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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid

Product#: DCP-DMEMH-QR1X
$71.50
DCP-DMEMH-QR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Phenol Red

FluxMPS™ DCP-DMEMH-QR1X is a Microfluidics Suitable, ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and open-top or CO2-tolerant microfluidic platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides supplemental pH buffering alongside the sodium bicarbonate primary buffer system. Formulation: [+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L), [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red.

  • High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and other aerobically active cell types
  • 25 mM HEPES (pKa 7.3 at 37°C) provides supplemental pH buffering that improves tolerance to CO2 fluctuation, alongside the sodium bicarbonate primary buffer
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish for microfluidic channels below 100 µm
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Formulated without L-Glutamine (add fresh at time of use) and without Phenol Red for reduced background fluorescence in imaging assays
  • Manufactured under an ISO 13485:2016 quality management system with per-lot Certificate of Analysis
  • Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request
CAT. NO.
DCP-DMEMH-QR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red
  • AppearancePale yellow to colorless, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310 - 350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
Available sizes: 500 mL, 1000 mL
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized organisms, subvisible particulates, and endotoxin-bearing fragments that accumulate in microfluidic channels. Standard DMEM High Glucose also relies on bicarbonate alone for pH control, which drifts whenever chips are handled outside a CO2 incubator. FluxMPS™ addresses both failure modes simultaneously.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in chip geometries, including sub-100 µm channels.

bolt

High-energy cell support

4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.

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HEPES: Supplemental pH Buffering

25 mM HEPES (pKa 7.3 at 37°C) supplements the bicarbonate buffer system, improving tolerance to CO2 fluctuation during handling outside the incubator — useful for open-top chips and imaging workflows.

visibility

Low background for imaging

0.04 µm final filtration delivers an ultra-low particulate baseline, and the phenol red–free formulation reduces optical interference for confocal microscopy and biosensor platforms on chip.

science

Rich, stable nutrient profile

33 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.

tune

Customization on demand

pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions — delivering mycoplasma-retentive purity beyond what conventional 0.22 µm media achieve.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge downstream.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates, including mycoplasma-sized organisms (0.2–0.3 µm), that a standard 0.22 µm filter does not.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — mycoplasma-retentive polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved via 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEMH-QR1X Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid ? Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish ? Microfluidics Suitable DMEM High Glucose HEPES for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for mycoplasma-retentive purity.
© Diagnocine® — DCP-DMEMH-QR1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEMH-QR1X combines high-glucose energy support with HEPES-supplemented pH buffering — supporting demanding platforms from open-top microfluidic chips to multi-organ body-on-a-chip systems.

Automated Bioreactors & Robotics

Next-Generation System Uptime

HEPES buffering improves pH tolerance during automated bioreactor perfusion, reducing sensitivity to transient CO2 fluctuations. An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for robotic liquid handlers where even trace particulates cause valve failure.

  • Total Particulate Exclusion: the optional 10 nm variant removes nanoparticulate aggregates from bioreactor media lines
  • Improved Buffering During Automated Delivery: HEPES supplements pH stability during robotic media exchanges, reducing CO2 re-equilibration delays
  • Extended Perfusion Stability: consistent high-glucose delivery with supplemented pH stability over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Open-Top & CO2-Tolerant Chips

HEPES supplemental buffering supports pH stability in open-top microfluidic devices, atmospheric incubators, and multi-compartment chips with heterogeneous CO2 environments.

OoCToCBoCLoCMPS
Neuroscience

Primary Neurons & Brain-on-Chip

High glucose (4.5 g/L) plus HEPES-supplemented buffering supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion.

iPSC-NeuronsPrimary neuronsBrain-on-chip
Cardiac Biology

Cardiomyocyte & Heart-on-Chip

High-glucose DMEM with HEPES supplementation is a standard base for iPSC-CM maturation and heart-on-chip functional assays requiring stable pH and high energy substrate.

iPSC-CMHeart-on-chipTEER
Cancer Biology

Warburg Effect & Cancer Models

High glucose supports aerobic glycolysis in cancer lines; HEPES supplementation helps stabilize pH during rapid glucose consumption spikes in Warburg-active tumor models.

