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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEML-QB1X
$34.10
DCP-DMEML-QB1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose (Low) Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate

FluxMPS™ DCP-DMEML-QB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Dulbecco's Modified Eagle Medium, Low Glucose formulation, engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. The quadruple-stage filtration train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional single-stage filtration — supporting unobstructed microchannel flow and low-particulate optical baselines. This formulation is prepared without L-glutamine or sodium bicarbonate, giving researchers independent control of nitrogen supplementation and buffering chemistry for CO2-independent or custom-buffered culture systems.

  • Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final cut-off
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Low Glucose (1.0 g/L) base with 110 mg/L sodium pyruvate; formulated without L-glutamine (add fresh at time of use) and without sodium bicarbonate
  • Contains phenol red (15.9 mg/L) as a pH indicator — orange-colored, clear solution
  • Bicarbonate-free formulation intended for CO2-independent or user-buffered culture systems (e.g., HEPES-supplemented)
  • Manufactured under an ISO 13485:2016 quality management system with full lot traceability; final QC and packaging at Diagnocine, Totowa, NJ
  • 4× Basal Medium Eagle (BME) amino acid and vitamin concentrations; 31 total components across inorganic salts, amino acids, vitamins, and other constituents
  • Custom pH, glucose concentration, salts, and nutrient adjustments available on request
SKU: DCP-DMEML-QB1X Sizes: 500 mL, 1000 mL Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-GlutamineNot included
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (temperature-controlled)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma (0.2–0.3 µm diameter) and subvisible particulates that accumulate inside microfluidic channels, foul chip geometries, and add background noise to optical and sensor measurements. FluxMPS™ is designed specifically for these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration removes sub-micron particulates that can foul channels below 100 µm. USP <788> Method 1 (light obscuration) particulate testing supports safe perfusion across a wide range of chip geometries.

target

Total metabolic control

A defined Low Glucose (1.0 g/L) base with 110 mg/L sodium pyruvate and no added L-glutamine or sodium bicarbonate lets researchers set the carbon source, nitrogen source, and buffering chemistry independently for Warburg-effect and metabolic flux studies.

water_drop

Ultrapure-grade water

Every batch is prepared using Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696), manufactured under trace-metal and total organic carbon (TOC) control to minimize background contaminants.

visibility

Low background for imaging

Ultra-low particulate count from quadruple-stage filtration reduces particulate-driven background in confocal, widefield, and biosensor measurements on chip. This formulation contains phenol red (15.9 mg/L), which contributes its own optical absorbance; a phenol red–free variant is available on request for fluorescence-sensitive assays.

science

Rich, stable nutrient profile

4× Basal Medium Eagle (BME) amino acid and vitamin concentrations, micro-batch manufacturing, and per-lot QC support reproducible cell growth across multi-day perfusion experiments.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and nutrient composition are adjustable per your protocol. Contact support@diagnocine.com to specify your formulation.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage filtration train reaches a final 0.04 µm pore size under controlled aseptic fill conditions. Each 0.04 µm final filter is protected by its own dedicated 0.1 µm prefilter, giving two complete prefilter + final-filter pairs in series.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and process contaminants. Protects the first 0.04 µm cartridge from early fouling.

  2. 2

    0.04 µm Final filtration I

    Retains fine particulates and material in the mycoplasma size range (0.2–0.3 µm) — a step absent from standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    A second dedicated prefilter protecting the second 0.04 µm cartridge, providing redundancy across the full train.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill and finish in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Performance vs. conventional media

The quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration — addressing microchannel fouling and mycoplasma-size particulates that pass standard single-stage filtration.

0.04
µm final filtration
pore size — five times finer
than standard 0.22 µm media
4
Sequential filtration passes
(0.1 µm ×2 + 0.04 µm ×2)
Sterility & Mycoplasma: No bacterial or fungal growth observed after 14 days incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms typically range 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEML-QB1X Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system diagram showing four sequential stages: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? engineered for organ-on-a-chip and microfluidic MPS cell culture media | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system: four serial stages (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off for microphysiological systems and organ-on-a-chip applications.
© Diagnocine® — DCP-DMEML-QB1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEML-QB1X supports demanding cell culture platforms — from single-channel microfluidic chips to multi-organ body-on-a-chip systems — where media purity, metabolic precision, and buffering flexibility matter.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available on request for automated bioreactor perfusion systems, robotic liquid handlers, and long-duration closed-loop platforms where even trace particulates cause valve failure or sensor drift.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC methods
  • Valve & Sensor Protection: Reduces micro-fouling of solenoid valves, peristaltic pump tubing, and inline optical sensors
  • Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture without filter replacement in the chip circuit

