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FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine: 1X Liquid

Product#: DCP-DM12-Q1X
$49.50
DCP-DM12-Q1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without HEPES

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. Processed through a validated four-stage filtration train reaching a 0.04 µm final pore size, it is built to be microchannel-safe from day one.

  • Quadruple-stage filtration train: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II — Polish)
  • Endotoxin release specification: < 0.05 EU/mL (USP <85> BET), tested per manufacturing batch
  • DMEM/Ham's F-12 (1:1) base with Trace Elements; supplied without L-Glutamine and without HEPES for user-defined supplementation
  • Glucose 3151 mg/L (3.151 g/L) and sodium pyruvate 110 mg/L provide a defined carbon source
  • pH 7.4 (USP <791>); sodium bicarbonate-buffered (1200 mg/L NaHCO3), 5% CO₂ recommended
  • Phenol red sodium salt included as a pH indicator (8.630 mg/L)
  • Manufactured under an ISO 13485:2016 quality management system, with aseptic fill & finish at Diagnocine, Totowa, NJ
  • Custom formulations available — pH, glucose concentration, salts, HEPES, and nutrient composition on request
DCP-DM12-Q1X |Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine: 1X Liquid
Available sizes: 500 mL, 1000 mL
  • Glucose3151 mg/L (3.151 g/L)
  • L-GlutamineNot present — supplement as needed
  • Sodium Pyruvate110 mg/L
  • HEPESNot present
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and batch-to-batch endotoxin variation that can accumulate inside microchannels and disrupt biosensor readings and primary cultures. FluxMPS™ is engineered to address these failure modes directly through its filtration architecture.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles down to sub-mycoplasma size; USP <788> Method 1 particulate compliance verified per batch.

target

Total metabolic control

Defined glucose (3151 mg/L) and sodium pyruvate (110 mg/L) with no added L-Glutamine, giving researchers precise control over the carbon and nitrogen sources for metabolic flux experiments.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm) under controlled trace-metal and total organic carbon (TOC) limits, reducing extraneous inputs into the formulation.

visibility

Low background for imaging

Low particulate counts reduce optical interference from subvisible debris, supporting confocal microscopy, live-cell biosensors, and TEER measurements.

science

Rich, stable nutrient profile

Micro-batch precision locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine: 1X Liquid is processed through a four-stage filtration train — two dedicated prefilter + final-filter pairs — reaching a 0.04 µm final pore size, retaining mycoplasma-sized particulates (0.2–0.3 µm) and subvisible particles that a single 0.22 µm pass does not address.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized material (0.2–0.3 µm) that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge downstream.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in an ISO Class 5 laminar-flow workstation.

Filtration architecture vs. conventional media

Two prefilter + final-filter pairs run in series, reaching a 0.04 µm final pore size with USP <788> Method 1 (light obscuration) particulate compliance verified on every batch — compared to a single 0.22 µm pass in conventional media.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every batch undergoes 14-day USP <71> sterility testing. Mycoplasma is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine 1X Liquid (DCP-DM12-Q1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DM12-Q1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine: 1X Liquid is suited to organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation supports microchannel integrity and laminar flow in chip-based devices.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined glucose and pyruvate concentrations support precise metabolic flux analysis.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered formulation supports sensitive iPSC differentiation protocols where endotoxin variability affects outcomes.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurements.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

