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FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture

Product#: DCP-CM-B1X
$71.49
DCP-CM-B1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without HEPES

FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. Formulated as an Eagle-Hanks' Amino Acids (EHAA)-based Click's Medium without sodium bicarbonate or beta-mercaptoethanol, it is processed through Diagnocine's validated four-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm), delivering microchannel-safe purity with substantially lower particulate load than single-pass 0.22 µm filtration.

  • Quadruple-stage filtration: 0.1 µm (Prefiltration I) → 0.04 µm (Final filtration I) → 0.1 µm (Prefiltration II) → 0.04 µm (Final filtration II — Polish)
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET)
  • EHAA-based Click's Medium formulation; bicarbonate-free and beta-mercaptoethanol-free; pH 7.4 (USP <791>)
  • Prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
  • ISO Class 5 aseptic fill & finish; manufactured under an ISO 13485:2016 quality management system
  • Mycoplasma risk mitigated via 0.1 µm and 0.04 µm serial filtration (not tested per lot)
  • Contains 1000 mg/L glucose, 584 mg/L L-glutamine, 275 mg/L sodium pyruvate, and phenol red pH indicator (11 mg/L)
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-CM-B1X | Cell Culture Media Sizes: 500 mL, 1000 mL UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture
  • Glucose1000 mg/L (1.0 g/L)
  • L-Glutamine584 mg/L
  • Sodium Pyruvate275 mg/L
  • HEPESNot added
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)320 - 360 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ is engineered to reduce these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filter stage retains sub-mycoplasma-sized particulates; USP <788> Method 1 (light obscuration) particulate compliance verified per batch.

target

Total metabolic control

Defined glucose (1.0 g/L), L-glutamine, and sodium pyruvate concentrations support precise nutrient control for metabolic flux and Warburg-effect studies.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm), controlled for trace-metal and total organic carbon (TOC) content to minimize non-biological background.

visibility

Low background for imaging

Quadruple-stage filtration minimizes particulate background, supporting reliable confocal microscopy and biosensor readouts. Note: this formulation contains phenol red, which may interfere with fluorescence- or absorbance-based assays; a phenol-red-free custom formulation is available on request.

science

Rich, stable nutrient profile

Micro-batch precision manufacturing locks in amino acid and vitamin concentrations, ensuring lot-to-lot reproducibility critical for long-term perfusion studies.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture is processed through a validated four-stage filtration train that reaches a 0.04 µm final pore size — addressing mycoplasma-sized particulates, subvisible debris, and bioburden that single-pass 0.22 µm filtration cannot address.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final filter cartridge downstream.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates — including material in the mycoplasma size range (0.2–0.3 µm) — that pass through a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter stage, protecting the second 0.04 µm final filter cartridge and providing redundancy against upstream breakthrough.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filtration prior to aseptic fill & finish in an ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

By reaching a 0.04 µm final pore size across two dedicated prefilter + final-filter pairs, FluxMPS™ delivers substantially lower particulate counts than single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every production batch.