Inquiry Required: The 0.01 µm MPS Grade is a separate, custom product. Contact support@diagnocine.com to request this variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-clean, 0.04 µm–filtered media supports unobstructed microchannel flow and laminar flow fidelity in complex multi-organ chip architectures.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Low glucose (1.0 g/L) base with user-defined nitrogen sources supports precise control of aerobic glycolysis for Warburg phenotype studies.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-low endotoxin (< 0.05 EU/mL release specification) and mycoplasma-retentive filtration support sensitive iPSC-differentiation protocols.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-reduced, endotoxin-controlled DMEM supports HUVEC and primary hepatocyte monolayer culture for barrier-function assays and TEER monitoring.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined low-glucose formulation with omitted glutamine provides a controlled metabolic background for 13C isotope tracing and extracellular flux measurement. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium; this formulation contains phenol red.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate count from quadruple-stage filtration reduces particulate background for confocal, widefield, and biosensor measurements on chip. Request the phenol red–free variant for fluorescence-sensitive imaging assays.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every batch is released against the full specification matrix below. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (Low), [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate
Appearance Orange-colored, clear solution (phenol red present)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H2O
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
L-Glutamine Not included
Sodium Pyruvate 110 mg/L
Phenol Red 15.900 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Bicarbonate-free formulation; CO2-independent when buffered (e.g., 10–25 mM HEPES added by user); validate per cell line and culture system
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

FluxMPS™ DMEM is a modification of Basal Medium Eagle (BME) containing 4× BME concentrations of amino acids and vitamins, plus glycine, serine, and ferric nitrate. Total ingredients: 31 across four formulation categories (Inorganic Salts, Amino Acids, Vitamins, Others), organized into three tabs below. Every ingredient listed is present in the released product; CAS numbers provided for traceability.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 15.900
Sodium pyruvate 113-24-6 110.000
Custom formulation: pH, glucose concentration, salts, HEPES, and nutrient composition are available on request. Contact support@diagnocine.com for custom co-formulation of DCP-DMEML-QB1X.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final packaging, QA, and testing are conducted at the Diagnocine R&D and Quality Testing Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

All formulations use Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696), manufactured under trace-metal and total organic carbon (TOC) control.

biotech

ISO Class 5 Fill & Finish

Final aseptic fill performed in a validated ISO Class 5 (Class 100) laminar-flow workstation, supporting container-closure integrity at the point of fill.

assignment

Micro-Batch Precision

Small-batch production with full per-lot traceability. Each batch is individually tested and released; a Certificate of Analysis is issued for every batch.

Endotoxin — USP <85> BET

Limulus Amebocyte Lysate (LAL) assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL, tested per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration particle count. Release limits: NMT 25/mL (≥10 µm) and NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target range: 230–270 mOsm/kg H2O.

Documentation & CoA

Full Certificate of Analysis with raw-material traceability, in-process records, and final-release test results available for every batch upon request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request: support@diagnocine.com.
Product Comparison

How DCP-DMEML-QB1X compares

FluxMPS™ DCP-DMEML-QB1X vs. conventional 0.22 µm–filtered DMEM formulations.

Parameter DCP-DMEML-QB1X (FluxMPS™) Conventional DMEM
(0.22 µm filtered)		 Standard Alt. DMEM
(0.22 µm filtered)
Grade Microfluidics Suitable Standard (0.22 µm filtered) Standard (0.22 µm filtered)
No L-Glutamine, no Sodium Bicarbonate — supports CO2-independent, user-buffered microfluidic culture check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration (0.1 µm) check_circle Yes cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel Not specified cancel Not specified
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed formulation cancel Fixed formulation

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEML-QB1X.

Yes. DCP-DMEML-QB1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, reducing particulate levels that can accumulate in microfluidic channels. It is engineered for microphysiological systems (MPS), OoC, tissue-on-a-chip (ToC), and lab-on-a-chip (LoC) platforms where sub-micron particle accumulation can cause flow obstruction and signal artifacts.
Conventional 0.22 µm filtration retains bacteria but allows mycoplasma (0.2–0.3 µm) and subvisible particulates to pass. FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II — reaching a 0.04 µm final cut-off, five times finer than a 0.22 µm membrane, with mycoplasma-retentive filtration built into the train.
This formulation is designed for CO2-independent platforms (open-top chips, atmospheric incubation) that buffer pH through HEPES (10–25 mM recommended) or another organic buffer rather than bicarbonate/CO2. L-glutamine is omitted for fresh addition at time of use (typically 2 mM), avoiding the spontaneous degradation that occurs during storage. This combination gives researchers independent control of buffering chemistry and nitrogen source — useful for multi-organ chip experiments with heterogeneous CO2 environments.
No, not as supplied. This formulation contains no sodium bicarbonate and is intended for CO2-independent culture when buffered with an alternative system such as HEPES (10–25 mM), added by the researcher. It is not formulated for use in a standard 5% CO2, bicarbonate-buffered incubator without modification.
Yes. This medium is a defined base formulation. Add FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or custom nutrients as required. When adding serum or protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane before use; do not use a 0.04 µm membrane for supplement filtration, as it removes serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch (not per unit) using a Limulus Amebocyte Lysate (LAL) assay per USP <85>, with an assay sensitivity of 0.005 EU/mL. Every batch must meet the release specification of < 0.05 EU/mL before release. A Certificate of Analysis stating the batch result is available on request.
Yes. A full CoA is issued for every batch and includes: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), particulate count (USP <788> Method 1), and raw-material traceability. Request via support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting ultra-filtered, microfluidics suitable media in organ-on-a-chip, microfluidic, and metabolic research applications.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  7. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  8. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  9. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  10. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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