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- FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/Ham's F-12 (3:1) medium, bicarbonate-free and buffered with 15 mM HEPES, engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 using Ultrapure Type 1 water (18.2 MΩ·cm).
- Bicarbonate-free, 15 mM HEPES-buffered formulation for reduced CO2-dependent or CO2-independent incubation
- Quadruple-stage filtration to a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2)
- Endotoxin release specification < 0.05 EU/mL (USP <85> BET, tested per manufacturing batch)
- Contains 3.8255 g/L D-glucose, 474.5 mg/L L-glutamine and 27.525 mg/L sodium pyruvate for standard glycolytic and OXPHOS metabolism
- Phenol red present (12.235 mg/L) as a visible pH indicator; not suited to phenol-red-free optical or fluorescence assays
- 3:1 DMEM/Ham's F-12 base with 4× BME amino acid and vitamin concentrations
- Manufactured under an ISO 13485:2016 quality management system with per-batch release testing and Certificate of Analysis
- Custom pH, glucose, HEPES, salts and nutrient composition available on request — support@diagnocine.com
- Glucose3.8255 g/L
- L-Glutamine474.5 mg/L ([+])
- Sodium Pyruvate27.525 mg/L ([+])
- HEPES15 mM ([+])
- Osmolality (USP <785>)260 - 300 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from manufacture, unopened
- ShippingCold pack (2-8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm) and sub-visible particulates to pass freely — clogging microchannels and complicating long-duration culture. FluxMPS™ closes that gap with a validated 0.04 µm final filtration.
Microchannel-safe purity
A 0.04 µm final filter reduces particulate load relative to standard 0.22 µm media, supporting unobstructed flow in narrow microfluidic channels.
Total metabolic control
Defined glucose, glutamine and pyruvate levels support Warburg-effect studies, glycolysis/OXPHOS flux analysis, and ¹³C metabolic tracing.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon control, reducing an important source of lot-to-lot variability.
Low background for imaging
Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip. This formulation contains phenol red and riboflavin; consider a phenol-red-free variant for assays sensitive to background absorbance or autofluorescence.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch precision — consistent lot-to-lot performance for long-duration culture.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
DCP-DMF12H-BT1X is manufactured through a validated four-stage sequential filtration train reaching a final pore size of 0.04 µm, engineered for microfluidic channels and organ-on-a-chip (OoC) applications.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter cartridge.
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2
0.04 µm Final filtration I
Retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
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3
0.1 µm Prefiltration II
Second dedicated prefilter protecting the second 0.04 µm final filter cartridge.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill and finish in an ISO Class 5 (Class 100) laminar-flow workstation.
Filtration architecture
The train runs as two redundant prefilter/final-filter pairs in series — each 0.04 µm final filter is protected by its own dedicated 0.1 µm prefilter, rather than a single descending cascade.
© Diagnocine® — DCP-DMF12H-BT1X
Optimized for next-generation cell biology platforms
FluxMPS™ DCP-DMF12H-BT1X is suited to applications where microchannel cleanliness and defined metabolic composition are important.
Automated Bioreactors & Robotics
For automated bioreactor and robotic liquid-handling platforms, Diagnocine also offers a 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, engineered to further reduce particulate load in long-duration, unattended perfusion systems.
- Total Particulate Exclusion: 0.01 µm final cut-off for maximal protection of narrow-bore tubing and valves
- Valve & Sensor Protection: minimizes particulate fouling of optical and electrochemical in-line sensors
- Extended Perfusion Stability: supports longer unattended run times between filter changes
Inquiry Required: the 0.01 µm MPS Grade variant is available by request — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultra-filtered media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Defined glucose, glutamine and pyruvate composition for Warburg-effect studies and aerobic glycolysis research.
iPSC-Derived Models
Low-particulate baseline supports iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Low-particulate perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined glucose, glutamine and pyruvate levels support ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium; this formulation, while bicarbonate-free, contains phenol red.