4
Sequential prefilter + final-filter passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is mitigated via 0.1 µm and 0.04 µm serial filtration (not tested per lot); a mycoplasma assay result is reported only when performed per lot and requested.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture (DCP-CM-B1X) Quadruple-stage filtration system diagram showing four sequential stages: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, and 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic cell culture applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering sub-mycoplasma purity for MPS and OoC applications.
© Diagnocine® — DCP-CM-B1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture is validated for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: The 0.01 µm grade is available by special order. Contact support@diagnocine.com to request this variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation prevents microchannel clogging and maintains laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined carbon source and low-endotoxin background enables precise metabolic flux analysis and Warburg effect studies.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity essential for maintaining endothelial barrier integrity and TEER values in perfusion models.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined carbon source and nutrient background support isotope tracing and NMR-based metabolomics studies. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Quadruple-stage filtration reduces particulate background for high-content confocal imaging and optical biosensor integration; this formulation contains phenol red, which may affect fluorescence/absorbance-based readouts.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture undergoes the complete quality-release battery listed below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Contains L-Glutamine (584 mg/L), Phenol Red (11 mg/L), Calcium (245.53 mg/L as CaCl2·2H2O), Magnesium (164.9 mg/L MgCl2 + 97.72 mg/L MgSO4), Glucose (1000 mg/L), Sodium Pyruvate (275 mg/L); without Sodium Bicarbonate, HEPES, beta-Mercaptoethanol
Appearance Clear, red to pink-red solution (phenol red pH indicator present)
pH USP <791> 7.4
Osmolality USP <785> 320 - 360 mOsm/kg H2O
Glucose 1000 mg/L (1.0 g/L)
L-Glutamine 584 mg/L
Sodium Pyruvate 275 mg/L
Phenol Red Present (11 mg/L, phenol red sodium salt)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification — see §Manufacturing & Compliance)
Sterility USP <71> No growth after 14 days
Mycoplasma Risk mitigated by 0.1 µm and 0.04 µm filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016 ISO 13485
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Bicarbonate-free formulation; CO2 incubator not required for pH stability (phosphate-buffered, Hank's-based); standard CO2 conditions remain compatible if preferred
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Available pack sizes 500 mL, 1000 mL
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers. All ingredient names and mg/L values are reproduced from the manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 245.530
Disodium hydrogen phosphate 7558-79-4 47.900
Magnesium chloride anhydrous 7786-30-3 164.900
Potassium chloride 7447-40-7 400.000
Potassium dihydrogen phosphate 7778-77-0 60.000
Sodium chloride 7647-14-5 8000.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Alanine 56-41-7 35.600
L-Arginine hydrochloride 1119-34-2 317.500
L-Asparagine anhydrous 70-47-3 52.800
L-Aspartic acid 56-84-8 53.200
L-Cystine dihydrochloride 30189-89-0 78.200
L-Glutamine 56-85-9 584.000
L-Glutamic acid 56-86-0 58.800
L-Histidine hydrochloride monohydrate 5934-29-2 104.700
L-Isoleucine 73-32-5 130.000
L-Leucine 61-90-5 130.000
L-Lysine hydrochloride 657-27-2 181.200
L-Methionine 63-68-3 37.500
L-Phenylalanine 63-91-2 80.000
L-Proline 147-85-3 46.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 120.000
L-Tryptophan 73-22-3 25.000
L-Tyrosine disodium salt dihydrate 69847-45-6 118.900
L-Valine 72-18-4 115.000
Component CAS Number mg/L
VITAMINS
Calcium pantothenate 137-08-6 2.000
Choline chloride 67-48-1 2.000
Folic acid 59-30-3 2.000
Niacinamide 98-92-0 2.000
Pyridoxal hydrochloride 65-22-5 2.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
Adenosine 58-61-7 25.000
Cytosine 71-30-7 25.000
Glucose 50-99-7 1000.000
Guanosine 118-00-3 25.000
Magnesium sulfate anhydrous 7487-88-9 97.720
myo-Inositol 87-89-8 4.000
Phenol red sodium salt 34487-61-1 11.000
Sodium pyruvate 113-24-6 275.000
Uridine 58-96-8 25.000
Customization: pH, glucose concentration, salt balance, HEPES concentration, and full nutrient profile are available on request. Contact support@diagnocine.com with your specifications.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering all manufacturing, testing, and release processes for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity Type 1 water, controlled for trace-metal and total organic carbon (TOC) content.

biotech

ISO Class 5 Fill & Finish

Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing locks in lot-to-lot nutrient consistency critical for reproducible perfusion studies and long-term OoC experiments.

Endotoxin — USP <85> BET

Controlled per manufacturing batch via LAL assay; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance per batch.

Osmolality — USP <785>

Freezing-point osmometry performed per USP <785>. Result: 320 - 360 mOsm/kg H2O.

Documentation — CoA & Full Lot Records

Certificate of Analysis available for every batch, including full QC panel, raw material traceability, and release signatures.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request your lot-specific CoA at support@diagnocine.com with your lot number and order reference.
Product Comparison

How DCP-CM-B1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter DCP-CM-B1X (FluxMPS™) Conventional Click (0.22 µm) Standard Click alternative
Grade Microfluidics Suitable Not applicable Not applicable
Base Formulation Click's Medium (EHAA) w/o Sodium Bicarbonate, Mercaptoethanol Click Standard Click Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages (0.1 µm ×2 + 0.04 µm ×2) 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture.

Yes. DCP-CM-B1X is processed through our Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), yielding low particulate counts that help prevent microchannel clogging in OoC and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm diameter) or substantial subvisible particulates. FluxMPS™ uses four sequential stages reaching 0.04 µm, addressing these contaminants and delivering substantially lower particulate counts per USP <788> Method 1.
This is a Click's Medium (EHAA) formulation based on Eagle-Hanks' Amino Acids, buffered by the phosphate salts in the base rather than sodium bicarbonate. Beta-mercaptoethanol is excluded from the base because it is typically added fresh at the time of use due to its instability in solution. Contact support@diagnocine.com for a custom formulation with bicarbonate, HEPES, or mercaptoethanol added per your protocol.
This formulation is bicarbonate-free and does not contain HEPES; it relies on the phosphate salts of the Hank's-based formulation for pH buffering. A CO2 incubator is not required for pH stability, though standard CO2 conditions remain compatible if your protocol calls for them.
Yes. FluxMPS™ Click's Medium (EHAA) can be supplemented with FBS, growth factors, antibiotics, or other additives per standard cell culture practice. Add supplements immediately before use. When filtering serum or protein-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF filter — never a 0.04 µm membrane, which retains serum proteins and lipoproteins and will clog rapidly.
Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay per USP <85> BET (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before shipment. Batch-specific results are documented in the Certificate of Analysis available on request from support@diagnocine.com.
Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting the scientific rationale for ultra-filtered cell culture media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
  6. Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
  7. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  8. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  9. Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
  10. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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