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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-DMEMH-PB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and CO₂-independent or open-top microfluidic platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO₂-independent pH buffering. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] 25mM HEPES, [+] Phenol Red | [-] Sodium Pyruvate, [-] Sodium Bicarbonate.
- High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and aerobically active cells
- 25 mM HEPES (pKa 7.3 at 37°C) — robust, bicarbonate-free pH buffering independent of CO₂ tension
- Quadruple-stage filtration: Prefiltration I (0.1 µm) → Final filtration I (0.04 µm) → Prefiltration II (0.1 µm) → Final filtration II — Polish (0.04 µm)
- Contains phenol red (15.9 mg/L) as a pH indicator — orange-colored, clear solution
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Custom pH, salts, glucose, and HEPES concentration adjustments available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
- Formulation[+] L-Glutamine, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose | [-] Sodium Bicarbonate, [-] Sodium Pyruvate
- AppearanceOrange-colored, clear solution
- pH (USP <791>)7.4 ± 0.04
- Osmolality (USP <785>)230–270 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma-scale particulates and subvisible aggregates that clog microfluidic channels. Standard DMEM also lacks HEPES buffering, causing pH instability whenever chips are handled outside incubators. FluxMPS™ addresses both failure modes simultaneously.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in sub-100 µm channel geometries.
High-energy cell support
4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.
HEPES: CO₂-stable pH
25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.2–7.4 regardless of CO₂ fluctuation — useful for open-top chips, point-of-care devices, and atmospheric incubation.
Low background for imaging
Quadruple-stage 0.04 µm filtration maintains a low particulate baseline for confocal microscopy and biosensor platforms on chip. This formulation contains phenol red (15.9 mg/L); phenol-red-sensitive fluorescence or absorbance assays should account for background signal.
Rich, stable nutrient profile
32 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.
Customization on demand
pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish, delivering sub-mycoplasma-scale purity for microfluidic channels beyond what conventional 0.22 µm media provides.
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1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates and contaminants; protects the first 0.04 µm cartridge and downstream chip geometries.
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2
0.04 µm Final filtration I
Retains mycoplasma-scale particulates (0.2–0.3 µm) and sub-micron aggregates that pass a standard 0.22 µm filter.
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3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-DMEMH-PB1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMH-PB1X combines high-glucose energy support with HEPES pH stability — supporting demanding platforms from open-top microfluidic chips and CO₂-free bioreactors to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
HEPES buffering reduces CO₂ dependency in automated bioreactor perfusion. An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for robotic liquid handlers where even trace particulates cause valve failure.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from bioreactor media lines
- pH-Stable Automated Delivery: HEPES maintains pH during robotic media exchanges without CO₂ re-equilibration delays
- Extended Perfusion Stability: Consistent high-glucose delivery with stable pH over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Open-Top & CO₂-Free Chips
HEPES buffering enables pH-stable culture in open-top microfluidic devices, atmospheric incubators, and multi-compartment chips with heterogeneous CO₂ environments.
Primary Neurons & Brain-on-Chip
High glucose (4.5 g/L) + HEPES stability supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion without pH drift.
Cardiomyocyte & Heart-on-Chip
High-glucose DMEM + HEPES is a standard base for iPSC-CM maturation and heart-on-chip functional assays requiring stable pH and high energy substrate.
Warburg Effect & Cancer Models
High glucose supports aerobic glycolysis in cancer lines; HEPES stabilizes pH during rapid glucose consumption spikes in Warburg-active tumour models.
Metabolic Flux Analysis
Defined high-glucose formulation supports ¹³C isotope tracing and NMR metabolomics; HEPES allows sampling outside incubators without pH artefacts. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.
Microscopy & Optical Sensing
HEPES-stabilized pH during imaging sessions; quadruple-stage 0.04 µm filtration maintains a low particulate baseline for confocal and biosensor platforms. Contains phenol red (15.9 mg/L) — account for background absorbance in optical assays.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate |
| Appearance | Orange-colored, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | Not added |
| Phenol Red | 15.9 mg/L (phenol red sodium salt) |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| pH USP <791> | 7.4 ± 0.04 |
| Osmolality USP <785> | 230–270 mOsm/kg H₂O |
| Total ingredients | 32 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Controlled by 0.04 µm barrier filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Available pack sizes: 500 mL, 1000 mL.
Full composition (mg/L)
DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 32 ingredients verified per lot with CAS numbers for full raw-material traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QA and testing at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity, ASTM D1193 Type I equivalent — controlled trace-metal and total organic carbon (TOC) content.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, ensuring container-closure integrity.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.
Endotoxin — USP <85> BET
LAL assay, release specification: < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 230–270 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMH-PB1X compares
FluxMPS™ DCP-DMEMH-PB1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMH-PB1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG + HEPES (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Conventional 0.22 µm (no tier) | Conventional 0.22 µm (no tier) |
| HEPES-only CO₂-independent High Glucose DMEM — no Pyruvate, L-Glutamine included | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle Yes (0.04 µm) | cancel No | cancel No |
| HEPES buffer (25 mM) | check_circle Yes | cancel No | check_circle Yes |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMH-PB1X DMEM High Glucose + 25mM HEPES.
Supporting literature
Key peer-reviewed publications supporting HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j