodium pyruvate are released on a micro-batch basis for lot-to-lot consistency.

tune

Customization on demand

pH, glucose, salts, HEPES, and nutrient composition can be adjusted on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

The FluxMPS™ purity architecture is built around a four-stage membrane train (0.1 µm ×2 + 0.04 µm ×2), positioning DCP-IMDMLGH-R1X as a ready-to-use, ultra-clean medium for microfluidic and organ-on-a-chip work at a purity level significantly exceeding conventional 0.22 µm media.^[2,3]

1

0.1 µmPrefiltration I

Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm cartridge.
2

0.04 µmFinal filtration I

First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
3

0.1 µmPrefiltration II

Second dedicated prefilter, protecting the second 0.04 µm cartridge.
4

0.04 µmFinal filtration II — Polish

Ultimate polishing filter; aseptic fill under an ISO Class 5 (Class 100) environment.

Performance vs. conventional media

5×

cleaner than 0.22 µm media (particulate count)

0.04

µm final-polish membrane

Sterility & mycoplasma assurance. No bacterial or fungal growth after 14 days of incubation per USP <71> specification. Mycoplasma-scale contaminants (0.2–0.3 µm) are addressed by the 0.1 µm and 0.04 µm mycoplasma-retentive filtration stages above; mycoplasma status is not tested per lot.

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS IMDM, Low Glucose with 25mM HEPES w/o Phenol Red: 1X Liquid (DCP-IMDMLGH-R1X) - Quadruple-stage filtration (0.1 micron x2 + 0.04 micron x2) ultra-filtered IMDM cell culture medium for organ-on-a-chip, microphysiological system (MPS) and microfluidic applications | Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration architecture — two 0.1 µm prefiltration passes followed by two 0.04 µm final-filtration passes for ultra-low-particulate, microchannel-safe media.
© Diagnocine® — DCP-IMDMLGH-R1X

Applications

Built for microphysiological & organ-on-a-chip research

IMDM, Low Glucose + 25mM HEPES w/o Phenol Red supports rapidly proliferating, high-density cultures — from erythroid progenitors, macrophages, and B/T lymphocytes to hybridomas — and is well suited to perfused MPS, metabolic, and imaging applications.^[1,4]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors and robotic perfusion platforms, an optional MPS Grade 0.01 µm (10 nm) six-stage ultra nano-filtered variant is available, adding 0.02 µm and 0.01 µm stages after this product's standard 0.04 µm polish, to push particulate exclusion further.

Total particulate exclusion for narrow microchannels and high-resolution optics
Valve & sensor protection across long unattended runs
Extended perfusion stability for multi-week organ-on-a-chip experiments

Inquiry Required: the optional MPS Grade 0.01 µm (10 nm) variant is made to order — contact support@diagnocine.com to request it.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low-particulate fluid for perfused chips and barrier models.

OoCToCBoCLoCMPS

Cancer Biology

Warburg Effect & Metabolic Research

Defined carbon-source backbone for tumor-metabolism studies.

MCF-7MDA-MB-231HeLaA549

Stem Cell Biology

iPSC-Derived Models

Consistent nutrient profile for differentiated iPSC cultures.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cells

Nutrient-rich medium for vessel-on-chip and primary cells.

HUVECsHAECsPrimary hepatocytes

Metabolomics

Metabolic Flux Analysis

Clean baseline for tracer and respirometry workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium; this formulation contains 36 mM sodium bicarbonate.

¹³C tracingNMR metabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate baseline for long-term imaging.

ConfocalBiosensorsTEER

Technical Specifications

Technical specifications

Representative specifications for DCP-IMDMLGH-R1X. Values are confirmed per batch on the Certificate of Analysis.

Physical & Chemical Parameters

Parameter	Specification
Formulation	Iscove's Modified Dulbecco's Medium (IMDM), 1X liquid (+25 mM HEPES, w/o phenol red); contains L-Glutamine, Sodium Bicarbonate, Calcium, Magnesium, Glucose, Sodium Pyruvate
Appearance	Pale-yellow colored, clear solution (phenol red-free)
pH USP <791>	7.4
Osmolality USP <785>	280 – 320 mOsm/kg H₂O
Glucose	2000.000 mg/L
L-Glutamine	584.000 mg/L
Sodium Pyruvate	110.000 mg/L
Phenol Red	Not included

Sterility, Purity & Safety Parameters

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL (batch release specification)
Sterility USP <71>	Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE. No bacterial or fungal growth observed after 14 days of incubation, per USP <71>.
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788>	Meets USP <788> Method 1 (light obscuration) limits
Particulate ≥25 µm USP <788>	Meets USP <788> Method 1 (light obscuration) limits
Water purity	Ultrapure Type 1 (18.2 MΩ·cm)
Manufacturing std. ISO	ISO 13485:2016; 21 CFR Part 820 (QMSR) aligned
Fill environment	ISO Class 5 (Class 100) aseptic

