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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEMH-PR1X is a Microfluidics Suitable, ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and CO₂-variable or open-top microfluidic platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides supplemental pH buffering alongside the retained sodium bicarbonate system. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] Sodium Pyruvate, [-] Phenol Red.
- High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and other aerobically active cells
- 25 mM HEPES (pKa 7.3 at 37°C) — supplemental pH buffering alongside the bicarbonate system for handling outside the incubator
- 0.04 µm final filtration — retains particulates and organisms in the mycoplasma size range (0.2–0.3 µm) for microfluidic channels below 100 µm
- Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
- Endotoxin batch release specification < 0.05 EU/mL (LAL, USP <85>)
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 aseptic fill
- Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
- Formulation[+] High Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25mM HEPES, [-] Sodium Pyruvate, [-] Phenol Red
- AppearanceColorless to pale yellow, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL (batch release spec)
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes fine particulates and organisms in the mycoplasma size range that clog microfluidic channels. Standard DMEM also relies solely on bicarbonate buffering, causing pH drift whenever chips are handled outside a CO₂ incubator. FluxMPS™ addresses both failure modes.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion in sub-100 µm channel geometries.
High-energy cell support
4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.
HEPES: supplemental pH stability
25 mM HEPES (pKa 7.3 at 37°C) helps maintain pH during handling outside a CO₂ incubator — useful for open-top chips, point-of-care devices, and bench-top sampling.
Low background for imaging
Quadruple-stage 0.04 µm filtration keeps particulate background low for confocal microscopy; the phenol red–free formulation avoids phenol red absorbance interference in optical assays.
Rich, stable nutrient profile
32 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.
Customization on demand
pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge from premature fouling.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and organisms in the mycoplasma size range (0.2–0.3 µm) that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
A second, dedicated 0.1 µm prefilter protecting the second 0.04 µm cartridge — full redundancy against upstream bypass.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-DMEMH-PR1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMH-PR1X combines high-glucose energy support with supplemental HEPES buffering — supporting platforms from open-top microfluidic chips to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
HEPES buffering supports pH stability during automated bioreactor perfusion outside continuous CO₂ control. An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for robotic liquid handlers where trace particulates cause valve failure.
- Reduced Particulate Load: Quadruple-stage 0.04 µm filtration reduces particulate carryover in bioreactor media lines
- pH-Stable Automated Delivery: HEPES helps maintain pH during robotic media exchanges outside a CO₂ incubator
- Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Open-Top & Variable-CO₂ Chips
Supplemental HEPES buffering supports pH-stable culture in open-top microfluidic devices and multi-compartment chips with heterogeneous CO₂ environments.
Primary Neurons & Brain-on-Chip
High glucose (4.5 g/L) plus HEPES buffering supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion.
Cardiomyocyte & Heart-on-Chip
High-glucose DMEM plus HEPES is a common base for iPSC-CM maturation and heart-on-chip functional assays requiring stable pH and a high-energy substrate.
Warburg Effect & Cancer Models
High glucose supports aerobic glycolysis in cancer lines; HEPES buffering helps stabilize pH during rapid glucose consumption in Warburg-active tumor models.
Metabolic Flux Analysis
Defined high-glucose formulation supports ¹³C isotope tracing and NMR-based metabolic flux analysis. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium; this formulation retains sodium bicarbonate buffering.
Microscopy & Optical Sensing
HEPES-supported pH stability during imaging sessions; the phenol red–free formulation reduces background absorbance for confocal microscopy and biosensor platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] Sodium Pyruvate, [-] Phenol Red |
| Appearance | Colorless to pale yellow, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H2O |
| Total ingredients | 32 (4 formulation categories, 3 composition tabs) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release spec) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Controlled by 0.1 & 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | HEPES-buffered (25 mM); supports 5% CO₂ incubation via the retained bicarbonate system, while HEPES enables handling outside a CO₂ incubator — validate for your specific application. |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 32 ingredients verified per lot with CAS numbers for raw-material traceability. HEPES (25 mM = 5958 mg/L) and D-Glucose are listed under OTHERS.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 4500.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QA and testing at the Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity minimizes trace-metal and organic-carbon contamination in raw-material water.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every batch — no blending, no averaged QC results.
Endotoxin — USP <85> BET
LAL assay; batch release specification < 0.05 EU/mL; assay sensitivity 0.005 EU/mL.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 310–350 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every batch on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMH-PR1X compares
FluxMPS™ DCP-DMEMH-PR1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMH-PR1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG + HEPES (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | N/A (standard grade) | N/A (standard grade) |
| HEPES-buffered High Glucose DMEM — no Pyruvate and no Phenol Red for imaging-clean carbon control | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 & 0.04 µm) | cancel No | cancel No |
| HEPES buffer (25 mM) | check_circle Yes | cancel No | check_circle Yes |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMH-PR1X DMEM High Glucose + 25mM HEPES.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
