FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/F-12 (3:1), HEPES-buffered and bicarbonate-free, engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

• HEPES-buffered (15 mM), bicarbonate-free formulation for CO₂-independent or reduced-CO₂ culture
• Quadruple-stage filtration to a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2)
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
• Glucose 3.8255 g/L and sodium pyruvate 27.525 mg/L included; L-glutamine and sodium bicarbonate not added
• Manufactured under an ISO 13485:2016 quality management system; final QC and customization at Diagnocine, Totowa, NJ
• 4× BME amino acid & vitamin concentrations for robust microfluidic perfusion
• Custom pH, glucose, HEPES, salts & nutrients — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and complicating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — two dedicated prefilter + final-filter pairs run in series, capturing particulates and organisms that pass 0.22 µm filtration.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm cartridge and preserves microchannel chip geometries.

2. 2

   0.04 µm Final filtration I

   Retains fine particulates and organisms in the mycoplasma size range (typical diameter 0.2–0.3 µm) — entirely absent from standard 0.22 µm media.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge for redundant, validated bioburden reduction.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ DMEM/F-12 (3:1), 15mM HEPES, w/o L-Glutamine and Sodium Bicarbonate is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

For fully automated, long-duration perfusion systems and liquid-handling robotics, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, built on a six-stage cascade beyond the standard quadruple-stage train.

• Total particulate exclusion beyond the 0.04 µm Microfluidics Suitable tier
• Valve & sensor protection in automated dispensing systems
• Extended perfusion stability for multi-week unattended runs

Inquiry Required: the 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request this grade.

Full technical specification

Every lot of FluxMPS™ DMEM/F-12 (3:1), 15mM HEPES, w/o L-Glutamine and Sodium Bicarbonate is released against comprehensive multi-parameter QC specifications. Available pack sizes: 500 mL, 1000 mL.

Full composition (mg/L)

Every ingredient below reflects this 1X liquid formulation. Total: 52 components across 4 categories. Values are per-lot verified and reported on the Certificate of Analysis (CoA). L-Glutamine and Sodium Bicarbonate are listed as "Not added" — these components are intentionally excluded per the product formulation.

Manufacturing & compliance

Every batch is subject to multi-parameter lot-release testing before distribution.

How DCP-DMF12H-QBT1X compares

Comparison of filtration, mycoplasma barrier, water quality, and QC depth versus conventional DMEM/F-12 media for microfluidic and MPS applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DMEM/F-12 (3:1), 15mM HEPES, w/o L-Glutamine and Sodium Bicarbonate (DCP-DMF12H-QBT1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x