FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

• Quadruple-stage filtration to a 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2), with 0.1 µm mycoplasma-retentive pre-filtration stages (not tested per lot)
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> Bacterial Endotoxins Test), controlled per manufacturing batch
• Formulated without glucose, sodium pyruvate, sodium bicarbonate, or phenol red — precise metabolic control for Warburg-effect and carbon-tracing studies; L-Glutamine retained at 584 mg/L
• Bicarbonate-free formulation suited to CO₂-independent culture systems; add HEPES or a bicarbonate buffer per your application
• Prepared with Ultrapure Type 1 water (18.2 MΩ·cm), controlled for trace metals and organic carbon (TOC)
• Manufactured under an ISO 13485:2016 quality management system with ISO Class 5 (Class 100) aseptic fill and finish
• 4× BME amino acid & vitamin concentrations for robust long-duration microfluidic perfusion
• Custom pH, glucose, HEPES, salts & nutrients available — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and complicating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

This medium is produced with a validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — two paired prefilter/final-filter passes, each protecting the next.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final-filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter. The 0.04 µm pore size is smaller than the 0.2–0.3 µm diameter of mycoplasma, providing mycoplasma-retentive filtration (not tested per lot).

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow environment.

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant of this formulation is available for automated bioreactor and robotic liquid-handling platforms where valve and sensor protection is critical.

• Total particulate exclusion at the 0.01 µm cut-off for automated fluidics
• Valve & sensor protection in closed-loop perfusion systems
• Extended perfusion stability for long-duration automated culture

Inquiry Required: The 0.01 µm MPS Grade variant is available on request. Contact support@diagnocine.com.

Full technical specification

Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid is released against multi-parameter QC specifications.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 29 components across 3 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

How DCP-DMEM-GPBR1X compares

Critical advantages in filtration grade, mycoplasma-retentive filtration, water quality, and QC depth versus conventional 0.22 µm filtered media.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid (DCP-DMEM-GPBR1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x