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FluxMPS™ CMRL 1066 Medium: 2X Liquid

Product#: DCP-CMRL2X
$162.25
DCP-CMRL2X
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FluxMPS™ CMRL 1066 Medium: 2X Liquid

Double-strength (2X) CMRL 1066 — a chemically defined, nucleoside- and vitamin-rich medium for pancreatic islet, primary-cell and organ culture — ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a sodium bicarbonate buffer, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.

  • 2X concentrate: dilute to 1X or mix with an equal volume of a compatible solution, cell suspension, agar or matrix
  • Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
  • 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
  • Endotoxin < 0.05 EU/mL (USP <85> BET)
  • Formulation: [+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red
  • Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
  • Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
DCP-CMRL2X|UNSPSC 41116155Cell culture media
Catalog No. DCP-CMRL2X · Size: 500 mL
CMRL 1066 Medium: 2X Liquid
  • D-Glucose2,000 mg/L
  • L-Glutamine200 mg/L
  • BufferNaHCO₃ 5,500 mg/L
  • Phenol Red42.48 mg/L
  • AppearanceRed
  • pH7.4 (± 0.04)
  • Endotoxin< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8 °C, protect from light
  • Format2X liquid, 500 mL
ISO 13485:2016USP <85> <785> <788>RUO
Why FluxMPS™

Engineered where standard media fails

Conventional CMRL 1066 is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and add noise to optical readouts.[9,10] FluxMPS™ CMRL 1066 keeps the original formulation and removes that failure mode.

filter_alt

Microchannel-safe purity

A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.

target

Total metabolic control

Defined 2,000 mg/L D-glucose and 200 mg/L L-glutamine in the 2X (half that at 1X), with no sodium pyruvate.

water_drop

Ultrapure-grade water

Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.

visibility

Low background for imaging

Particle-depleted medium reduces scatter and debris artifacts in confocal imaging and on-chip sensing.

science

Nucleoside- and cofactor-rich

58 components, including deoxynucleosides, NAD, NADP, FAD, coenzyme A, cocarboxylase, glutathione, cholesterol and Tween 80.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Every lot of DCP-CMRL2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X CMRL 1066 polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.

  1. 01

    0.1 µmPre-filtration I

    Removes large particulates and aggregates and extends the life of the downstream filters.

  2. 02

    0.04 µmPre-filtration II

    Retains fine particulates, bacteria and mycoplasma.

  3. 03

    0.1 µmSterile-filtration I

    Second-pass redundancy that catches anything shed or bypassed upstream.

  4. 04

    0.04 µmSterile-filtration II — Final Polish

    Final nano-polish immediately before ISO Class 5 aseptic fill.

Performance vs. conventional media

By particulate count, FluxMPS™ CMRL 1066 is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.

5×
cleaner by particulate count vs. 0.22 µm media
0.04
µm final filtration pore size
Sterility & mycoplasma assurance: no bacterial or fungal growth is observed after 14 days of incubation, as per USP specification (USP <71>), and the 0.04 µm stages provide USP <63>-equivalent mycoplasma assurance.[9]
FluxMPS™ CMRL 1066 Medium: 2X Liquid (DCP-CMRL2X) Quadruple-stage filtration system diagram: 0.1 μm pre-filtration, 0.04 μm pre-filtration, 0.1 μm sterile filtration and 0.04 μm final polish for MPS-grade CMRL 1066 cell culture media used in pancreatic islet, primary-cell, organ-on-a-chip and microfluidic applications, Diagnocine
Figure 1. Quadruple-stage filtration: 0.1 µm pre-filtration, 0.04 µm pre-filtration, 0.1 µm sterile filtration and 0.04 µm final polish before ISO Class 5 fill.
© Diagnocine® — DCP-CMRL2X
Applications

Where FluxMPS™ CMRL 1066 fits

CMRL 1066 is a chemically defined, nucleoside- and vitamin-rich cell culture medium originally developed for the long-term cultivation of mouse L cells without protein supplementation.[1] It can also be supplemented with serum to support fibroblast-like cells and other mammalian cell types and is used in pancreatic islet, primary-cell, organ-culture, and general cell-biology research.[2]

CMRL 1066 Medium 2X is a double-strength formulation designed to be diluted to 1X or mixed with an equal volume of another compatible solution while maintaining the intended final nutrient concentration. The 2X concentration is useful when incorporating high-volume supplements, treatment solutions, cell suspensions, agar, or other matrices that would otherwise dilute a standard 1X medium.

