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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Formulated without glucose, L-glutamine, or sodium bicarbonate, with 110 mg/L sodium pyruvate retained as an alternative carbon source for defined metabolic studies.
- Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) — two dedicated prefilter/final-filter pairs for mycoplasma-size and sub-micron particulate reduction
- Endotoxin release specification: less than 0.05 EU/mL (USP <85> BET, tested per manufacturing batch)
- Formulated without glucose, L-glutamine and sodium bicarbonate; contains 110 mg/L sodium pyruvate as an alternative carbon source
- Ultrapure Type 1 water (18.2 MΩ·cm) with tight trace-metal and organic-carbon control
- Manufactured under an ISO 13485:2016 quality management system with ISO Class 5 (Class 100) aseptic fill & finish
- 4× Basal Medium Eagle (BME) amino acid and vitamin concentrations, characteristic of the DMEM formulation
- Custom pH, glucose, HEPES, salts & nutrients available — support@diagnocine.com
- GlucoseRemoved
- L-GlutamineRemoved ([-])
- Sodium Pyruvate110 mg/L ([+])
- pH (USP <791>)7.4
- Osmolality (USP <785>)310 - 350 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, away from bright light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and complicating metabolic assays. FluxMPS™ closes that gap.[1]
Microchannel-safe purity
A 0.04 µm final filtration stage, applied twice within the quadruple-stage train, supports unobstructed flow in narrow microfluidic channels.[2]
Total metabolic control
A defined, glucose-free carbon source with 110 mg/L sodium pyruvate supports Warburg-effect studies, glycolysis inhibition, and 13C metabolic tracing.[3]
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under tight trace-metal and organic-carbon (TOC) control, supporting reproducible electrochemical biosensor and TEER-based assay conditions.
Low background for imaging
Ultra-low particulate baseline for confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch production — tight lot-to-lot consistency for long-duration MPS perfusion.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ applies a validated four-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a final pore size of 0.04 µm, run as two dedicated prefilter/final-filter pairs to reduce sub-micron particulates and mycoplasma-size organisms beyond the reach of single-stage 0.22 µm filtration.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
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3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Filtration architecture
FluxMPS™ runs two repeated prefilter/final-filter pairs in series rather than a single descending cascade: each 0.04 µm final filter is protected by its own dedicated 0.1 µm prefilter, giving full redundancy across the train.
© Diagnocine® — DCP-DMEM-GQB1X
Optimized for next-generation cell biology platforms
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available for automated bioreactor and liquid-handling robotics platforms where valve and sensor protection is critical.
- Total particulate exclusion beyond the 0.04 µm Microfluidics Suitable tier
- Valve and sensor protection for automated perfusion systems
- Extended perfusion stability in closed-loop bioreactor systems
Inquiry Required: The 0.01 µm MPS Grade variant is available by request. Contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultra-low particulate media filtered to a 0.04 µm final cut-off for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.[4]
Warburg Effect & Metabolic Research
Glucose-free formulation retaining sodium pyruvate as the sole carbon source, suited to Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.[5]
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-reduced perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined formulation for 13C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium; this formulation contains phenol red.
Microscopy & Optical Sensing
Ultra-low particulate medium for confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Full technical specification
Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid is released against comprehensive multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] Phenol Red, Calcium, Magnesium, Sodium Pyruvate · [-] Glucose, L-Glutamine, Sodium Bicarbonate |
| Appearance | Clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310 - 350 mOsm/kg |
| Glucose | Removed |
| L-Glutamine | Removed ([-]) |
| Sodium Pyruvate | 110 mg/L ([+]) |
| Phenol Red | 15.9 mg/L ([+]) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL (batch release specification) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm and 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, away from bright light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO2 requirement | Bicarbonate-free; suitable for CO2-independent culture. Add 10-25 mM HEPES for enhanced pH stability if required. |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 · Molecular biology and cell culture growth media · (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 30 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-45-6 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every batch is subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at DiagnoCine R&D Center; customization at DiagnoCine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity with tight trace-metal and organic-carbon (TOC) control. Formulated to support sensitive biosensor and TEER-based assay conditions.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains in the final container.
Micro-Batch Precision
Small-batch production with tight lot-to-lot consistency in osmolality, pH, and endotoxin — critical for reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Batch release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light Obscuration Particle Count Test performed per manufacturing batch; results reported on the Certificate of Analysis.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 310 - 350 mOsm/kg.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEM-GQB1X compares
Comparative filtration architecture, mycoplasma control, and QC depth relative to conventional 0.22 µm filtered DMEM for MPS and microfluidic applications.
| Parameter | DCP-DMEM-GQB1X (FluxMPS™) | Standard DMEM (0.22 µm) | Competitor DMEM (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard grade | Standard grade |
| Formulation definition | [+] Phenol Red, Calcium, Magnesium, Sodium Pyruvate · [-] Glucose, L-Glutamine, Sodium Bicarbonate | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (0.1 µm ×2 + 0.04 µm ×2) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| Sterility testing (USP <71>) | check_circle 14-day test per batch | Not specified | Not specified |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid (DCP-DMEM-GQB1X).
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
