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FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid
FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid
A Microfluidics Suitable, high-glucose Iscove's Modified Dulbecco's Medium (IMDM) engineered for microphysiological systems (MPS), organ-on-a-chip (OoC) and microfluidic channels. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- High-glucose Iscove's Modified Dulbecco's Medium (IMDM) formulated with 25 mM HEPES and sodium bicarbonate (3024 mg/L); phenol red-free and L-glutamine-free
- Quadruple-stage filtration train — 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, four passes — for ultra-low particulate, microchannel-safe media
- Endotoxin release specification < 0.05 EU/mL by USP <85> Bacterial Endotoxins Test (LAL), controlled per manufacturing batch
- Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) and filled under an ISO Class 5 (Class 100) aseptic environment
- Enriched IMDM amino-acid and vitamin backbone plus sodium selenite and potassium nitrate, released on a per-lot basis with Certificate of Analysis
- Manufactured under an ISO 13485:2016 quality management system; final packaging and QC at Diagnocine Precision, Totowa, NJ
- pH, glucose concentration, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com
- Glucose4500.000 mg/L
- L-GlutamineNot included
- Sodium Pyruvate110.000 mg/L
- pH (USP <791>)7.4
- Osmolality (USP <785>)250 - 290 mOsm/kg H₂O
- Endotoxin< 0.05 EU/mL
- FiltrationQuadruple-stage (0.1 μm ×2 + 0.04 μm ×2)
- Storage2-8°C, away from bright light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold-pack
Engineered where standard 0.22 μm media fails
Conventional 0.22 μm-filtered media can carry subvisible particulates and mycoplasma-scale contaminants (the smallest mycoplasma are approximately 0.2–0.3 μm) that accumulate in microfluidic channels, corrupt biosensor signals, and shorten perfusion runs. FluxMPS™ IMDM, High Glucose + 25mM HEPES w/o L-Glutamine, Phenol Red is re-engineered for these microphysiological systems.[1,2]
Microchannel-safe purity
A 0.04 μm final-polish filter targets subvisible particulates per USP <788> Method 1 (light obscuration), preventing the microchannel accumulation and clogging that degrade organ-on-a-chip experiments.
Total metabolic control
A defined carbon-source and nutrient backbone — 4500 mg/L glucose and 110 mg/L sodium pyruvate — supports Warburg-effect and metabolic-flux studies where standard serum-rich media confound the readout.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), controlling trace-metal and organic carbon (TOC) background in the finished medium.
Low background for imaging
Ultra-low-particulate fluid reduces optical scatter from particulates for confocal microscopy and biosensor work. Riboflavin and other formulation components contribute native fluorescence, so autofluorescence should be characterized empirically for your assay.
Rich, stable nutrient profile
IMDM's enriched amino-acid and vitamin profile, sodium selenite, and potassium nitrate are released on a per-lot basis for lot-to-lot consistency.
Customization on demand
pH, glucose, salts, HEPES, and nutrient composition can be adjusted on request — contact support@diagnocine.com.
Quadruple-stage filtration system
The FluxMPS™ purity architecture for DCP-IMDMH-QR1X is built around a validated four-pass membrane train — two dedicated 0.1 μm prefiltration passes, each protecting its own 0.04 μm final filter — positioning this medium as an ultra-clean, Microfluidics Suitable option for microfluidic and organ-on-a-chip work at a purity level conventional 0.22 μm media do not reach.[2,3]
-
1
0.1 μmPrefiltration I
Large particulate, cell debris and protein aggregate removal; protects the first 0.04 μm cartridge.
-
2
0.04 μmFinal filtration I
First 0.04 μm pass; retains sub-micron particulates and microaggregates that pass a 0.22 μm filter.
-
3
0.1 μmPrefiltration II
Second dedicated prefilter, protecting the second 0.04 μm cartridge.
-
4
0.04 μmFinal filtration II — Polish
Ultimate polishing filter; aseptic fill and finish under ISO Class 5 (Class 100) conditions.