This format is suitable for customized culture-media preparation, semisolid cultures, tissue and organ culture, pancreatic-islet studies, fibroblast-like cell culture, and other specialized mammalian-cell research. It should generally be adjusted to a final 1X concentration before use unless a validated protocol specifies otherwise.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.

  • Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
  • Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
  • Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.

Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.

Islet Biology

Pancreatic Islet Culture

A widely used base for maintaining isolated pancreatic islets in research workflows, with serum or defined supplements.[2]

Human isletsRodent isletsβ-cells
Primary Culture

Primary Cells & Organ Culture

Supports primary mammalian cells, tissue explants and organ culture where a nutrient-rich defined base is needed.[1]

Primary cellsExplantsOrgan culture
Cell Biology

Fibroblasts & Mouse L Cells

Originally developed for long-term, protein-free cultivation of mouse L cells; with serum it supports fibroblast-like cells.[1,3]

L cellsFibroblasts
Semisolid Culture

Agar & Matrix Cultures

The 2X format mixes 1:1 with agar, hydrogels or other matrices so the final culture sits at 1X nutrient strength.

AgarHydrogels3D matrices
Microfluidics

Islet-on-Chip & MPS

Microchannel-safe medium for perfused islet-on-chip and other organ-on-a-chip platforms.[4,5,6,7,8]

OoCIslet-on-chipMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Fewer particles means cleaner fields for confocal imaging, calcium imaging and on-chip biosensors.

ConfocalCa²? imagingBiosensors
Technical Specifications

Specifications & quality control

Release specifications for DCP-CMRL2X. Lot-specific values are reported on the Certificate of Analysis.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red
Concentration 2X (dilute to 1X)
Appearance Red
pH 7.4 (± 0.04) USP <791>
Osmolality Measured per lot; reported on CoA USP <785>
D-Glucose 2,000 mg/L (2.0 g/L)
L-Glutamine 200 mg/L
Sodium Bicarbonate 5,500 mg/L
Phenol Red 42.48 mg/L
Sodium Pyruvate Not included
HEPES Not included
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin < 0.05 EU/mL USP <85> BET
Sterility No bacterial or fungal growth after 14 days of incubation USP <71>
Mycoplasma 0.04 µm barrier filtration USP <63> equiv.
Particulate ≥10 µm Meets limits USP <788> M2
Particulate ≥25 µm Meets limits USP <788> M2
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100) ISO
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8 °C, protected from light
Freeze-thaw Do not freeze
Shelf life 12 months; use before the expiry date on the product label
Shipping condition Ice Pack
CO₂ requirement CO₂-controlled incubator (typically 5% CO₂) after dilution to 1X; sodium bicarbonate 5,500 mg/L in the 2X, 2,750 mg/L at 1X
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade High-purity, cell-culture-tested components
Traceability Lot-traceable; CoA per lot
Manufacturing QMS ISO 13485:2016 ISO
Regulatory alignment 21 CFR Part 820 (cGMP) aligned
Production method Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ
Intended use For Research Use Only (RUO)
Note: pH and sodium bicarbonate concentration of the prepared medium are critical factors affecting cell growth. This is also influenced by amount of medium and volume of culture vessel used (surface to volume ratio). For example, in large bottles, such as Roux bottles, pH tends to rise perceptibly as a significant volume of carbon dioxide is released. Therefore, optimal conditions of pH, sodium bicarbonate concentration and surface to volume ratio must be determined for each cell type. We recommend stringent monitoring of pH. If needed, pH can be adjusted by using sterile 1N HCl or 1N NaOH or by bubbling in carbon dioxide. If required, supplements can be added to the medium prior to or after filter sterilization observing sterility precautions.
Formulation

