FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

FluxMPS™ DCP-DMEM-QPB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose (4.5 g/L) formulation supplied without L-Glutamine, Sodium Pyruvate, or Sodium Bicarbonate — giving researchers independent control over nitrogen source, secondary carbon source, and buffering chemistry. Engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and other energy-demanding cell culture models. Formulation: [+] Phenol Red, Calcium, Magnesium, Glucose (4500 mg/L, High) | [-] L-Glutamine, Sodium Bicarbonate, Sodium Pyruvate.

• High Glucose (4500 mg/L, 4.5 g/L) formulation supporting energy-demanding cell types — primary neurons, cardiomyocytes, and cancer cell lines
• Supplied without L-Glutamine, Sodium Pyruvate, and Sodium Bicarbonate — researchers define nitrogen source, secondary carbon source, and buffering chemistry independently
• Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off — Microfluidics Suitable for sub-100 µm chip channels
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
• Contains phenol red (15.9 mg/L) as a pH indicator — phenol-red-free variants available on request
• Manufactured under an ISO 13485:2016 quality management system with full batch traceability
• Ultrapure Type 1 water (18.2 MΩ·cm) — low trace-metal and organic-carbon content
• Custom pH, glucose concentration, salts, and nutrient adjustments available — contact support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that clog microfluidic channels and corrupt sensor signals. FluxMPS™ High Glucose reduces these failure modes while delivering 4.5 g/L glucose for energy-demanding cell types and complex tissue models.

Quadruple-stage filtration system

Four serial filtration passes reaching a final 0.04 µm polish, run as two dedicated prefilter + final-filter pairs for full redundancy.

1. 1

   0.1 µm Prefiltration I

   Removes large aggregates and particulate contaminants; protects the first 0.04 µm cartridge from early fouling.

2. 2

   0.04 µm Final filtration I

   Retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill under ISO Class 5 (Class 100) conditions.

Designed for next-generation cell models

FluxMPS™ DCP-DMEM-QPB1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. High glucose (4.5 g/L) makes it particularly suited to energy-intensive cell types and aerobically active cultures.

Automated Bioreactors & Robotics

Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and optical sensors
• Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every batch released against the full specification matrix. CoA: support@diagnocine.com.

Available pack sizes: 500 mL, 1000 mL.

Full composition (mg/L)

DMEM High Glucose is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations, plus glycine, serine, and ferric nitrate. 30 ingredients verified per batch with CAS numbers for full raw-material traceability.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-DMEM-QPB1X compares

FluxMPS™ DCP-DMEM-QPB1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEM-QPB1X DMEM High Glucose.

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j