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8 °C, away from bright light
Freeze-thaw	Avoid repeated freeze-thaw
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold-pack
CO₂ requirement	Approximately 8% CO₂ (derived from 36 mM sodium bicarbonate at pH 7.4; 25 mM HEPES provides supplemental buffering)

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Cell-culture grade, traceable lots
Traceability	Full lot traceability with CoA
Manufacturing QMS	ISO 13485:2016 certified facility
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned; For Research Use Only (RUO)
Production method	Micro-batch, ISO Class 5 fill & finish
Intended use	For Research Use Only (RUO)

Formulation

Full composition (mg/L)

Complete defined formulation of DCP-IMDMLGH-R1X, released on a per-batch basis. All 41 components and their mg/L values are reproduced from the validated formulation.

INORGANIC SALTS
Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	219.000
Magnesium sulfate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	330.000
Potassium nitrate	7757-79-1	0.076
Sodium bicarbonate	144-55-8	3024.000
Sodium chloride	7647-14-5	4505.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	109.000
Sodium selenite	10102-18-8	0.0173

AMINO ACIDS
Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	30.000
L-Alanine	56-41-7	25.000
L-Arginine hydrochloride	1119-34-2	84.000
L-Asparagine	70-47-3	25.000
L-Aspartic acid	56-84-8	30.000
L-Cystine dihydrochloride	30925-07-6	91.240
L-Glutamic acid	56-86-0	75.000
L-Glutamine	56-85-9	584.000
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	104.800
L-Leucine	61-90-5	104.800
L-Lysine hydrochloride	657-27-2	146.200
L-Methionine	63-68-3	30.000
L-Phenylalanine	63-91-2	66.000
L-Proline	147-85-3	40.000
L-Serine	56-45-1	42.000
L-Threonine	72-19-5	95.200
L-Tryptophan	73-22-3	16.000
L-Tyrosine disodium salt	69847-45-6	104.200
L-Valine	72-18-4	93.600

VITAMINS / OTHERS
Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	4.000
D-Biotin	58-85-5	0.013
D-Ca-Pantothenate	137-08-6	4.000
Folic acid	59-30-3	4.000
Nicotinamide	98-92-0	4.000
Pyridoxal hydrochloride	65-22-5	4.000
Riboflavin	83-88-5	0.400
Thiamine hydrochloride	67-03-8	4.000
Vitamin B12	68-19-9	0.013
i-Inositol	87-89-8	7.200
OTHERS
D-Glucose	50-99-7	2000.000
HEPES	7365-45-9	5958.000
Sodium pyruvate	113-24-6	110.000

Need a custom formulation? pH, glucose, salts, HEPES, and nutrient composition can be adjusted — contact support@diagnocine.com.

Quality Assurance

Manufacturing & compliance

All final packaging, quality assurance, and testing for DCP-IMDMLGH-R1X are completed at the Diagnocine R&D and Quality Testing Center; customization and assembly are performed at Diagnocine Precision, Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Manufactured under an ISO 13485:2016 quality management system, 21 CFR Part 820 (QMSR) aligned.

water_drop

Ultrapure Type 1 Water

Formulated with 18.2 MΩ·cm Ultrapure Type 1 water for ultra-low ionic and organic background.

biotech

ISO Class 5 Fill & Finish

Final 0.04 µm polish and aseptic fill performed in an ISO Class 5 (Class 100) environment.

assignment

Micro-Batch Precision

Micro-batch production with per-batch release testing for lot-to-lot consistency and traceability.

Endotoxin — USP <85> BET

Bacterial endotoxin (LAL) testing to a < 0.05 EU/mL release specification, controlled per manufacturing batch.

Particulate — USP <788> Method 1

Subvisible particulate matter controlled to USP <788> Method 1 (light obscuration) limits.

Osmolality — USP <785>

Osmolality verified per batch within the specified range.

Documentation / CoA

A Certificate of Analysis accompanies every batch with measured QC values.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request.

A Certificate of Analysis (CoA) is available for every batch — request one at support@diagnocine.com.

Product Comparison

How DCP-IMDMLGH-R1X compares

DCP-IMDMLGH-R1X versus conventional 0.22 µm-filtered IMDM and a standard 0.22 µm-filtered DMEM-class alternative.

Parameter	DCP-IMDMLGH-R1X (FluxMPS™)	Conventional IMDM (0.22 µm)	Standard DMEM-class (0.22 µm)
Grade	Microfluidics Suitable (0.04 µm final)	Standard grade (0.22 µm)	Standard grade (0.22 µm)
Formulation enrichment	Iscove-enriched amino acids/vitamins + selenium, potassium nitrate	IMDM base	DMEM base
Final filtration pore size	0.04 µm (40 nm)	0.22 µm	0.22 µm
Number of filtration stages	4 (0.1 µm ×2 + 0.04 µm ×2)	1	1
Mycoplasma barrier filtration	check_circle	cancel	cancel
Endotoxin (release specification)	< 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance	check_circle	cancel	cancel
Water quality	Ultrapure Type 1 (18.2 MΩ·cm)	Type 1/2	Type 1/2
Manufacturing QMS	ISO 13485:2016	Varies	Varies
Microfluidic channel compatibility	check_circle	Limited	Limited
Custom formulation	check_circle	Limited	Limited

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about DCP-IMDMLGH-R1X for microphysiological and standard cell-culture workflows.

