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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEM-PB1X
$34.10
DCP-DMEM-PB1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains High Glucose Without Sodium Bicarbonate Without Sodium Pyruvate

FluxMPS™ DCP-DMEM-PB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) High Glucose (4.5 g/L) DMEM formulated without sodium pyruvate and sodium bicarbonate, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and energy-demanding cell models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate.

  • High Glucose (4.5 g/L) — supports energy-demanding cell types: primary neurons, cardiomyocytes, cancer cell lines
  • Quadruple-stage filtration train — 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final pore size
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine (584 mg/L), [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate
  • Ultrapure Type 1 water (18.2 MΩ·cm) production input; manufactured under an ISO 13485:2016 quality management system
  • CO₂-independent formulation — no sodium bicarbonate present; supplement with HEPES or another organic buffer for pH control
  • Custom pH, salts, glucose concentration, and nutrient adjustments available on request
CAT. NO.
DCP-DMEM-PB1X | Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • Formulation[+] High Glucose, [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • AppearanceOrange-red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)250 – 290 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Sterility (USP <71>)No growth / 14 days
  • FiltrationQuadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-scale organisms and subvisible particulates that accumulate in microfluidic channels and interfere with optical and electrical sensing. FluxMPS™ High Glucose is processed through a finer, repeated filtration train while delivering 4.5 g/L glucose for energy-demanding cell types and complex tissue models.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion across chip geometries, including sub-100 µm channels.

bolt

High-energy cell support

4.5 g/L glucose (4× vs low-glucose DMEM) sustains primary neurons, cardiomyocytes, cancer lines, and other aerobically active cells in prolonged perfusion.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) supports a low trace-metal and organic-carbon (TOC) background relative to standard-grade purified water sources.

visibility

Low background for imaging

Ultra-low particulate baseline from 0.04 µm final filtration reduces particulate interference for confocal microscopy and optical biosensor platforms.

science

Rich, stable nutrient profile

31 ingredients verified per lot; 4× BME amino acid/vitamin concentration; micro-batch production with full traceability.

tune

Customization on demand

pH, glucose, salts, HEPES, and nutrients adjustable. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four sequential filtration stages reach a final 0.04 µm polish — two dedicated prefilter-plus-final-filter pairs in series, well beyond the single 0.22 µm pass typical of conventional media.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge from early fouling.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to fill & finish under validated ISO Class 5 conditions.

Performance vs. conventional media

5×
Lower particulate counts vs. 0.22 µm media
0.04
µm final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive membrane filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEM-PB1X Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? Microfluidics Suitable DMEM High Glucose for organ-on-a-chip and microphysiological systems | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size.
© Diagnocine® — DCP-DMEM-PB1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEM-PB1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. High glucose (4.5 g/L) makes it particularly suited to energy-intensive and aerobically active cell types.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor perfusion and robotic liquid handlers — see the Grade note above for how this differs from the standard Microfluidics Suitable product.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk to solenoid valves and optical sensors
  • Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Microphysiological Systems (MPS) & Chip

0.04 µm–filtered media reduces the risk of microchannel clogging in complex multi-organ chip architectures.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Cancer Models

High glucose (4.5 g/L) supports aerobic glycolysis phenotyping in cancer cell lines requiring elevated energy substrates.

MCF-7MDA-MB-231HeLaA549
Neuroscience

Primary Neurons & Brain-on-Chip

High glucose supports the metabolic demands of primary neurons and iPSC-derived neuronal networks in microfluidic perfusion.

iPSC-NeuronsPrimary neuronsBrain-on-chip
Cardiac Biology

Cardiomyocyte & Heart-on-Chip

High-glucose DMEM is standard for iPSC-derived cardiomyocyte maturation and heart-on-chip functional assays.

iPSC-CMHeart-on-chipTEER
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation supports 13C isotope tracing and NMR-based metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline from 0.04 µm final filtration supports confocal microscopy, biosensor integration, and TEER measurement platforms.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] High Glucose (4500 mg/L), [+] L-Glutamine (584 mg/L), [+] Phenol Red (15.9 mg/L), [+] Calcium (265 mg/L), [+] Magnesium (97.72 mg/L) | [-] Sodium Pyruvate, [-] Sodium Bicarbonate
Appearance Orange-red colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> 250 – 290 mOsm/kg H2O
Glucose 4500 mg/L (4.5 g/L, High Glucose)
L-Glutamine 584 mg/L
Sodium Pyruvate Not added (excluded from formulation)
Phenol Red 15.9 mg/L (phenol red sodium salt)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent; contains no sodium bicarbonate — use HEPES or another organic buffer for pH control
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Available pack sizes 500 mL, 1000 mL
Formulation

Full composition (mg/L)

DMEM High Glucose is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations, plus glycine, serine, and ferric nitrate. 31 ingredients verified per lot with CAS numbers for raw-material traceability.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.900
Custom formulation: Contact support@diagnocine.com for DCP-DMEM-PB1X custom specifications — pH, glucose, salts, HEPES, or nutrient modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC and testing at the Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity water input supports low trace-metal and organic-carbon (TOC) background for consistent cell culture performance.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.

assignment

Micro-Batch Precision

Small-batch production with full per-lot traceability and a Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL, controlled per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 250–290 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available for any DCP-DMEM-PB1X lot at support@diagnocine.com.
Product Comparison

How DCP-DMEM-PB1X compares

FluxMPS™ DCP-DMEM-PB1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEM-PB1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard Alt. DMEM HG
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm final) Standard grade (0.22 µm filtered) Standard grade (0.22 µm filtered)
High Glucose DMEM without Sodium Pyruvate and Sodium Bicarbonate — CO2-independent with researcher-defined carbon check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm, not tested per lot) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes (Microfluidics Suitable, 0.04 µm) cancel Higher clogging risk cancel Higher clogging risk
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEM-PB1X DMEM High Glucose.

Yes. DCP-DMEM-PB1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels suited to microphysiological systems (MPS), organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and lab-on-a-chip (LoC) platforms. High glucose (4500 mg/L) supports energy-demanding cell types including primary neurons, cardiomyocytes, and cancer cell lines in long-duration chip perfusion.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish) — reaching a 0.04 µm final pore size, well beyond the single 0.22 µm pass of conventional media. This finer, repeated filtration train is designed to reduce particulate load and the passage of mycoplasma-scale organisms (0.2–0.3 µm); mycoplasma status is not tested on a per-lot basis.
Sodium bicarbonate is excluded so researchers can implement CO2-independent buffering (for example, adding HEPES) suited to their specific chip or incubation environment. Sodium pyruvate is excluded to allow researcher-controlled titration or substitution based on the assay. Neither component is present elsewhere in this formulation, so supplementation will not risk exceeding an existing concentration.
No standard CO2 incubation is required for pH control, since this formulation contains no sodium bicarbonate. Use HEPES (10–25 mM) or another organic buffer suited to your incubation environment to maintain pH.
Yes. Fetal bovine serum (typically 5–10%), serum-free supplements, growth factors, antibiotics, or other additives can be added. When adding serum or protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — a 0.04 µm membrane is not appropriate for serum-containing additions, as it retains immunoglobulins, lipoproteins, and other serum components. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before release and must meet the release specification of < 0.05 EU/mL. A Certificate of Analysis is available on request.
Yes. A full CoA per lot covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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