Chemically defined glucose and pyruvate levels support isotope tracing studies. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate load supports confocal imaging and biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine: 1X Liquid undergoes the quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Contains Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, Glucose, Sodium Pyruvate; without L-Glutamine and HEPES
Appearance Red-colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 3151 mg/L (3.151 g/L)
L-Glutamine Not present — supplement as needed
Sodium Pyruvate 110 mg/L
Phenol Red 8.630 mg/L (present)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement 5% CO₂ (sodium bicarbonate-buffered, 1200 mg/L NaHCO3)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Grade Microfluidics Suitable
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from the manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Ammonium metavanadate   0.00058
Ammonium molybdate tetrahydrate   0.00618
Calcium chloride dihydrate 10035-04-8 154.500
Copper sulfate pentahydrate   0.0013
Disodium hydrogen phosphate   71.020
Ferric nitrate nonahydrate 7782-61-8 0.050
Ferrous sulfate heptahydrate   0.417
Magnesium chloride hexahydrate   61.200
Magnesium sulfate anhydrous 7487-88-9 48.840
Nickel chloride   0.00012
Potassium chloride 7447-40-7 311.800
Sodium bicarbonate 144-55-8 1200.000
Sodium chloride 7647-14-5 6996.000
Sodium dihydrogen phosphate monohydrate   54.300
Sodium metasillicate nonhydrate   0.0142
Sodium selenite 10102-18-8 0.00519
Stannous chloride dihydrate   0.00011
Zinc sulfate heptahydrate   0.432
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 18.750
L-Alanine 56-41-7 4.450
L-Arginine hydrochloride 1119-34-2 147.500
L-Asparagine monohydrate   7.500
L-Aspartic acid 56-84-8 6.650
L-Cysteine hydrochloride monohydrate   17.560
L-Cystine dihydrochloride 30189-89-0 31.290
L-Glutamic acid 56-86-0 7.350
L-Histidine hydrochloride monohydrate 5934-29-2 31.480
L-Isoleucine 73-32-5 54.470
L-Leucine 61-90-5 59.050
L-Lysine hydrochloride 657-27-2 91.250
L-Methionine 63-68-3 17.240
L-Phenylalanine 63-91-2 35.480
L-Proline 147-85-3 17.250
L-Serine 56-45-1 26.250
L-Threonine 72-19-5 53.450
L-Tryptophan 73-22-3 9.020
L-Tyrosine disodium salt 69847-45-6 48.100
L-Valine 72-18-4 52.850
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 8.980
D-Biotin 58-85-5 0.0035
D-Ca-Pantothenate 137-08-6 2.240
Folic acid 59-30-3 2.660
Nicotinamide 98-92-0 2.020
Pyridoxal hydrochloride 65-22-5 2.000
Pyridoxine hydrochloride 58-56-0 0.031
Riboflavin 83-88-5 0.219
Thiamine hydrochloride 67-03-8 2.170
Vitamin B12 68-19-9 0.680
myo-Inositol 87-89-8 12.600
OTHERS
D-Glucose 50-99-7 3151.000
DL-Thioctic acid   0.105
Hypoxanthine sodium salt   2.400
Linoleic acid 60-33-3 0.042
Phenol red sodium salt 34487-61-1 8.630
Putrescine hydrochloride   0.081
Sodium pyruvate 113-24-6 110.000
Thymidine 50-89-5 0.365
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production batch.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water under controlled trace-metal and TOC limits.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay per batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: See CoA.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Certificate of Analysis: Request via support@diagnocine.com with your lot number.
Product Comparison

How DCP-DM12-Q1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter DCP-DM12-Q1X (FluxMPS™) Conventional DMEM/F-12 (0.22 µm) Standard DMEM/F-12 alternative
Grade Microfluidics Suitable Conventional (0.22 µm filtered) Standard (0.22 µm filtered)
Base Formulation DMEM/Ham's F-12 (1:1) with Trace Elements, w/o L-Glutamine DMEM/F-12 Standard DMEM/F-12 Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-DM12-Q1X uses a Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, delivering low particulate counts suited to OoC and MPS microchannels. It is a Microfluidics Suitable product.
Standard 0.22 µm filtration does not retain mycoplasma-sized particulates (0.2–0.3 µm) or many subvisible particles. FluxMPS™ runs a four-stage train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, verified against USP <788> Method 1 on every lot.
This formulation is supplied without L-Glutamine so researchers can add it fresh, since glutamine degrades in liquid storage, or substitute a stabilized dipeptide such as GlutaMAX. Typical working concentrations range from 2–4 mM; contact support@diagnocine.com for a custom addition.
Yes. This formulation contains sodium bicarbonate (1200 mg/L NaHCO3) and requires a 5% CO₂ atmosphere to maintain pH 7.4.
Yes. Add FBS (5–20%), growth factors, or antibiotics per standard practice immediately before use. When filtering serum-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF membrane — the 0.04 µm final filter used for the base medium is not appropriate for protein-containing additions.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay (USP <85> BET; assay sensitivity 0.005 EU/mL) before release and must meet the specification < 0.05 EU/mL. A Certificate of Analysis is available on request.
Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788>), lot number, expiry, raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  5. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  6. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  7. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  8. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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