FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/F-12 (3:1) engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). This glutamine-free, bicarbonate-free formulation supports CO₂-independent or custom-buffered culture.

• Quadruple-stage filtration to a 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2) for microchannel-safe, sub-mycoplasma polishing
• Endotoxin release specification: less than 0.05 EU/mL by USP <85> LAL assay, tested per manufacturing batch
• Glutamine-free, bicarbonate-free formulation with 3.8255 g/L glucose and 27.525 mg/L sodium pyruvate for CO₂-independent or custom-buffered culture
• Phenol red present (12.235 mg/L) for visual pH monitoring; ultra-low particulate baseline supports confocal and biosensor imaging
• 4× concentrated BME amino acid and vitamin profile for extended microphysiological system (MPS) perfusion stability
• Manufactured under an ISO 13485:2016 quality management system with a per-batch Certificate of Analysis
• Custom pH, glucose, HEPES, salts & nutrient adjustments available on request — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaches a final pore size of 0.04 µm — two dedicated prefilter + final-filter pairs run in series, capturing particulates and mycoplasma-scale organisms that 0.22 µm filtration misses.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm cartridge and preserves microchannel chip geometries.

2. 2

   0.04 µm Final filtration I

   Retains fine particulates and mycoplasma-scale organisms (typical diameter 0.2–0.3 µm) largely absent from standard 0.22 µm media.

3. 3

   0.1 µm Prefiltration II

   A second, dedicated prefilter protecting the second 0.04 µm cartridge — redundancy for the sterile-class production run.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ DMEM/F-12 (3:1) w/o L-Glutamine, Sodium Bicarbonate is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

For automated perfusion bioreactors and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available — the separate six-stage cascade line, distinct from the Microfluidics Suitable 0.04 µm product described on this page.

• Total Particulate Exclusion — minimizes valve and micro-orifice fouling in long-run automated systems
• Valve & Sensor Protection — reduces particulate load on inline optical and electrochemical sensors
• Extended Perfusion Stability — supports multi-week unattended perfusion runs

Inquiry Required: the 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request specifications and lead time.

Full technical specification

Every lot of FluxMPS™ DMEM/F-12 (3:1) w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is released against multi-parameter QC specifications.

Full composition (mg/L)

This 1X liquid formulation excludes L-Glutamine and Sodium Bicarbonate. Total: 49 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch is subjected to multi-parameter release testing before distribution.

How DCP-DMF12-QBT1X compares

Filtration, mycoplasma barrier, water quality, and QC depth compared with conventional 0.22 µm filtered media for MPS and microfluidic applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DMEM/F-12 (3:1) w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid (DCP-DMF12-QBT1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
9. Ham RG. Nutritional requirements of primary cultures. In Vitro Cell Dev Biol. 1984;20:275–290. doi:10.1007/BF02618531