Microscopy & Optical Sensing
Low particulate baseline supports confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Full technical specification
Every batch of FluxMPS™ DCP-DMF12H-BT1X is released against multi-parameter QC specifications. Available pack sizes: 500 mL, 1000 mL.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, Phenol Red, 15mM HEPES, Calcium, Magnesium, 3.8255 g/L Glucose, Sodium Pyruvate / [-] Sodium Bicarbonate |
| Appearance | Clear, phenol red-colored solution |
| Osmolality USP <785> | 260 - 300 mOsm/kg |
| Glucose | 3.8255 g/L (3825.500 mg/L) |
| L-Glutamine | 474.5 mg/L ([+]) |
| Sodium Pyruvate | 27.525 mg/L ([+]) |
| Phenol Red | 12.235 mg/L ([+]) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL (batch release specification) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2-8°C) |
| CO2 requirement | HEPES-buffered; reduced CO2 dependence (validate per cell line) |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Traceability | Full raw-material and lot traceability per batch |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 51 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
INORGANIC SALTS
| Component | CAS Number | mg/L |
|---|---|---|
| Calcium chloride dihydrate | 10035-04-8 | 209.775 |
| Copper sulfate pentahydrate | 7758-99-8 | 0.000625 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 35.510 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.075 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.209 |
| Magnesium chloride hexahydrate | 7791-18-6 | 14.412 |
| Magnesium sulfate anhydrous | 7487-88-9 | 73.290 |
| Potassium chloride | 7447-40-7 | 356.000 |
| Sodium chloride | 7647-14-5 | 6699.750 |
| Sodium dihydrogen phosphate monohydrate | 10049-21-5 | 81.750 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.215 |
AMINO ACIDS
| Component | CAS Number | mg/L |
|---|---|---|
| Glycine | 56-40-6 | 24.380 |
| L-Alanine | 56-41-7 | 2.227 |
| L-Arginine hydrochloride | 1119-34-2 | 115.520 |
| L-Asparagine monohydrate | 5794-13-8 | 3.752 |
| L-Aspartic acid | 56-84-8 | 3.325 |
| L-Cysteine dihydrochloride | 8.780 | |
| L-Cystine hydrochloride monohydrate | 46.927 | |
| L-Glutamic acid | 56-86-0 | 3.675 |
| L-Glutamine | 56-85-9 | 474.500 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 36.740 |
| L-Isoleucine | 73-32-5 | 79.735 |
| L-Leucine | 61-90-5 | 82.025 |
| L-Lysine hydrochloride | 657-27-2 | 118.625 |
| L-Methionine | 63-68-3 | 23.625 |
| L-Phenylalanine | 63-91-2 | 50.740 |
| L-Proline | 147-85-3 | 8.625 |
| L-Serine | 56-45-1 | 34.125 |
| L-Threonine | 72-19-5 | 74.225 |
| L-Tryptophan | 73-22-3 | 12.510 |
| L-Tyrosine disodium salt | 79.795 | |
| L-Valine | 72-18-4 | 73.425 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 6.490 |
| D-Biotin | 58-85-5 | 0.001825 |
| D-Ca-Pantothenate | 137-08-6 | 3.120 |
| Folic acid | 59-30-3 | 3.330 |
| Nicotinamide | 98-92-0 | 3.000 |
| Pyridoxine hydrochloride | 58-56-0 | 3.015 |
| Riboflavin | 83-88-5 | 0.309 |
| Thiamine hydrochloride | 67-03-8 | 3.085 |
| Vitamin B12 | 68-19-9 | 0.340 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 3825.500 |
| DL-Thioctic acid | 1077-28-7 | 0.053 |
| HEPES buffer | 7365-45-9 | 3574.500 |
| Hypoxanthine | 68-94-0 | 1.200 |
| Linoleic acid | 60-33-3 | 0.021 |
| myo-Inositol | 87-89-8 | 9.900 |
| Phenol red sodium salt | 34487-61-1 | 12.235 |
| Putrescine hydrochloride | 333-93-7 | 0.040 |
| Sodium pyruvate | 113-24-6 | 27.525 |
| Thymidine | 50-89-5 | 0.183 |
Manufacturing & compliance
Every batch is subjected to multi-parameter release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, low total organic carbon. Supports trace-metal and organic-carbon control for sensitive culture systems.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains in the final container.
Quadruple-Stage Filtration & Per-Batch QC
Four-stage sequential filtration to a 0.04 µm final cut-off, with per-batch release testing and Certificate of Analysis.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL, tested per manufacturing batch.
Sterility — USP <71>
14-day sterility test; no growth after incubation.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 260 - 300 mOsm/kg.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMF12H-BT1X compares
Key differences in grade, filtration, and QC depth relative to conventional 0.22 µm filtered media.
| Parameter | DCP-DMF12H-BT1X (FluxMPS™) | Standard DMEM/F-12 (0.22 µm) | Competitor DMEM/F-12 (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | N/A | N/A |
| Formulation | [+] L-Glutamine, Phenol Red, 15mM HEPES, Calcium, Magnesium, 3.8255 g/L Glucose, Sodium Pyruvate / [-] Sodium Bicarbonate | Standard, bicarbonate-buffered | Standard, bicarbonate-buffered |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMF12H-BT1X.
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