Performance vs. conventional media
© Diagnocine® — DCP-IMDMH-QR1X
Built for microphysiological & organ-on-a-chip research
IMDM, High Glucose + 25mM HEPES w/o L-Glutamine, Phenol Red supports rapidly proliferating, high-density cultures — from erythroid progenitors, macrophages, and B/T lymphocytes to hybridomas — and is well suited to perfused MPS, metabolic, and imaging applications.[1,4]
Automated Bioreactors & Robotics
For automated bioreactors and robotic perfusion platforms, an optional 10 nm (0.01 μm) ultra nano-filtered MPS Grade variant is available to push particulate exclusion beyond the standard 0.04 μm polish supplied here.
- Total particulate exclusion for narrow microchannels and high-resolution optics
- Valve & sensor protection across long unattended runs
- Extended perfusion stability for multi-week organ-on-a-chip experiments
Inquiry Required: the optional 0.01 μm (10 nm) MPS Grade variant is made to order — contact support@diagnocine.com to request it.
Micro Physiological System (MPS) & Chip
Ultra-low-particulate fluid for perfused chips and barrier models.
Warburg Effect & Metabolic Research
Defined carbon-source backbone for tumor-metabolism studies.
iPSC-Derived Models
Consistent nutrient profile for differentiated iPSC cultures.
Endothelial & Primary Cells
Low-background medium for vessel-on-chip and primary cells.
Metabolic Flux Analysis
Clean baseline for tracer and respirometry workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Low-particulate baseline for long-term imaging workflows.
Technical specifications
Representative specifications for DCP-IMDMH-QR1X. Values are confirmed per lot on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | IMDM, 1X liquid; contains sodium bicarbonate (3024 mg/L), 25 mM HEPES, calcium, magnesium, high glucose (4500 mg/L) and sodium pyruvate (110 mg/L); without L-glutamine, without phenol red |
| Appearance | Pale-yellow colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 250 - 290 mOsm/kg H₂O |
| Glucose | 4500.000 mg/L |
| L-Glutamine | Not included |
| Sodium Pyruvate | 110.000 mg/L |
| Phenol Red | Not included |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification — see §Manufacturing & Compliance) |
| Sterility USP <71> | Filtered using 0.1 μm membrane TWICE and 0.04 μm membrane TWICE in a sterile environment. No bacterial or fungal growth observed after 14 days of incubation, per USP specification. |
| Mycoplasma | 0.1 μm mycoplasma-retentive filtration (not tested per lot); smallest known mycoplasma ≈ 0.2–0.3 μm |
| Particulate ≥10 μm USP <788> | Meets USP <788> Method 1 (light obscuration) limits |
| Particulate ≥25 μm USP <788> | Meets USP <788> Method 1 (light obscuration) limits |
| Water purity | Ultrapure Type 1 (18.2 MΩ·cm) |
| Manufacturing std. ISO | ISO 13485:2016; 21 CFR Part 820 (QMSR) aligned |
| Fill environment | ISO Class 5 (Class 100) aseptic |
| Parameter | Specification |
|---|---|
| Storage temperature | 2-8 °C, away from bright light |
| Freeze-thaw | Avoid repeated freeze-thaw |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold-pack |
| CO₂ requirement | Approximately 8% CO₂ required (sodium bicarbonate-buffered, ≈36 mM NaHCO₃); 25 mM HEPES provides supplemental buffering for bench-top handling outside the incubator |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell-culture grade, traceable lots |
| Traceability | Full lot traceability with CoA |
| Manufacturing QMS | ISO 13485:2016 certified facility |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, ISO Class 5 fill & finish |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
All 40 components across 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others) are reproduced below, released on a per-lot basis, organized into 3 browsable tabs.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 219.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 330.000 |
| Potassium nitrate | 7757-79-1 | 0.076 |
| Sodium bicarbonate | 144-55-8 | 3024.000 |
| Sodium chloride | 7647-14-5 | 4505.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Sodium selenite | 10102-18-8 | 0.0173 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Alanine | 56-41-7 | 25.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Asparagine | 70-47-3 | 25.000 |
| L-Aspartic acid | 56-84-8 | 30.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 91.240 |
| L-Glutamic acid | 56-86-0 | 75.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 104.800 |
| L-Leucine | 61-90-5 | 104.800 |
| L-Lysine hydrochloride | 657-27-2 | 146.200 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Proline | 147-85-3 | 40.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.200 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 104.200 |
| L-Valine | 72-18-4 | 93.600 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Biotin | 58-85-5 | 0.013 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| Vitamin B12 | 68-19-9 | 0.013 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4500.000 |
| HEPES buffer | 7365-45-9 | 5958.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
All final packaging, quality assurance, and testing for DCP-IMDMH-QR1X are completed at the Diagnocine R&D and Quality Testing Center; customization and assembly are performed at Diagnocine Precision, Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Manufactured under an ISO 13485:2016 quality management system; 21 CFR Part 820 (QMSR) aligned.
