FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES: 1X Liquid
FluxMPS™ DCP-DMEMH1X is a Microfluidics Suitable, quadruple-stage ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and applications requiring stable pH during brief CO₂-free handling on microfluidic platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it targets ultra-low particulate delivery for sub-100 µm microchannel geometries. HEPES (25 mM, pKa 7.3 at 37°C) supplements the bicarbonate buffer system for added pH stability outside the incubator.
- High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and aerobically active cells
- 25 mM HEPES (pKa 7.3 at 37°C) — supplemental pH buffering for handling outside CO₂ incubation
- 0.04 µm final nano-filtration — engineered for sub-100 µm microfluidic channel geometries
- Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- Ultrapure Type 1 water (18.2 MΩ·cm); manufactured under an ISO 13485:2016 quality management system with ISO Class 5 aseptic fill
- Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (4.5 g/L), [+] Sodium Pyruvate
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)320–360 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma-scale particulates and subvisible aggregates that accumulate in microfluidic channels. Standard DMEM also relies on bicarbonate buffering alone, which drifts in pH the moment a chip is handled outside a CO₂ incubator. FluxMPS™ addresses both failure modes.
Microchannel-safe purity
0.04 µm final filtration through a validated quadruple-stage train, engineered for safe perfusion in sub-100 µm chip geometries.
High-energy cell support
4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.
HEPES: supplemental pH stability
25 mM HEPES (pKa 7.3 at 37°C) helps maintain pH near 7.4 during brief handling outside CO₂ incubation — useful for open-top chips and point-of-care sampling.
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy and biosensor platforms on chip; a phenol red–free formulation is available on request for further reduction of optical background.
Rich, stable nutrient profile
34 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.
Customization on demand
pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish, engineered for ultra-low particulate delivery unavailable from conventional 0.22 µm media.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ DCP-DMEMH1X is processed through a validated quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2), with HEPES buffering for supplemental pH stability and mycoplasma-retentive filtration engineered into every production stage.
© Diagnocine® — DCP-DMEMH1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMH1X combines high-glucose energy support with HEPES-supplemented pH stability — supporting platforms from open-top microfluidic chips to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
HEPES buffering provides supplemental pH stability during automated bioreactor perfusion and media exchanges. An optional 0.01 µm (10 nm) MPS Grade variant is available on request for robotic liquid handlers where trace particulates cause valve failure.
- Extended Particulate Control: Quadruple-stage 0.04 µm filtration reduces particulate load in bioreactor media lines
- HEPES-Supported Delivery: Supplemental pH stability during robotic media exchanges outside CO₂ incubation
- Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Open-Top Chips & Microphysiological Systems
HEPES supplementation supports pH-stable handling in open-top microfluidic devices and multi-compartment chips with heterogeneous CO₂ environments.
Primary Neurons & Brain-on-Chip
High glucose (4.5 g/L) supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion.
Cardiomyocyte & Heart-on-Chip
High-glucose DMEM with HEPES is a standard base for iPSC-CM maturation and heart-on-chip functional assays requiring high energy substrate.
Warburg Effect & Cancer Models
High glucose supports aerobic glycolysis in cancer lines; HEPES supplementation helps stabilize pH during rapid glucose consumption spikes.
Metabolic Flux Analysis
Defined high-glucose formulation supports ¹³C isotope tracing and NMR metabolomics for flux analysis in Warburg-active and aerobically respiring cell models. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate baseline supports confocal and biosensor platforms; a phenol red-free formulation is available on request to further reduce optical background.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (4.5 g/L), [+] Sodium Pyruvate |
| Appearance | Red-colored, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | 110 mg/L |
| Phenol Red | 15.9 mg/L (phenol red sodium salt) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 320–360 mOsm/kg H2O |
| Total ingredients | 34 across 4 categories |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ recommended (bicarbonate-buffered base); 25 mM HEPES provides supplemental pH stability for CO₂-free handling outside the incubator |
| Available pack sizes | 500 mL, 1000 mL |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 34 ingredients verified per lot with CAS numbers for full raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the OTHERS group.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016–certified quality management system. Final QA and testing at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity Type 1 water; trace-metal and organic-carbon (TOC) controlled to minimize feed-water contaminant carryover.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 320–360 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMH1X compares
FluxMPS™ DCP-DMEMH1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMH1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG + HEPES (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm) | Not applicable (0.22 µm filtered) | Not applicable (0.22 µm filtered) |
| Dual-buffered: 25 mM HEPES plus Sodium Bicarbonate | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMH1X DMEM High Glucose + 25mM HEPES.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-supplemented, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
