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- FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/F-12 (3:1) medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under an ISO 13485:2016 quality management system, with final QC and customization at Diagnocine, Totowa, NJ. This formulation omits L-glutamine, sodium bicarbonate, and calcium chloride so research groups can build their own defined buffering and calcium strategy.
- Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- 3.8255 g/L D-glucose and 27.525 mg/L sodium pyruvate retained as the carbon-source backbone
- Formulated without L-glutamine, sodium bicarbonate, and calcium chloride — add back to match your own culture protocol
- Bicarbonate-free base; CO2-independent buffering (e.g., HEPES) to be supplied by the end user
- Manufactured under an ISO 13485:2016 quality management system; final QC and micro-batch customization at Diagnocine, Totowa, NJ, USA
- pH, glucose concentration, salts, HEPES, and nutrient composition available on request — support@diagnocine.com
- Glucose3.8255 g/L ([+])
- L-GlutamineNot added ([-])
- Sodium Pyruvate27.525 mg/L ([+])
- pH (USP <791>)7.4
- Osmolality (USP <785>)260 - 300 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and interfering with metabolic assays. FluxMPS™ closes that gap with a validated multi-stage filtration train.
Microchannel-safe purity
0.04 µm final filtration and USP <788> Method 1 (light obscuration) particulate testing support unobstructed flow through narrow microfluidic geometries.
Defined carbon-source backbone
3.8255 g/L D-glucose and 27.525 mg/L sodium pyruvate are held at fixed, documented concentrations to support Warburg-effect studies and glycolysis-focused metabolic work.
Controlled water quality
Prepared with trace-metal and organic-carbon controlled process water as part of the multi-stage filtration workflow.
Low background for imaging
A low particulate baseline supports confocal live-cell imaging and biosensor readouts; note that phenol red and riboflavin in this formulation contribute their own optical background and should be accounted for in fluorescence and absorbance assay design.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch production for tight lot-to-lot consistency in long-duration MPS perfusion.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ DCP-DMF12-QBCT1X is manufactured through a validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — well below the 0.22 µm cut-off used by conventional cell culture media.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates, protecting the first 0.04 µm final filter cartridge.
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2
0.04 µm Final filtration I
Retains sub-micron particulates and microaggregates that pass through standard 0.22 µm filtration.
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3
0.1 µm Prefiltration II
A second, dedicated prefilter protects the second 0.04 µm final filter cartridge, giving the train full redundancy.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter prior to aseptic fill and finish.
Performance vs. conventional media
The quadruple-stage, paired prefilter-plus-final-filter train reaches a 0.04 µm final cut-off, well below the 0.22 µm pore size used in conventional cell culture media manufacturing.
© Diagnocine® — DCP-DMF12-QBCT1X
Optimized for next-generation cell biology platforms
FluxMPS™ DCP-DMF12-QBCT1X is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.
Automated Bioreactors & Robotics
For automated perfusion bioreactors and robotic liquid-handling platforms, Diagnocine also offers this formulation in an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant — a separate product line from the 0.04 µm Microfluidics Suitable medium described on this page.
- Total particulate exclusion at the sub-40 nm level for long-run automated systems
- Reduced risk to valves, sensors, and microfluidic interconnects from residual particulates
- Extended perfusion stability for multi-week, unattended bioreactor runs
Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request specifications and lead time.
Micro Physiological System (MPS) & Chip
Ultra-filtered media for perfusion in organ chips, tissue chips, and body-on-a-chip devices where microchannel occlusion must be avoided.
Warburg Effect & Metabolic Research
Fixed-concentration glucose (3.8255 g/L) and sodium pyruvate (27.525 mg/L) support Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.
iPSC-Derived Models
Low-particulate, batch-tested baseline for iPSC differentiation and functional organoid readouts; supplement with L-glutamine and calcium per your protocol.
Endothelial & Primary Cells
Particulate-controlled perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture; calcium supplementation required per assay design.
Metabolic Flux Analysis
Chemically defined carbon-source concentrations support ¹³C metabolic tracing and NMR metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium; this formulation retains phenol red.
Microscopy & Optical Sensing
Low particulate baseline for confocal microscopy and biosensor-based readouts on chip; account for phenol red and riboflavin background in fluorescence assay design.
