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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-DMEMLH-QPB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM Low Glucose formulation with 25 mM HEPES, prepared without L-Glutamine, Sodium Pyruvate, or Sodium Bicarbonate. Four sequential filtration passes reach a 0.04 µm final pore size — well below the 0.22 µm threshold of conventional media — delivering approximately 5× lower particulate counts by count and supporting microphysiological systems (MPS), organ-on-a-chip (OoC), and other microfluidic tissue models. HEPES (25 mM, pKa 7.3 at 37°C) provides CO₂-independent pH stability.
- Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final polish for microfluidic-channel compatibility
- Low Glucose formulation (1000 mg/L D-Glucose) with 25 mM HEPES buffering (pKa 7.3 at 37°C) for CO₂-independent pH control
- Formulated without L-Glutamine, Sodium Pyruvate, and Sodium Bicarbonate for full metabolic and buffer customization at point of use
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> Bacterial Endotoxins Test), controlled per manufacturing batch
- 31 verified ingredients across inorganic salts, amino acids, vitamins, and other components, released against a full Certificate of Analysis
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Microfluidics Suitable grade (0.04 µm final cut-off) — engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and lab-on-a-chip (LoC) platforms
- Custom pH, glucose, salts, and nutrient adjustments available on request
- Formulation[+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Low Glucose | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Sodium Pyruvate
- AppearanceOrange-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Mycoplasma0.1 µm mycoplasma-retentive filtration
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Sterile, 4 stages)
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and endotoxin-associated debris that can accumulate in microfluidic channels and interfere with sensor signals. FluxMPS™ addresses these failure modes with a four-stage filtration train reaching a 0.04 µm final pore size. HEPES buffering removes CO₂ dependency for open-top chip architectures and atmospheric incubation.
Microchannel-safe purity
0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance supports reliable perfusion across a range of chip geometries.
Total metabolic control
L-Glutamine, Sodium Pyruvate, and Sodium Bicarbonate are excluded so glucose, buffer, and nitrogen sources can be defined precisely by the end user.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon (TOC) content, minimizing extraneous contaminant load in every batch.
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy and optical biosensor platforms. This formulation contains phenol red; phenol-red-free custom formulations are available on request for autofluorescence-sensitive assays.
Rich, stable nutrient profile
31 ingredients verified per lot across inorganic salts, amino acids, vitamins, and other components; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, HEPES, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration passes reach a final 0.04 µm polish, achieving purity levels not reached by conventional single-pass 0.22 µm filtration. The train runs as two dedicated prefilter + final-filter pairs in series, giving full redundancy.
-
1
0.1 µm Prefiltration I
Removes large aggregates and particulates; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I — Mycoplasma-retentive
First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Performance vs. conventional media
A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
© Diagnocine® — DCP-DMEMLH-QPB1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMLH-QPB1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. The 25 mM HEPES buffer makes it well suited to open-top microfluidic devices and atmospheric CO₂ environments.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) six-stage ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor perfusion and robotic liquid handlers — a distinct, higher tier from the 0.04 µm Microfluidics Suitable grade described on this page.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
0.04 µm–filtered media reduces microchannel clogging risk in complex multi-organ chip architectures.
CHO & Mammalian Cell Culture
Suited to CHO, cancer cell lines, primary cells, and clonal growth studies requiring a low-glucose, HEPES-buffered base.
iPSC-Derived Models
Low endotoxin release specification (< 0.05 EU/mL) and mycoplasma-retentive filtration support sensitive iPSC protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled media for HUVEC monolayer integrity studies.
Metabolic Flux Analysis
Defined nutrient background supports ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate count supports confocal microscopy and optical biosensor platforms. This formulation contains phenol red; phenol-red-free custom formulations are available on request.
Analytical release specifications
Every lot is released against the full specification matrix below. CoA available on request: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Low Glucose | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Sodium Pyruvate |
| Appearance | Orange-colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H₂O |
| D-Glucose | 1000 mg/L (Low Glucose) |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| L-Glutamine | Not added |
| Sodium Pyruvate | Not added |
| Sodium Bicarbonate | Not added |
| Phenol Red | 15.9 mg/L (present) |
| Total ingredients | 31 (3 tabs, 4 category groups) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release — see §Manufacturing) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent; HEPES-buffered (25 mM, pKa 7.3 at 37°C) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
31 ingredients verified per lot with CAS numbers for raw-material traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm Type 1 water with controlled trace-metal and TOC content, minimizing extraneous contaminant load in every batch.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 310–350 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMLH-QPB1X compares
FluxMPS™ DCP-DMEMLH-QPB1X vs. conventional 0.22 µm–filtered DMEM Low Glucose + HEPES formulations.
| Parameter | DCP-DMEMLH-QPB1X (FluxMPS™) | Conventional DMEM Low Glucose + HEPES (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm final cut-off) | Not specified | Not specified |
| HEPES-only — no L-Glutamine, no Pyruvate, no Bicarbonate | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm, 2 stages) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL | Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Yes | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMLH-QPB1X.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j