FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Dulbecco's Modified Eagle Medium (DMEM) formulated with 25 mM HEPES and without glucose, L-glutamine, or sodium pyruvate — engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS) requiring precise, user-defined metabolic control. Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Approximately 5× fewer particles ≥10 µm than conventional 0.22 µm-filtered media.

• Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — Microfluidics Suitable purity for chip-based culture
• Endotoxin release specification: < 0.05 EU/mL (USP <85> LAL BET), tested per manufacturing batch
• Formulated without glucose, L-glutamine, and sodium pyruvate for precise, user-defined metabolic control — ideal for Warburg-effect studies and ¹³C tracing^[3]
• Contains 25 mM HEPES plus 44 mM sodium bicarbonate for flexible pH buffering across incubator and bench-top handling
• 4× BME amino acid & vitamin concentrations for robust long-duration MPS perfusion
• Manufactured under an ISO 13485:2016 quality management system; ISO Class 5 aseptic fill & finish
• Custom pH, glucose, HEPES, salts & nutrients available — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

This formulation undergoes a validated four-stage sequential filtration reaching a final pore size of 0.04 µm — capturing sub-visible particulates and mycoplasma-sized organisms that 0.22 µm filtration misses entirely.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter and preserves microchannel chip geometries.

2. 2

   0.04 µm Final filtration I

   Retains fine particulates and mycoplasma-sized organisms (typical diameter 0.2–0.3 µm) — entirely absent from standard 0.22 µm media.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter protecting the second 0.04 µm final filter, providing redundant particulate reduction.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

For automated, long-duration bioreactor and robotic perfusion systems, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered variant of this formulation — the MPS Grade line, filtered beyond the standard 0.04 µm Microfluidics Suitable cut-off.

• Total Particulate Exclusion — additional 0.02 µm and 0.01 µm polishing stages
• Valve & Sensor Protection — minimizes fouling in automated microvalve and sensor components
• Extended Perfusion Stability — supports multi-week unattended perfusion runs

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request specifications and lead time.

Full technical specification

Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 31 components across 4 categories (25 mM HEPES calculated as 5957.500 mg/L). All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

How DCP-DMEM-GQP1X compares

Critical advantages in filtration, mycoplasma barrier, water quality, and QC depth that conventional media cannot match for MPS and microfluidic applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid (DCP-DMEM-GQP1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x