Ultrapure Type 1 Water
Formulated with 18.2 MΩ·cm Ultrapure Type 1 water for ultra-low ionic and organic background.
ISO Class 5 Fill & Finish
Final 0.04 μm polish and aseptic fill performed in an ISO Class 5 (Class 100) environment.
Micro-Batch Precision
Micro-batch production with per-lot release testing for lot-to-lot consistency and traceability.
Endotoxin USP <85> BET
Bacterial endotoxin testing (LAL) to a < 0.05 EU/mL release specification, controlled per manufacturing batch.
Particulate USP <788> Method 1
Subvisible particulate matter controlled to USP <788> Method 1 (light obscuration) limits.
Osmolality USP <785>
Osmolality verified per lot within the specified range.
Documentation / CoA
A Certificate of Analysis accompanies every lot with measured QC values.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-IMDMH-QR1X compares
DCP-IMDMH-QR1X versus conventional 0.22 μm-filtered IMDM and a standard 0.22 μm-filtered DMEM-class alternative.
| Parameter | DCP-IMDMH-QR1X (FluxMPS™) | Conventional IMDM (0.22 μm) | Standard DMEM-class (0.22 μm) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 μm final cut-off) | Not applicable | Not applicable |
| Formulation enrichment | Iscove-enriched amino acids/vitamins + selenium, potassium nitrate | IMDM base | DMEM base |
| Final filtration pore size | 0.04 μm (40 nm) | 0.22 μm | 0.22 μm |
| Number of filtration stages | 4 (0.1 μm ×2 + 0.04 μm ×2) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Type 1/2 | Type 1/2 |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | Limited | Limited |
| Custom formulation | check_circle | Limited | Limited |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about DCP-IMDMH-QR1X for microphysiological and standard cell-culture workflows.
Supporting literature
Peer-reviewed literature supporting the use of enriched, ultra-filtered media in microphysiological and metabolic research.
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772.doi:10.1038/nbt.2989
- Ronaldson-Bouchard K, Vunjak-Novakovic G. Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development. Cell Stem Cell. 2018;22(3):310-324.doi:10.1016/j.stem.2018.02.011
- Halldorsson S, et al. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218-231.doi:10.1016/j.bios.2014.07.029
- Iscove NN, Melchers F. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978;147(3):923-933.doi:10.1084/jem.147.3.923
- Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324(5930):1029-1033.doi:10.1126/science.1160809
- Nikolaev M, et al. Homeostatic mini-intestines through scaffold-guided organoid morphogenesis. Nature. 2020;585(7826):574-578.doi:10.1038/s41586-020-2724-8
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75-90.doi:10.1023/A:1022913015916
- Booth R, Kim H. Characterization of a microfluidic in vitro model of the blood-brain barrier. Lab Chip. 2012;12(10):1784-1792.doi:10.1039/c2lc40094d
- Whitesides GM. The origins and the future of microfluidics. Nature. 2006;442(7101):368-373.doi:10.1038/nature05058
- Low LA, Mummery C, Berridge BR, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345-361.doi:10.1038/s41573-020-0079-3