MCF-7MDA-MB-231HeLaA549
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation for ¹³C isotope tracing and NMR metabolomics; HEPES allows sampling outside incubators without pH artifacts. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

HEPES-supplemented pH stability during imaging sessions; the phenol red–free formulation reduces background fluorescence for confocal and biosensor platforms.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate (3700 mg/L), [+] 25mM HEPES (5958 mg/L), [+] Calcium (265 mg/L CaCl2·2H2O), [+] Magnesium (97.72 mg/L MgSO4), [+] High Glucose (4500 mg/L), [+] Sodium Pyruvate (110 mg/L) | [-] L-Glutamine, [-] Phenol Red
Appearance Pale yellow to colorless, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
L-Glutamine Not added — supplement fresh at time of use
Sodium Pyruvate 110 mg/L
Phenol Red Not added
pH USP <791> 7.4
Osmolality USP <785> 310 - 350 mOsm/kg H2O
Total ingredients 33
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (release specification, per batch)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Approximately 10% CO2 (sodium bicarbonate 3700 mg/L primary buffer); 25 mM HEPES provides supplemental buffering for CO2-free handling
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 33 ingredients verified per lot with CAS numbers for full raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the OTHERS group.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.00
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 584.000
L-Isoleucine 73-32-5 42.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 105.000
L-Methionine 63-68-3 146.000
L-Phenylalanine 63-91-2 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 4500.000
Sodium pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-DMEMH-QR1X custom specifications — pH, glucose, HEPES concentration, salts, or nutrient modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA and testing at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

Type 1 water, 18.2 MΩ·cm resistivity, with controlled trace-metal and total organic carbon (TOC) content for consistent raw-material quality.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, ensuring container-closure integrity.

assignment

Micro-Batch Precision

Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 310 - 350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-DMEMH-QR1X lot at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-QR1X compares

FluxMPS™ DCP-DMEMH-QR1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEMH-QR1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard DMEM HG + HEPES
(0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
HEPES-buffered High Glucose DMEM — no L-Glutamine, no Phenol Red check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 & 0.04 µm stages) cancel No cancel No
HEPES buffer (25 mM) check_circle Yes cancel No check_circle Yes
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-QR1X DMEM High Glucose + 25mM HEPES.

Yes. DCP-DMEMH-QR1X is a Microfluidics Suitable formulation combining Quadruple-stage 0.04 µm filtration for ultra-low particulate delivery in microfluidic channels with 25 mM HEPES buffering for supplemental pH stability. High glucose (4.5 g/L) supports energy-demanding cell types — neurons, cardiomyocytes, and cancer lines — in long-duration chip perfusion. The HEPES component is particularly useful for open-top chips where CO2 concentration fluctuates.
FluxMPS™ uses four sequential filtration passes — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish) — reaching a 0.04 µm final cut-off versus the single 0.22 µm pass used in conventional media. This delivers approximately 5× lower particulate counts and mycoplasma-retentive filtration at the 0.1 µm and 0.04 µm stages.
Phenol red is excluded to reduce background fluorescence for live-cell imaging and hormone-sensitive assays. L-glutamine is excluded because it degrades in liquid storage; add it fresh at time of use according to your cell line's requirements. HEPES plus sodium bicarbonate provides dual-buffer pH support in the meantime.
This formulation contains sodium bicarbonate (3700 mg/L) as its primary buffer, which is formulated for approximately 10% CO2 incubation to hold pH 7.4. The additional 25 mM HEPES (pKa 7.3 at 37°C) provides supplemental pH stability during CO2-free handling — for example, during microfluidic loading, imaging outside the incubator, or open-top chip operation — but does not eliminate the need for CO2 incubation during routine culture.
Yes. Add fetal bovine serum (FBS, typically 5–10%), serum-free supplements, growth factors, or antibiotics as required for your application. Filter serum-containing or protein-containing additions through a low-protein-binding 0.2 µm PES or PVDF membrane before adding to the medium; do not use a 0.04 µm membrane for supplements, as it will retain serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) before release and must meet the release specification of < 0.05 EU/mL. A Certificate of Analysis reporting the batch result is available on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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