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FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES: 1X Liquid

Product#: DCP-DMF12H-T1X
$52.80
DCP-DMF12H-T1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains 15mM HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/F-12 (3:1) medium with 15 mM HEPES, engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm).

  • Complete DMEM/F-12 (3:1) formulation with 3.8255 g/L glucose, 474.5 mg/L L-glutamine, and 27.525 mg/L sodium pyruvate — ready to use without supplementation
  • 15 mM HEPES plus 3070 mg/L sodium bicarbonate provide dual buffering for bench-top handling and incubator culture
  • Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) — four validated passes reaching a 0.04 µm final cut-off
  • Endotoxin release specification: less than 0.05 EU/mL (LAL assay, USP <85> BET), controlled per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
  • Phenol red pH indicator included for visual monitoring of culture health
  • Custom pH, glucose, HEPES, salts & nutrient adjustments available — support@diagnocine.com
DCP-DMF12H-T1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES: 1X Liquid
  • Glucose3825.5 mg/L ([+])
  • L-Glutamine474.5 mg/L ([+])
  • Sodium Pyruvate27.525 mg/L ([+])
  • HEPES15 mM (3574.5 mg/L) ([+])
  • Osmolality (USP <785>)320 - 360 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2–8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels and complicating long-duration perfusion. FluxMPS™ targets that gap with a validated four-stage filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filter across two dedicated prefilter/final-filter pairs, supporting unobstructed flow in narrow microfluidic channels.

target

Total metabolic control

Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for tight trace-metal and organic-carbon control across every batch.

visibility

Low background for imaging

Reduced particulate baseline versus 0.22 µm filtered media supports confocal live-cell imaging and automated high-content analysis on chip. This formulation contains phenol red and riboflavin, both of which contribute to background fluorescence and absorbance — plan imaging controls accordingly.

science

Rich, stable nutrient profile

Full amino acid and vitamin complement with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm, built from two dedicated prefilter/final-filter pairs run in series.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow environment.

Performance vs. conventional media

By USP <788> Method 1 (light obscuration), FluxMPS™ carries an approximately 5× lower particle count ≥10 µm versus standard 0.22 µm filtered media (comparative estimate).

4
Validated filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final filter cut-off
Sterility & Mycoplasma: 14-day USP <71> sterility tested per lot. Mycoplasma control is by 0.1 µm and 0.04 µm mycoplasma-retentive filtration (not individually tested per lot to USP <63>). Known mycoplasma diameters fall in the 0.2–0.3 µm range, above the 0.04 µm cut-off.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DMEM/F-12 (3:1) with 15mM HEPES 1X Liquid (DCP-DMF12H-T1X) Quadruple-stage filtration system (0.1 micron x2 + 0.04 micron x2) for organ-on-a-chip and microfluidic cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → ISO Class 5 aseptic fill.
© Diagnocine® — DCP-DMF12H-T1X
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ DMEM/F-12 (3:1) with 15mM HEPES: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated perfusion bioreactors and robotic liquid-handling platforms, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation — a separate six-stage process beyond the Microfluidics Suitable line described here.

  • Total Particulate Exclusion down to 10 nm
  • Valve & Sensor Protection for automated fluidics
  • Extended Perfusion Stability for multi-week runs

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request a quote.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low particulate, filtration-controlled media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined glucose and pyruvate levels support Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-reduced perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Reduced particulate baseline for confocal microscopy and biosensor use; note phenol red and riboflavin contribute background signal — plan controls accordingly.

ConfocalBiosensorsTEER
Technical Specifications

Full technical specification

Every lot of FluxMPS™ DMEM/F-12 (3:1) with 15mM HEPES: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, Sodium Bicarbonate, Phenol Red, 15mM HEPES, Calcium, Magnesium, Glucose, Sodium Pyruvate
Appearance Clear solution (phenol red indicator present)
Osmolality USP <785> 320 - 360 mOsm/kg
Glucose 3825.5 mg/L
L-Glutamine 474.5 mg/L
Sodium Pyruvate 27.525 mg/L
HEPES 15 mM (3574.5 mg/L)
Phenol Red 12.235 mg/L
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> < 0.05 EU/mL (batch release)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, protect from light
Freeze–thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2–8°C)
CO₂ requirement ~8% CO₂, bicarbonate-derived (15 mM HEPES-supplemented for bench-top handling)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Research grade with CoA
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory 21 CFR Part 820 (QMSR) aligned
Production Micro-batch; Totowa, NJ, USA
Intended use RUO only
Formulation

Full Composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 52 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

INORGANIC SALTS

Component CAS Number mg/L
Calcium chloride dihydrate 10035-04-8 209.775
Copper sulfate pentahydrate 7758-99-8 0.000625
Disodium hydrogen phosphate anhydrous 7558-79-4 35.510
Ferric nitrate nonahydrate 7782-61-8 0.075
Ferrous sulfate heptahydrate 7782-63-0 0.209
Magnesium chloride hexahydrate 7791-18-6 14.412
Magnesium sulfate anhydrous 7487-88-9 73.290
Potassium chloride 7447-40-7 356.000
Sodium bicarbonate 144-55-8 3070.000
Sodium chloride 7647-14-5 6699.750
Sodium dihydrogen phosphate monohydrate 10049-21-5 81.750
Zinc sulfate heptahydrate 7446-20-0 0.215