Is DCP-IMDMLGH-R1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-IMDMLGH-R1X is a Microfluidics Suitable medium, nano-filtered to a 0.04 µm final polish and formulated with Ultrapure Type 1 water specifically to minimize subvisible particulates that clog microchannels and corrupt biosensor signals, making it well suited to OoC, MPS, and microfluidic perfusion.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

Why is phenol red omitted, and how do I monitor pH without it?expand_more

In DCP-IMDMLGH-R1X, phenol red is omitted to give you control over your culture conditions and to remove a known source of optical/estrogenic interference. No pH indicator is present, so pH should be monitored externally (e.g., with a benchtop or inline pH meter) as needed. Review the literature for the requirements of your specific cell line.

Does this medium require a CO₂ incubator?expand_more

Yes. This formulation contains 36 mM sodium bicarbonate (3024 mg/L) together with 25 mM HEPES. To maintain pH 7.4, an approximately 8% CO₂ incubator atmosphere is required; the HEPES component provides additional buffering capacity during open-bench handling but does not eliminate the CO₂ requirement given the bicarbonate present.

Can DCP-IMDMLGH-R1X be supplemented with serum, growth factors, or other components?expand_more

Yes. IMDM contains no proteins, lipids, or growth factors, so it is typically supplemented with serum or serum-free additives. When adding serum or protein-containing supplements, pre-filter through a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm membrane for serum, as it will strip serum of the proteins and lipoproteins that make it functional.

What endotoxin level should I expect, and how is it verified?expand_more

The release specification is < 0.05 EU/mL, controlled per manufacturing batch and verified by LAL assay per USP <85> (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Each batch is tested before release, and the result for that batch is reported on the Certificate of Analysis.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. A CoA accompanies every batch of DCP-IMDMLGH-R1X with measured QC values such as appearance, pH, osmolality, sterility, and endotoxin. Request one at support@diagnocine.com.

Scientific References

Supporting literature

Peer-reviewed literature supporting the use of enriched, ultra-filtered media in microphysiological and metabolic research.

Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772.doi:10.1038/nbt.2989
Ronaldson-Bouchard K, Vunjak-Novakovic G. Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development. Cell Stem Cell. 2018;22(3):310–324.doi:10.1016/j.stem.2018.02.011
Halldorsson S, et al. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231.doi:10.1016/j.bios.2014.07.029
Iscove NN, Melchers F. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978;147(3):923–933.doi:10.1084/jem.147.3.923
Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324(5930):1029–1033.doi:10.1126/science.1160809
Nikolaev M, et al. Homeostatic mini-intestines through scaffold-guided organoid morphogenesis. Nature. 2020;585(7826):574–578.doi:10.1038/s41586-020-2724-8
Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90.doi:10.1023/A:1022913015916
Booth R, Kim H. Characterization of a microfluidic in vitro model of the blood-brain barrier. Lab Chip. 2012;12(10):1784–1792.doi:10.1039/c2lc40094d
Whitesides GM. The origins and the future of microfluidics. Nature. 2006;442(7101):368–373.doi:10.1038/nature05058
Low LA, Mummery C, Berridge BR, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361.doi:10.1038/s41573-020-0079-3

FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), Low Glucose with 25mM HEPES w/o Phenol Red: 1X Liquid

FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), Low Glucose with 25mM HEPES w/o Phenol Red: 1X Liquid

Engineered where standard 0.22 µm media fails

Microchannel-safe purity

Total metabolic control

Ultrapure-grade water

Low background for imaging

Rich, stable nutrient profile

Customization on demand

Quadruple-stage filtration system

0.1 µmPrefiltration I

0.04 µmFinal filtration I

0.1 µmPrefiltration II

0.04 µmFinal filtration II — Polish

Performance vs. conventional media

Built for microphysiological & organ-on-a-chip research

Automated Bioreactors & Robotics

Micro Physiological System (MPS) & Chip

Warburg Effect & Metabolic Research

iPSC-Derived Models

Endothelial & Primary Cells

Metabolic Flux Analysis

Microscopy & Optical Sensing

Technical specifications

Full composition (mg/L)

Manufacturing & compliance

ISO 13485:2016 QMS

Ultrapure Type 1 Water

ISO Class 5 Fill & Finish

Micro-Batch Precision

Endotoxin — USP <85> BET

Particulate — USP <788> Method 1

Osmolality — USP <785>

Documentation / CoA

How DCP-IMDMLGH-R1X compares

Frequently asked questions

Supporting literature

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