Earle's Balanced Salt Solution w/ Sodium bicarbonate w/o Phenol red: 1X
FluxMPS™ Earle's Balanced Salt Solution w/ Sodium Bicarbonate w/o Phenol Red: 1X
An MPS-grade, ready-to-use 1X Earle's Balanced Salt Solution (EBSS) buffered with sodium bicarbonate and formulated without phenol red for maximum optical clarity. Quadruple-stage 0.1-micron and 0.04-micron membrane filtration delivers a microchannel-safe, ultra-clean isotonic buffer for organ-on-a-chip, microfluidic, and sensitive imaging workflows.
- Filtered 0.1-micron membrane twice and 0.04-micron membrane twice for quadruple-stage, microchannel-safe purity
- Endotoxin specification: NMT 1 EU/mL
- Precise pH 7.6, sodium bicarbonate buffered for standard CO2 incubator environments
- Osmolality maintained at approximately 265-305 mOsm/kg H2O
- Phenol red-free, colorless formulation engineered for fluorescence, imaging, and colorimetric assays
- Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm) under ISO 13485-certified, CE-approved facilities
- Microchannel-safe for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and other MPS platforms
- Custom pH, concentration, and additive formulations available on request
- Size
- 500 mL
- pH
- 7.6
- Osmolality
- Approx. 265-305 mOsm/kg H2O
- Buffer System
- Sodium Bicarbonate
- Endotoxin
- NMT 1 EU/mL
- Sterility
- No growth after 14 days (USP)
- Toxicity Test
- Passes
- Filtration
- 0.1 µm x2 + 0.04 µm x2
- Storage
- 2-8°C, protect from light
- Shelf Life
- 24 months
Engineered where standard buffers fail
Conventional 0.22 µm-filtered balanced salt solutions can carry subvisible particulates, drift in pH or osmolality, and introduce optical background from indicators like phenol red. FluxMPS™ DCP-EBSSB-R1X addresses each failure mode with quadruple-stage filtration, a defined sodium bicarbonate buffering system, and a colorless, phenol red-free formulation.
Microchannel-Safe Purity
Sequential 0.1 µm and 0.04 µm membrane filtration removes finer particulates than a single 0.22 µm pass, protecting narrow microfluidic channels from accumulation.
Precise, Stable pH
Formulated to pH 7.6 with a sodium bicarbonate buffering system tuned for standard CO2-controlled culture environments.
Ultrapure-Grade Water
Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm) aligned with USP <85> expectations for sensitive cell and molecular biology work.
Low Background for Imaging & Assays
The phenol red-free, colorless formulation eliminates spectral interference in fluorescence microscopy, flow cytometry, and colorimetric assays.
Defined, Traceable Composition
Each lot is prepared to the same balanced salt formulation, including calcium chloride dihydrate, magnesium sulfate, sodium phosphate monobasic, potassium chloride, sodium bicarbonate, sodium chloride, and D-glucose.
Customization on Demand
Alternate pH, concentrations, or the addition of chemicals, compounds, proteins, or supplements can be prepared on request.
Quadruple-stage filtration system
Every DiagnoCine Precision sterile buffer, including DCP-EBSSB-R1X, is filter-sterilized through a 0.1-micron membrane twice and a 0.04-micron membrane twice — a quadruple-stage architecture that helps guard against mycoplasma-scale contamination, the smallest type of which can be about 0.2 microns.
- 1
0.1 µm Pre-filtration I
Large particulate and aggregate removal, extending the life of downstream filters.
- 2
0.04 µm Pre-filtration II
Fine particulate and bioburden retention, sized well below the roughly 0.2-micron diameter of the smallest mycoplasma.
- 3
0.1 µm Sterile-filtration I
Second-pass redundancy through a 0.1-micron membrane.
- 4
0.04 µm Sterile-filtration II – Final Polish
Ultimate polish pass through a 0.04-micron membrane ahead of ISO Class 5 aseptic fill.
Performance vs. conventional buffer
A single 0.22-micron pass, typical of conventional balanced salt solutions, is the industry baseline. Sequential 0.1-micron (x2) and 0.04-micron (x2) filtration removes finer particulates than that single 0.22-micron pass, supporting the low-particulate demands of microfluidic and organ-on-a-chip channels.
© Diagnocine® – DCP-EBSSB-R1X
Where DCP-EBSSB-R1X performs
A phenol red-free, sodium bicarbonate buffered EBSS formulated for cell and molecular biology workflows where optical clarity, isotonic balance, and low particulate burden matter.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant of this EBSS formulation can be prepared for automated bioreactors and robotic liquid-handling platforms where valve and sensor protection is critical.
- Total Particulate Exclusion: minimizes fine debris that can foul microvalves and sensors
- Valve & Sensor Protection: reduces wear on precision automated fluidics hardware
- Extended Perfusion Stability: supports longer unattended perfusion runs
Inquiry Required: the 0.01 µm ultra-filtered grade is prepared to order – contact support@diagnocine.com to discuss your automated platform requirements.
Micro Physiological System (MPS) & Chip
Isotonic, low-particulate buffer for microfluidic chip perfusion and rinsing.