Full technical specification
Every lot of FluxMPS™ DCP-DMF12-QBCT1X is released against multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] Phenol Red, Magnesium, 3.8255 g/L Glucose, 27.525 mg/L Sodium Pyruvate / [-] L-Glutamine, Sodium Bicarbonate, Calcium Chloride |
| Appearance | Clear, pink-to-red solution (phenol red pH indicator present) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 260 - 300 mOsm/kg H2O |
| Glucose | 3.8255 g/L ([+]) |
| L-Glutamine | Not added ([-]) |
| Sodium Pyruvate | 27.525 mg/L ([+]) |
| Phenol Red | 12.235 mg/L ([+]) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL (batch release) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm <788> M1 | Tested per lot; results on CoA |
| Particulate ≥25 µm <788> M1 | Tested per lot; results on CoA |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO2 requirement | Bicarbonate-free formulation; CO2-independent buffering (e.g., HEPES) to be supplied by end user |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 48 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| Copper sulfate pentahydrate | 7758-99-8 | 0.000625 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 35.510 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.075 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.209 |
| Magnesium chloride hexahydrate | 7791-18-6 | 14.412 |
| Magnesium sulfate anhydrous | 7487-88-9 | 73.290 |
| Potassium chloride | 7447-40-7 | 356.000 |
| Sodium chloride | 7647-14-5 | 6699.750 |
| Sodium dihydrogen phosphate monohydrate | 10049-21-5 | 81.750 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.215 |
| Component | CAS Number | mg/L |
|---|---|---|
| Glycine | 56-40-6 | 24.380 |
| L-Alanine | 56-41-7 | 2.227 |
| L-Arginine hydrochloride | 1119-34-2 | 115.520 |
| L-Asparagine monohydrate | 5794-13-8 | 3.752 |
| L-Aspartic acid | 56-84-8 | 3.325 |
| L-Cysteine dihydrochloride | 7048-04-6 | 8.780 |
| L-Cystine hydrochloride monohydrate | 46.927 | |
| L-Glutamic acid | 56-86-0 | 3.675 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 36.740 |
| L-Isoleucine | 73-32-5 | 79.735 |
| L-Leucine | 61-90-5 | 82.025 |
| L-Lysine hydrochloride | 657-27-2 | 118.625 |
| L-Methionine | 63-68-3 | 23.625 |
| L-Phenylalanine | 63-91-2 | 50.740 |
| L-Proline | 147-85-3 | 8.625 |
| L-Serine | 56-45-1 | 34.125 |
| L-Threonine | 72-19-5 | 74.225 |
| L-Tryptophan | 73-22-3 | 12.510 |
| L-Tyrosine disodium salt | 79.795 | |
| L-Valine | 72-18-4 | 73.425 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 6.490 |
| D-Biotin | 58-85-5 | 0.001825 |
| D-Ca-Pantothenate | 137-08-6 | 3.120 |
| Folic acid | 59-30-3 | 3.330 |
| Nicotinamide | 98-92-0 | 3.000 |
| Pyridoxine hydrochloride | 58-56-0 | 3.015 |
| Riboflavin | 83-88-5 | 0.309 |
| Thiamine hydrochloride | 67-03-8 | 3.085 |
| Vitamin B12 | 68-19-9 | 0.340 |
| OTHERS | ||
| myo-Inositol | 87-89-8 | 9.900 |
| D-Glucose | 50-99-7 | 3825.500 |
| DL-Thioctic acid | 1200-22-2 | 0.053 |
| Hypoxanthine | 68-94-0 | 1.200 |
| Linoleic acid | 60-33-3 | 0.021 |
| Phenol red sodium salt | 34487-61-1 | 12.235 |
| Putrescine hydrochloride | 333-93-7 | 0.040 |
| Sodium pyruvate | 113-24-6 | 27.525 |
| Thymidine | 50-89-5 | 0.183 |
Manufacturing & compliance
Every batch is subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA, and testing at the Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Controlled Water Quality
Ultrapure Type 1 process water (18.2 MΩ·cm) with trace-metal and organic-carbon control, used throughout the qualified manufacturing process.
Quadruple-Stage Filtration
0.1 µm ×2 + 0.04 µm ×2 filtration train with aseptic fill and finish in an ISO Class 5 (Class 100) environment.
Micro-Batch Precision
Small-batch production for tight lot-to-lot consistency, supporting reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay method; assay sensitivity 0.005 EU/mL. Batch release specification: < 0.05 EU/mL. See batch quality control note below.
Particulate — USP <788> Method 1
Light obscuration particle count testing performed per lot; results reported on the CoA.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 260 - 300 mOsm/kg H2O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMF12-QBCT1X compares
Filtration architecture, formulation definition, and QC depth compared against conventional 0.22 µm filtered DMEM/F-12 media and published competitor specifications.
| Parameter | DCP-DMF12-QBCT1X (FluxMPS™) | Other cell culture media suppliers |
|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm final cut-off) | Standard grade (0.22 µm filtered) |
| Formulation | [+] Phenol Red, Magnesium, 3.8255 g/L Glucose, 27.525 mg/L Sodium Pyruvate / [-] L-Glutamine, Sodium Bicarbonate, Calcium Chloride | Varies by supplier |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm (typical) |
| Number of filtration stages | 4 (Quadruple) | 1 (typical) |
| Mycoplasma-retentive filtration | check_circle | cancel (typical) |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
| USP <788> particulate testing | Method 1 (light obscuration), tested per lot | Varies |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified (typical) |
| Manufacturing QMS | ISO 13485:2016 | Varies |
| Microfluidic channel compatibility | check_circle | cancel (typical) |
| Custom formulation | check_circle On request | cancel (typical) |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMF12-QBCT1X.
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
