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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEMH-QPB1X
$44.00
DCP-DMEMH-QPB1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains High Glucose (4500 mg/L) Without L-Glutamine Without Sodium Bicarbonate Without Sodium Pyruvate

FluxMPS™ DCP-DMEMH-QPB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and CO2-independent or open-top microfluidic platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides pH buffering independent of CO2 tension. Formulation: [+] Phenol Red, [+] HEPES (25mM), [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Sodium Pyruvate.

  • High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and aerobically active cells
  • 25 mM HEPES (pKa 7.3 at 37°C) — pH buffering independent of CO2 tension
  • 0.04 µm final filtration — sub-mycoplasma-range pore size for microfluidic channels below 100 µm
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per batch
  • Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
  • Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request
CAT. NO.
DCP-DMEMH-QPB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Sodium Pyruvate
  • AppearanceOrange-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-range particles, subvisible particulates, and process residues that can accumulate in microfluidic channels. Standard DMEM also lacks HEPES buffering, causing pH instability whenever chips are handled outside incubators. FluxMPS™ addresses both concerns simultaneously.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion in sub-100 µm channel geometries.

bolt

High-energy cell support

4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.

water_drop

HEPES: CO2-stable pH

25 mM HEPES (pKa 7.3 at 37°C) supports pH stability across CO2 fluctuation — useful for open-top chips, point-of-care devices, and atmospheric incubation.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy and biosensor platforms on chip.

science

Rich, stable nutrient profile

31 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.

tune

Customization on demand

pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under aseptic fill conditions, delivering sub-mycoplasma-range purity compared with conventional 0.22 µm media.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-DMEMH-QPB1X is processed through a repeated prefilter + final-filter pair, run twice in series, with HEPES buffering for pH stability outside incubators.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final pore size — sub-mycoplasma-range polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration at the prefiltration stages (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEMH-QPB1X Dulbecco's Modified Eagle Medium (DMEM) High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? Microfluidics Suitable DMEM High Glucose HEPES for organ-on-a-chip and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for sub-mycoplasma-range purity.
© Diagnocine® — DCP-DMEMH-QPB1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEMH-QPB1X combines high-glucose energy support with HEPES pH stability — supporting demanding platforms from open-top microfluidic chips and CO2-free bioreactors to multi-organ body-on-a-chip systems.

Automated Bioreactors & Robotics

Next-Generation System Uptime

HEPES buffering reduces CO2 dependency in automated bioreactor perfusion. An optional 0.01 µm (10 nm) MPS Grade variant is available on request for robotic liquid handlers where even trace particulates cause valve failure.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from bioreactor media lines
  • pH-Stable Automated Delivery: HEPES supports pH stability during robotic media exchanges without CO2 re-equilibration delays
  • Extended Perfusion Stability: Consistent high-glucose delivery with stable pH over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Open-Top & CO2-Free Chips

HEPES buffering enables pH-stable culture in open-top microfluidic devices, atmospheric incubators, and multi-compartment chips with heterogeneous CO2 environments.

OoCToCBoCLoCMPS
Neuroscience

Primary Neurons & Brain-on-Chip

High glucose (4.5 g/L) + HEPES stability supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion without pH drift.

iPSC-NeuronsPrimary neuronsBrain-on-chip
Cardiac Biology

Cardiomyocyte & Heart-on-Chip

High-glucose DMEM + HEPES is a standard base for iPSC-CM maturation and heart-on-chip functional assays requiring stable pH and high energy substrate.

iPSC-CMHeart-on-chipTEER
Cancer Biology

Warburg Effect & Cancer Models

High glucose supports aerobic glycolysis in cancer lines; HEPES stabilizes pH during rapid glucose consumption spikes in Warburg-active tumour models.

MCF-7MDA-MB-231HeLaA549
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation for ¹³C isotope tracing and NMR metabolomics; HEPES allows sampling outside incubators without pH artefacts. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

HEPES-stabilized pH during imaging sessions supports confocal microscopy and biosensor platforms on chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, [+] HEPES (25mM), [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Sodium Pyruvate
Appearance Orange-colored, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H2O
Total ingredients 31
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) maintains pH stability at 37°C without gas supplementation (bicarbonate-free formulation)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 31 ingredients verified per lot with CAS numbers for full raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the OTHERS group.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.900
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-DMEMH-QPB1X custom specifications — pH, glucose, HEPES concentration, salts, or nutrient modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA and testing at DiagnoCine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity with controlled trace-metal and total organic carbon (TOC) content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.

assignment

Micro-Batch Precision

Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 230–270 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-QPB1X compares

FluxMPS™ DCP-DMEMH-QPB1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEMH-QPB1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard DMEM HG + HEPES
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm) Not specified Not specified
HEPES-only High Glucose base — no L-Glutamine, no Pyruvate, no Bicarbonate check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm mycoplasma-retentive) cancel No cancel No
HEPES buffer (25 mM) check_circle Yes cancel No check_circle Yes
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-QPB1X DMEM High Glucose + 25mM HEPES.

Yes. DCP-DMEMH-QPB1X combines Quadruple-stage 0.04 µm filtration for ultra-low particulate delivery in microfluidic channels with 25 mM HEPES buffering for CO2-stable pH. High glucose (4.5 g/L) supports energy-demanding cell types — neurons, cardiomyocytes, and cancer lines — in long-duration chip perfusion. The HEPES component is particularly valuable for open-top chips where CO2 concentration fluctuates.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — Polish — with 0.1 µm mycoplasma-retentive filtration at both prefiltration stages, resulting in a substantially lower particulate profile than single-pass 0.22 µm filtration.
HEPES alone buffers pH without CO2; L-glutamine and pyruvate are left for independent fresh supplementation at user-defined concentrations and timing. This gives maximum metabolic and buffer flexibility in a HEPES-only, CO2-independent, high-glucose base — ideal for complex metabolic flux studies, multi-omics experiments, and multi-organ chips with heterogeneous incubation environments.
No. This formulation is CO2-independent — HEPES (25 mM) alone supports pH stability at 37°C without gas supplementation, since sodium bicarbonate is not present. HEPES (pKa 7.3 at 37°C) is well suited to open-top chips, point-of-care platforms, and atmospheric incubation where CO2 control is impractical.
Yes. Add FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or custom nutrients as required. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before adding, to avoid reintroducing bioburden; defined, protein-free additions may be filtered at 0.1 µm. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) before release and must meet the release specification of < 0.05 EU/mL. A Certificate of Analysis is available on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration statement, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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