Earle's Balanced Salt Solution w/ Phenol red and Sodium bicarbonate: 1X
Cat No.: DCP-EBSSRB1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] Sodium Bicarbonate, [+] Phenol Red
Description:
Earle's balanced salt solution (EBSS) is a fundamental isotonic saline solution formulated by W.R. Earle in 1943. This solution provides an essential environment that maintains cellular structural and physiological integrity in laboratory settings. The carefully balanced composition of inorganic salts, combined with glucose as an energy source, makes EBSS a critical component in various cell culture applications. This ready-to-use Earle's Balanced Salt Solution (EBSS) is formulated to provide optimal support for mammalian cell culture applications. Supplied as a 1X concentration liquid, this solution offers convenience and reliability for your research needs.
Advantages:
- Convenience: Ready-to-use format saves time and reduces preparation errors
- Versatility: Supports various cell culture applications
- Visual pH Monitoring: Phenol red indicator allows for easy assessment of solution conditions
- CO₂-Dependent Buffering: Sodium bicarbonate provides optimal pH maintenance in standard cell culture environments
Applications:
- Short-term cell maintenance in CO₂ environments
- Washing cells before dissociation or counting
- Transporting cells or tissues
- General purpose physiological buffer for cell culture procedures
Composition:
| Ingredients |
mg/L |
| Calcium Chloride Dihydrate |
265.000 |
| Magnesium Sulfate Anhydrous |
97.720 |
| Sodium Phosphate Monobasic |
122.000 |
| Potassium Chloride |
400.000 |
| Sodium Bicarbonate |
2200.000 |
| Sodium Chloride |
6800.000 |
| D-Glucose |
1000.000 |
| Phenol Red Sodium Salt |
11.000 |
Quality Control:
- Appearance: Red colored, clear solution.
- pH: 7.6
- Osmolality: Approximately 265-305 mOsm/kg H2O
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 2-8 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