AMINO ACIDS

Component CAS Number mg/L
Glycine 56-40-6 24.380
L-Alanine 56-41-7 2.227
L-Arginine hydrochloride 1119-34-2 115.520
L-Asparagine monohydrate 5794-13-8 3.752
L-Aspartic acid 56-84-8 3.325
L-Cysteine dihydrochloride   8.780
L-Cystine hydrochloride monohydrate   46.927
L-Glutamic acid 56-86-0 3.675
L-Glutamine 56-85-9 474.500
L-Histidine hydrochloride monohydrate 5934-29-2 36.740
L-Isoleucine 73-32-5 79.735
L-Leucine 61-90-5 82.025
L-Lysine hydrochloride 657-27-2 118.625
L-Methionine 63-68-3 23.625
L-Phenylalanine 63-91-2 50.740
L-Proline 147-85-3 8.625
L-Serine 56-45-1 34.125
L-Threonine 72-19-5 74.225
L-Tryptophan 73-22-3 12.510
L-Tyrosine disodium salt   79.795
L-Valine 72-18-4 73.425
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 6.490
D-Biotin 58-85-5 0.001825
D-Ca-Pantothenate 137-08-6 3.120
Folic acid 59-30-3 3.330
Nicotinamide 98-92-0 3.000
Pyridoxine hydrochloride 58-56-0 3.015
Riboflavin 83-88-5 0.309
Thiamine hydrochloride 67-03-8 3.085
Vitamin B12 68-19-9 0.340
OTHERS
myo-Inositol 87-89-8 9.900
D-Glucose 50-99-7 3825.500
DL-Thioctic acid 1200-22-2 0.053
HEPES buffer 7365-45-9 3574.500
Hypoxanthine 68-94-0 1.200
Linoleic acid 60-33-3 0.021
Phenol red sodium salt 34487-61-1 12.235
Putrescine hydrochloride 333-93-7 0.040
Sodium pyruvate 113-24-6 27.525
Thymidine 50-89-5 0.183
Custom formulations: pH, glucose, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every batch subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity with tight trace-metal and total organic carbon (TOC) control. Endotoxin-tested per USP <85>.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains in the final container.

assignment

Micro-Batch Precision

Small-batch production with tight lot-to-lot consistency in osmolality and endotoxin release — critical for reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL assay. Release specification: less than 0.05 EU/mL, controlled per manufacturing batch. Assay sensitivity 0.005 EU/mL.

Sterility — USP <71>

14-day sterility test performed per lot; no growth observed prior to release.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 320 - 360 mOsm/kg.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • Osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMF12H-T1X compares

Filtration, mycoplasma control, water quality, and QC depth compared against conventional 0.22 µm filtered media.

Parameter DCP-DMF12H-T1X (FluxMPS™) Standard DMEM/F-12 (0.22 µm) Competitor DMEM/F-12 (0.22 µm)
Grade Microfluidics Suitable Standard Standard
Formulation [+] L-Glutamine, Sodium Bicarbonate, Phenol Red, 15mM HEPES, Calcium, Magnesium, Glucose, Sodium Pyruvate Standard Standard
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate compliance check_circle Validated per lot cancel cancel
Water quality Ultrapure Type 1, 18.2 MΩ·cm Unspecified Unspecified
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle On request cancel cancel

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DMEM/F-12 (3:1) with 15mM HEPES: 1X Liquid (DCP-DMF12H-T1X).

Yes. DCP-DMF12H-T1X is Microfluidics Suitable, engineered for organ-on-a-chip, microfluidic, and MPS applications. Its 0.04 µm final filtration reduces sub-micron particulates that can accumulate in narrow microchannels.
Four sequential stages (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reduce particulates, including organisms in the 0.2–0.3 µm mycoplasma size range, well beyond what a single-pass 0.22 µm filter retains, giving an approximately 5× lower particle count ≥10 µm by USP <788> Method 1.
DCP-DMF12H-T1X is a complete DMEM/F-12 (3:1) base already containing glucose, L-glutamine, sodium pyruvate, sodium bicarbonate, and 15 mM HEPES — no base-medium supplementation is required. Serum, growth factors, or cell-type-specific additives can be added per your protocol. Contact support@diagnocine.com for custom pH, nutrient, or buffer adjustments.
This formulation contains 3070 mg/L (approximately 36.5 mM) sodium bicarbonate. By Henderson–Hasselbalch calculation, maintaining physiological pH requires approximately 8% CO₂ in the incubator atmosphere. The included 15 mM HEPES provides supplemental buffering during bench-top handling outside the incubator but does not eliminate the CO₂ requirement.
Yes. DCP-DMF12H-T1X is a basal medium compatible with FBS, human serum, growth factors, and antibiotics. Pre-filter serum and protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter before combining, to protect Microfluidics Suitable purity without stripping serum components.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet the release specification of less than 0.05 EU/mL. Request the Certificate of Analysis at support@diagnocine.com.
Yes — a full CoA is available per lot and includes: lot number, expiry, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), mycoplasma-retentive filtration status, and particulate count (USP <788> Method 1). Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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