Earle's Balanced Salt Solution w/ Sodium bicarbonate w/o Phenol red: 1X
Cat No.: DCP-EBSSB-R1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] Sodium Bicarbonate
[-] Phenol Red
Description:
Earle's balanced salt solution (EBSS) is a fundamental isotonic saline solution formulated by W.R. Earle in 1943. This solution provides an essential environment that maintains cellular structural and physiological integrity in laboratory settings. The carefully balanced composition of inorganic salts, combined with glucose as an energy source, makes EBSS a critical component in various cell culture applications. This premium Earle's Balanced Salt Solution (EBSS) provides a high-clarity, colorless physiological medium designed for superior optical performance in cell culture applications. Formulated as a ready-to-use 1X liquid solution with sodium bicarbonate buffer but without phenol red indicator, this specialized EBSS delivers reliable cell maintenance with enhanced compatibility for sensitive optical and fluorescence-based assays.
Advantages:
- Superior Optical Performance: Colorless formulation eliminates interference in imaging and detection systems
- Experimental Flexibility: Compatible with fluorescent probes across multiple wavelengths
- Ready-to-Use Convenience: 1X formulation eliminates preparation time and reduces risk of formulation errors
- CO₂-Dependent Buffering: Sodium bicarbonate provides optimal pH maintenance in standard cell culture environments
- Enhanced Data Quality: Reduces background and improves signal-to-noise ratio in optical assays
Applications:
- Fluorescence Microscopy: Eliminates potential autofluorescence interference from phenol red
- Cell Imaging Applications: Provides maximum optical clarity for high-resolution cell imaging
- Colorimetric Assays: Prevents spectral overlap with phenol red that could affect assay readings
- Flow Cytometry: Ensures clearer analysis without background fluorescence
Composition:
| Ingredients |
mg/L |
| Calcium Chloride Dihydrate |
265.000 |
| Magnesium Sulfate Anhydrous |
97.720 |
| Sodium Phosphate Monobasic |
122.000 |
| Potassium Chloride |
400.000 |
| Sodium Bicarbonate |
2200.000 |
| Sodium Chloride |
6800.000 |
| D-Glucose |
1000.000 |
Quality Control:
- Appearance: Red colored, clear solution.
- pH: 7.6
- Osmolality: Approximately 265-305 mOsm/kg H2O
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 2-8 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