Wash, Dilution & Reconstitution
Balanced salt solution suited to rinse, dilution, and reconstitution steps in cell-based workflows.
iPSC-Derived Model Handling
Colorless, phenol red-free buffer for handling of sensitive iPSC-derived cell models.
Endothelial & Primary Cell Perfusion
Isotonic wash and perfusion buffer for endothelial and primary cell culture systems.
ELISA, Blotting & Blocking
Colorless formulation avoids spectral overlap in colorimetric plate-based assays.
Microscopy & Optical Sensing
Phenol red-free formulation eliminates autofluorescence interference during live-cell imaging.
Full specification sheet
All values below are as measured and released for DCP-EBSSB-R1X, this Earle's Balanced Salt Solution.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Earle's Balanced Salt Solution with sodium bicarbonate buffer, without phenol red indicator |
| Appearance | Red colored, clear solution |
| pH USP <791> | 7.6 |
| Osmolality USP <785> | Approximately 265-305 mOsm/kg H2O |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | NMT 1 EU/mL |
| Sterility USP <71> | No bacterial or fungal growth observed after 14 days of incubation |
| Toxicity Test | Passes |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facilities |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2-8°C, away from bright light |
| Shelf Life | 24 months |
| Expiry | Use before expiry date given on the product label |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485:2016 | ISO 13485-certified facilities (Suppliers of DiagnoCine Precision) |
| Regulatory Alignment | CE-approved facilities |
| Production Method | Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center; customization and assembly performed at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | For Research Use Only (RUO); not intended for clinical, diagnostic, or therapeutic use in humans |
Full composition (mg/L)
Every component is released on a per-lot basis to the concentrations listed below.
| Component | CAS Number | Concentration (mg/L) |
|---|---|---|
| Calcium Chloride Dihydrate (CaCl2·2H2O) | 10035-04-8 | 265.000 |
| Magnesium Sulfate Anhydrous (MgSO4) | 7487-88-9 | 97.720 |
| Sodium Phosphate Monobasic (NaH2PO4) | 7558-80-7 | 122.000 |
| Potassium Chloride (KCl) | 7447-40-7 | 400.000 |
| Sodium Bicarbonate (NaHCO3) | 144-55-8 | 2200.000 |
| Sodium Chloride (NaCl) | 7647-14-5 | 6800.000 |
| D-Glucose | 50-99-7 | 1000.000 |
Manufacturing & compliance
DCP-EBSSB-R1X is manufactured, filled, and tested under a documented quality system spanning raw materials through final release.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).
ISO Class 5 Fill & Finish
Aseptic fill following quadruple-stage 0.1 µm and 0.04 µm membrane filtration.
Micro-Batch Precision
Final packaging, QA, and testing performed at the DiagnoCine R&D and Quality Testing Center; customization completed at DiagnoCine Precision, Totowa, New Jersey, USA.
Endotoxin USP <85> BET
NMT 1 EU/mL.
Sterility USP <71>
No bacterial or fungal growth observed after 14 days of incubation.
Osmolality USP <785>
Approximately 265-305 mOsm/kg H2O.
Documentation / CoA
A Certificate of Analysis is available for this lot.
How DCP-EBSSB-R1X compares
A comparison against a conventional single-pass, 0.22 µm-filtered balanced salt solution.
| Parameter | DCP-EBSSB-R1X (FluxMPS™) | Conventional version (0.22 µm filtered buffer) | Standard alternative (0.22 µm filtered buffer) |
|---|---|---|---|
| Sodium bicarbonate CO2-buffered formulation | check_circle | cancel | cancel |
| Final filtration pore size | 0.04 µm (final stage) | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Endotoxin specification | NMT 1 EU/mL certified | cancel | cancel |
| Phenol red-free, colorless formulation | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | cancel | cancel |
| Manufacturing QMS | ISO 13485:2016 | cancel | cancel |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-EBSSB-R1X, this Earle's Balanced Salt Solution.
Supporting literature
Curated peer-reviewed literature relevant to balanced salt solutions, buffer chemistry, and microphysiological system applications.
- Earle WR. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J Natl Cancer Inst. 1943.doi:10.1093/jnci/4.2.165
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772.doi:10.1038/nbt.2989
- Zhang B, Radisic M. Organ-on-a-chip devices advance to market. Lab Chip. 2017;17(14):2395-2420.doi:10.1039/C6LC01554A
- Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. 7th ed. Wiley-Blackwell; 2016.doi:10.1002/9781118873686
- Michl J, Park KC, Swietach P. Evidence-based guidelines for controlling pH in mammalian live-cell culture systems. Commun Biol. 2019;2:144.doi:10.1038/s42003-019-0393-7
- Baker M. Reproducibility crisis: blame it on the antibodies. Nature. 2015;521:274-276.doi:10.1038/521274a
- Ryan JA. Understanding and managing cell culture contamination. Corning Technical Bulletin. 1994.doi:10.13140/RG.2.1.3654.0964
- Uphoff CC, Drexler HG. Detection of mycoplasma contamination in cell cultures. Methods Mol Biol. 2011;731:93-103.doi:10.1007/978-1-61779-080-5_8
- Berthier E, Young EW, Beebe D. Engineers are from PDMS-land, biologists are from polystyrenia. Lab Chip. 2012;12(7):1224-1237.doi:10.1039/C2LC20982A