Full composition (mg/L)

DCP-CMRL2X is 2X CMRL 1066 Medium with 2.0 g/L glucose, L-glutamine, sodium bicarbonate and Phenol Red. It does not contain sodium pyruvate nor HEPES. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 58 components are listed below at 2X strength; values are per-lot release basis and are halved after dilution to 1X.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 530.000
Magnesium sulphate anhydrous 7487-88-9 195.380
Potassium chloride 7447-40-7 800.000
Sodium acetate anhydrous 127-09-3 100.000
Sodium bicarbonate 144-55-8 5500.000
Sodium chloride 7647-14-5 13600.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 244.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 100.000
L-Alanine 56-41-7 50.000
L-Arginine 74-79-3 115.740
L-Aspartic acid 56-84-8 60.000
L-Cysteine hydrochloride monohydrate 7048-04-6 520.000
L-Cystine 56-89-3 40.000
L-Glutamic acid 56-86-0 150.000
L-Glutamine 56-85-9 200.000
L-Histidine hydrochloride monohydrate 5934-29-2 40.000
L-Isoleucine 73-32-5 40.000
L-Leucine 61-90-5 120.000
L-Lysine hydrochloride 657-27-2 140.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 50.000
L-Proline 147-85-3 80.000
L-Serine 56-45-1 50.000
L-Threonine 72-19-5 60.000
L-Tryptophan 73-22-3 20.000
L-Tyrosine 60-18-4 80.000
L-Valine 72-18-4 50.000
Trans-4-Hydroxy-L-Proline 51-35-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Biotin 58-85-5 0.020
D-Ca-Pantothenate 137-08-6 0.020
Folic acid 59-30-3 0.020
L-Ascorbic acid sodium salt 134-03-2 100.000
myo-Inositol 87-89-8 0.100
Niacinamide 98-92-0 0.050
Nicotinic acid 59-67-6 0.050
p-Amino benzoic acid (PABA) 150-13-0 0.100
Pyridoxal hydrochloride 65-22-5 0.050
Pyridoxine hydrochloride 58-56-0 0.050
Riboflavin 83-88-5 0.020
Thiamine hydrochloride 67-03-8 0.020
OTHERS
2' Deoxyadenosine 958-09-8 20.000
2' Deoxycytidine hydrochloride 3992-42-5 23.200
2' Deoxyguanosine 961-07-9 20.000
5-Methyldeoxycytidine 838-07-3 0.200
Beta NAD 53-84-9 14.000
Beta NADP sodium salt 1184-16-3 20.000
Cholesterol 57-88-5 0.400
Cocarboxylase 154-87-0 2.000
Coenzyme A sodium salt 55672-92-9 5.000
D-Glucose 50-99-7 2000.000
D-Glucuronic acid sodium salt 14984-34-0 7.760
FAD disodium salt 84366-81-4 0.212
Glutathione 70-18-8 20.000
Phenol Red 34487-61-1 42.480
Thymidine 50-89-5 20.000
Tween 80 9005-65-6 10.000
Uridine-5-Triphosphate sodium salt 19817-92-6 2.000
Customization: Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.

verified

ISO 13485:2016 QMS

Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.

assignment

Micro-Batch Precision

Small, tightly controlled batches for consistent composition and pH from lot to lot.

Endotoxin — USP <85> BET

Released at < 0.05 EU/mL.

Particulate — USP <788> Method 2

Subvisible particles ≥10 µm and ≥25 µm within compendial limits.

Osmolality — USP <785>

Measured per lot and reported on the CoA.

Documentation / CoA

Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.

A lot-specific Certificate of Analysis is available for every lot — request it at support@diagnocine.com.
Product Comparison

How DCP-CMRL2X compares

Same CMRL 1066 chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.

