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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

Product#: DCP-DMEMH-P1X
$44.00
DCP-DMEMH-P1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Without Sodium Pyruvate

FluxMPS™ DCP-DMEMH-P1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and open-top microfluidic platforms. Sodium pyruvate is omitted so researchers can add it fresh for precise metabolic control. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • High Glucose (4.5 g/L) formulation supports energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, and aerobically active cancer lines — in long-duration microfluidic perfusion
  • 25 mM HEPES (pKa 7.3 at 37°C) buffers pH during handling outside the incubator, alongside the 3700 mg/L sodium bicarbonate system used for closed-incubator culture
  • Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaches a 0.04 µm final cut-off for microfluidic channel compatibility
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • Sodium pyruvate omitted from the formulation for researcher-controlled fresh addition at time of use
  • Manufactured under an ISO 13485:2016 quality management system; final QC testing at Diagnocine's Totowa, NJ facility
  • Custom pH, glucose concentration, HEPES level, salts, and nutrient adjustments available on request
CAT. NO.
DCP-DMEMH-P1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Sodium Pyruvate
  • AppearanceRed-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized organisms, subvisible particulates, and fine debris that accumulate in microfluidic channels. Standard DMEM also lacks HEPES buffering, causing pH instability whenever chips are handled outside incubators. FluxMPS™ addresses both failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance supports safe perfusion in microchannel geometries below 100 µm.

bolt

High-energy cell support

4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.

water_drop

HEPES: supplemental pH stability

25 mM HEPES (pKa 7.3 at 37°C) provides additional buffering during handling outside CO2 control. This formulation retains sodium bicarbonate, so standard closed-incubator culture still requires a CO2 incubator (see Technical Specifications).

visibility

Low background for imaging

Quadruple-stage filtration maintains an ultra-low particulate baseline suited to confocal and biosensor platforms on chip. This formulation contains phenol red as a pH indicator; contact us for a phenol-red-free variant where minimal optical background is required.

science

Rich, stable nutrient profile

33 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.

tune

Customization on demand

pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated 0.1 µm prefilter + 0.04 µm final-filter pairs — reaching a validated 0.04 µm final cut-off for microfluidic channel compatibility.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris and protein clumps; protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — four validated filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved via 0.1 µm mycoplasma-retentive membrane filtration (not tested per lot); mycoplasma organisms measure 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEMH-P1X Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable DMEM High Glucose HEPES for organ-on-a-chip and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off.
© Diagnocine® — DCP-DMEMH-P1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEMH-P1X combines high-glucose energy support with HEPES pH stability — supporting platforms from open-top microfluidic chips to multi-organ body-on-a-chip systems.

Automated Bioreactors & Robotics

Next-Generation System Uptime

HEPES buffering provides supplemental pH stability during automated bioreactor perfusion and brief periods outside CO2 control. An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for robotic liquid handlers where trace particulates cause valve failure.

  • Total Particulate Exclusion: The 10 nm variant removes nanoparticulate aggregates from bioreactor media lines
  • pH-Stable Automated Delivery: HEPES supports pH during brief robotic media exchanges outside CO2 control
  • Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Open-Top & Microfluidic Chips

HEPES buffering supports pH-stable culture in open-top microfluidic devices and multi-compartment chips with heterogeneous CO2 environments.

OoCToCBoCLoCMPS
Neuroscience

Primary Neurons & Brain-on-Chip

High glucose (4.5 g/L) plus HEPES stability supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion.

iPSC-NeuronsPrimary neuronsBrain-on-chip
Cardiac Biology

Cardiomyocyte & Heart-on-Chip

High-glucose DMEM plus HEPES is a standard base for iPSC-CM maturation and heart-on-chip functional assays requiring high energy substrate.

iPSC-CMHeart-on-chipTEER
Cancer Biology

Warburg Effect & Cancer Models

High glucose supports aerobic glycolysis in cancer lines; HEPES provides supplemental buffering during rapid glucose consumption spikes in Warburg-active tumour models.

MCF-7MDA-MB-231HeLaA549
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation for 13C isotope tracing and NMR metabolomics; HEPES allows sampling outside incubators without pH artefacts. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

HEPES-stabilized pH during imaging sessions outside the incubator. This formulation contains phenol red as a pH indicator; contact us for a phenol-red-free variant when minimal optical background is required.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Sodium Pyruvate
Appearance Red-colored, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Total ingredients 33
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Approximately 10% CO2 recommended for closed-incubator culture (sodium bicarbonate 44 mM / 3700 mg/L system targeting pH 7.4); 25 mM HEPES provides supplemental buffering during handling outside CO2 control.
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Pack sizes 500 mL, 1000 mL
Formulation

Full composition (mg/L)

DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 33 ingredients verified per lot with CAS numbers for raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the OTHERS group.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
i-Inositol 87-89-8 7.200
OTHERS
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.900
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-DMEMH-P1X custom specifications — pH, glucose, HEPES concentration, salts, or nutrient modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA and testing at Diagnocine's R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm resistivity supports trace-metal and organic-carbon (TOC) control for sensitive cell culture applications.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.

assignment

Micro-Batch Precision

Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 310–350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-DMEMH-P1X lot at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-P1X compares

FluxMPS™ DCP-DMEMH-P1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEMH-P1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard DMEM HG + HEPES
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm final cut-off) Standard Standard
HEPES-buffered High Glucose DMEM — no Sodium Pyruvate for precise metabolic control check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stage) cancel No cancel No
HEPES buffer (25 mM) check_circle Yes cancel No check_circle Yes
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-P1X DMEM High Glucose + 25mM HEPES.

Yes. DCP-DMEMH-P1X combines a validated quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) for ultra-low particulate delivery in microfluidic channels with 25 mM HEPES buffering for additional pH stability outside CO2 control. High glucose (4.5 g/L) supports energy-demanding cell types — neurons, cardiomyocytes, and cancer lines — in long-duration chip perfusion. Because sodium bicarbonate (3700 mg/L) is retained in this formulation, standard closed-incubator culture still requires approximately 10% CO2 to hold pH 7.4; HEPES is most valuable during handling outside the incubator or in open-top chip designs.
 
Sodium pyruvate is omitted so researchers can add it fresh (typically 1 mM / 110 mg/L) at time of use, enabling precise control of oxidative metabolism support. This formulation retains both sodium bicarbonate (3700 mg/L) and HEPES (25 mM) for dual-buffer pH stability during and after supplementation.
Because this formulation contains sodium bicarbonate at 44 mM (3700 mg/L), a standard CO2 incubator set to approximately 10% CO2 is required to hold pH at the target 7.4 in closed culture systems. The added 25 mM HEPES (pKa 7.3 at 37°C) provides supplemental buffering capacity during brief periods outside CO2 control — bench handling, imaging, or open-top chip designs — but does not eliminate the need for CO2 in standard closed incubation.
Yes. Add FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or custom nutrients as required. When adding serum or other protein-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF filter (0.1 µm filtration is suitable only for defined, protein-free additions). Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before release and must meet the release specification of < 0.05 EU/mL. A Certificate of Analysis is available on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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