FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Calcium Chloride: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Calcium Chloride: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/F-12 (3:1) medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Approximately 5× fewer particles ≥10 µm (USP <788> Method 1) versus conventional 0.22 µm-filtered media.

• Quadruple-stage, four-pass sterile filtration train (0.1 µm ×2 + 0.04 µm ×2), each 0.04 µm final filter protected by its own dedicated 0.1 µm prefilter
• Endotoxin release specification: < 0.05 EU/mL, tested per manufacturing batch by LAL assay (USP <85> BET)
• DMEM/F-12 (3:1) base combining 15 mM HEPES with sodium bicarbonate (≈36.6 mM) for stable pH across CO₂-controlled and reduced-CO₂ handling
• L-Glutamine and calcium chloride excluded — defined for glutamine-supplementation and calcium-sensitive culture protocols
• 4× BME amino acid & vitamin concentrations for robust long-duration microfluidic perfusion
• Manufactured under an ISO 13485:2016 quality management system; per-lot Certificate of Analysis available
• Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for consistent trace-metal and organic-carbon control
• Custom pH, glucose, HEPES, salts & nutrients available on request — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — two dedicated prefilter + final-filter pairs run in series, capturing what a single 0.22 µm filter misses entirely.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates, protecting the first 0.04 µm cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ DCP-DMF12H-QCT1X is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) MPS Grade six-stage ultra nano-filtered variant of this formulation is available for automated bioreactor and robotic liquid-handling systems where valve and sensor longevity is critical.

• Total particulate exclusion for closed-loop perfusion systems
• Valve & sensor protection in automated dispensing hardware
• Extended perfusion stability for long-duration unattended runs

Inquiry Required: the 0.01 µm MPS Grade variant is available on request — contact support@diagnocine.com.

Full technical specification

Every lot of FluxMPS™ DCP-DMF12H-QCT1X is released against comprehensive multi-parameter QC specifications. Available sizes: 500 mL, 1000 mL.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 50 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch subjected to multi-parameter lot-release testing before distribution.

How DCP-DMF12H-QCT1X compares

Critical advantages in filtration grade, mycoplasma barrier, water quality, and QC depth versus conventional 0.22 µm filtered media.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-DMF12H-QCT1X.

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x