Parameter DCP-CMRL2X (FluxMPS™) Conventional CMRL 1066, 1X (0.22 µm filtered) Standard RPMI 1640 (0.22 µm filtered)
Formulation 2X CMRL 1066 with L-glutamine, NaHCO₃ and Phenol Red; complete formulation 1X CMRL 1066, ready to use General-purpose medium, also used for islets
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin specification < 0.05 EU/mL Often not specified at this level Often not specified at this level
USP <788> particulate compliance check_circle cancel cancel
Ultrapure Type 1 water (18.2 MΩ·cm) check_circle Varies Varies
ISO 13485:2016 manufacturing check_circle Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel cancel
FAQ

Frequently asked questions

Common questions about using DCP-CMRL2X for islet, primary-cell, microfluidic and conventional culture.

Yes. DCP-CMRL2X is quadruple-stage filtered to a 0.04 µm final pore size, so it carries far fewer subvisible particles than 0.22 µm media — approximately 5× cleaner by particulate count. That lowers the risk of clogging microchannels, valves and membranes in islet-on-chip and other organ-on-a-chip and microfluidic perfusion systems.[4,5,6,7]
The medium is filtered four times: 0.1 µm twice and 0.04 µm twice. A single 0.22 µm pass can let mycoplasma and fine particulates through; the 0.04 µm stages act as a mycoplasma barrier and polish out sub-micron particles before ISO Class 5 aseptic fill.[9]
This is the complete CMRL 1066 formulation — L-glutamine, sodium bicarbonate and Phenol Red included — with no sodium pyruvate or HEPES, so you add them only if your protocol calls for them. Most cell types also need serum or defined growth supplements. Users are advised to review the literature for supplementation and physiological growth requirements specific to each cell type.[1]
Yes, once diluted to 1X. The medium is bicarbonate-buffered (2,750 mg/L at 1X), so use a CO₂-controlled incubator (typically 5% CO₂) and keep time in room air short.
Dilute the 2X to 1X, or mix it with an equal volume of another compatible solution — a high-volume supplement, treatment solution, cell suspension, agar or other matrix — so the final nutrient concentration stays at 1X. It should generally be adjusted to a final 1X concentration before use unless a validated protocol specifies otherwise. Serum and other supplements can be added before or after filter sterilization, observing sterility precautions.
The endotoxin specification is < 0.05 EU/mL, tested by the USP <85> bacterial endotoxins test (BET) and reported on the lot Certificate of Analysis. Low endotoxin matters for islets and primary cells, which can respond to trace LPS. The medium is made with Ultrapure Type 1 water (18.2 MΩ·cm).
Yes. Each lot ships with a CoA covering appearance (red), pH 7.4 (± 0.04), sterility (no bacterial or fungal growth after 14 days of incubation per USP), endotoxin and osmolality results. Request copies at support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed background on CMRL 1066, pancreatic islet culture, organ-on-a-chip culture and mycoplasma control.

  1. Parker RC, Castor LN, McCulloch EA. Altered cell strains in continuous culture: a general survey. In: Cellular Biology, Nucleic Acids and Viruses. Spec Publ N Y Acad Sci. 1957;5:303–313.
  2. Ricordi C, Lacy PE, Finke EH, Olack BJ, Scharp DW. Automated method for isolation of human pancreatic islets. Diabetes. 1988;37(4):413–420. doi:10.2337/diab.37.4.413
  3. Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
  4. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
  5. Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
  6. Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
  7. Ingber DE. Human organs-on-chips for disease modelling, drug development and personalized medicine. Nat Rev Genet. 2022;23(8):467–491. doi:10.1038/s41576-022-00466-9
  8. Esch EW, Bahinski A, Huh D. Organs-on-chips at the frontiers of drug discovery. Nat Rev Drug Discov. 2015;14(4):248–260. doi:10.1038/nrd4539
  9. Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
  10. Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029
  11. Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668. doi:10.1126/science.1188302

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