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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEML-B1X
$34.10
DCP-DMEML-B1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose (Low) Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-DMEML-B1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Low Glucose DMEM formulated without sodium bicarbonate, built for microphysiological systems (MPS), organ-on-a-chip (OoC), and other microfluidic tissue models. The 0.04 µm final cut-off is 5.5× finer than the 0.22 µm membranes used in conventional sterile filtration, supporting unobstructed flow in sub-100 µm channel geometries.

  • Low glucose (1.0 g/L) DMEM formulated without sodium bicarbonate — compatible with HEPES buffering or CO2-independent culture systems
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, five-and-a-half times finer than a 0.22 µm sterile filter
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Contains L-glutamine (584 mg/L), sodium pyruvate (110 mg/L), and phenol red sodium salt (15.900 mg/L) as a pH indicator
  • Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis; final QC at Diagnocine, Totowa, NJ
  • Microfluidics Suitable grade (0.04 µm final filtration) engineered for OoC, MPS, and other microfluidic tissue-chip platforms
  • Custom pH, glucose, salts, and nutrient adjustments available on request
SKU: DCP-DMEML-B1X | Size: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Bicarbonate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-Glutamine584 mg/L
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)240–280 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma (0.2–0.3 µm diameter) and subvisible particulates that accumulate inside microfluidic channels, foul chip geometries, and add background to optical and electrical measurements. FluxMPS™ is built around a filtration architecture that addresses these failure modes directly.

filter_alt

Microchannel-safe purity

0.04 µm final filtration removes sub-micron particulates that can obstruct channels below 100 µm. Particulate levels are controlled to USP <788> Method 1 (light obscuration) release limits.

target

Total metabolic control

A low-glucose (1.0 g/L) base with defined sodium pyruvate and no added bicarbonate lets researchers set the carbon source and buffering system precisely for Warburg-effect and metabolic flux protocols.

water_drop

Ultrapure-grade water

Every batch is prepared with Ultrapure Type 1 water (18.2 MΩ·cm), controlling trace metals and organic carbon (TOC) in the feed water used to formulate the medium.

visibility

Low background for imaging

Ultra-low particulate carryover reduces scatter background for confocal, widefield, and biosensor measurements on chip. This formulation contains phenol red sodium salt (15.900 mg/L), which contributes visible-range absorbance — request a phenol red–free formulation for autofluorescence-sensitive assays.

science

Rich, stable nutrient profile

4× BME amino acid & vitamin concentrations, micro-batch manufacturing, and per-lot QC support reproducible cell growth across multi-day perfusion experiments.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and nutrient composition are adjustable per protocol. Contact support@diagnocine.com to specify your formulation.

Purity Architecture

Quadruple-stage filtration system

DCP-DMEML-B1X is processed through four serial filtration passes reaching a final 0.04 µm polish: a repeated pre-filter + final-filter pair, run twice in series for full redundancy.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm final filter from early fouling. Mycoplasma-retentive grade.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge; this is redundancy in the train, not a polishing step downstream of Stage 2.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill and finish.

Filtration architecture, by the numbers

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off — five-and-a-half times finer than the 0.22 µm membranes used for conventional sterile filtration of cell culture media.

5.5×
Finer final pore size
than conventional
0.22 µm filtration
4
Sequential filtration
passes (0.1 µm ×2 +
0.04 µm ×2)
Sterility & Mycoplasma: No bacterial or fungal growth observed after 14 days of incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration at Prefiltration I and Prefiltration II (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEML-B1X Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system diagram showing four sequential stages: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, and 0.04 μm Final filtration II Polish ? Microfluidics Suitable media for organ-on-a-chip and MPS applications | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system: four serial passes (0.1 µm ×2 + 0.04 µm ×2) delivering a 0.04 µm final cut-off for microphysiological systems and organ-on-a-chip applications.
© Diagnocine® — DCP-DMEML-B1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEML-B1X supports demanding cell culture platforms — from single-channel microfluidic chips to multi-organ body-on-a-chip systems — where media purity, metabolic precision, and optical clarity matter.

Automated Bioreactors & Robotics

Next-Generation System Uptime

A separate 0.01 µm (10 nm) MPS Grade variant of this formulation is available on request for automated bioreactor perfusion systems, robotic liquid handlers, and long-duration closed-loop platforms where trace particulates cause valve failure or sensor drift.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates not addressed by 0.04 µm filtration
  • Valve & Sensor Protection: Reduces micro-fouling of solenoid valves, peristaltic pump tubing, and inline optical sensors
  • Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture without in-line filter replacement

Inquiry Required: The 0.01 µm MPS Grade variant is a custom product. Contact support@diagnocine.com to request it for your automated system.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm–filtered, bicarbonate-free media supports laminar flow fidelity and independent pH control in complex multi-organ chip architectures.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Low glucose (1.0 g/L) base with defined sodium pyruvate enables precise control of aerobic glycolysis for Warburg-phenotype studies.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Low endotoxin (< 0.05 EU/mL release specification) and quadruple-stage filtration support sensitive iPSC-differentiation protocols.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particulate-controlled, endotoxin-tested DMEM supports HUVEC and primary hepatocyte monolayer integrity for barrier-function assays and TEER monitoring.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

A defined low-glucose formulation with sodium pyruvate provides a controlled metabolic background for 13C isotope tracing and extracellular flux measurement. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium — this formulation contains phenol red sodium salt.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate carryover reduces scatter background for confocal imaging, biosensor arrays, and TEER measurements on chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot is released against the full specification matrix below. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (Low), [+] Sodium Pyruvate | [-] Sodium Bicarbonate
Appearance Orange-colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> 240–280 mOsm/kg H2O
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
L-Glutamine 584 mg/L
Sodium Pyruvate 110 mg/L
Phenol Red 15.900 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification — see §Manufacturing & Compliance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100) laminar flow
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Bicarbonate-free; not buffered for standard 5% CO2 incubation — requires user-added HEPES (10–25 mM recommended) or use in CO2-independent / open microfluidic systems
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

FluxMPS™ DMEM is a modification of Basal Medium Eagle (BME) containing 4× BME concentrations of amino acids and vitamins, plus glycine, serine, and ferric nitrate. Total: 32 components across 4 categories. Every ingredient below is present in the released product; CAS numbers provided for traceability.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 15.900
Sodium pyruvate 113-24-6 110.000
Custom formulation: pH, glucose concentration, salts, HEPES, and nutrient composition are available on request. Contact support@diagnocine.com for custom co-formulation of DCP-DMEML-B1X.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured by ISO 13485:2016–certified suppliers. Final packaging, QA, and testing are conducted at the Diagnocine R&D and Quality Testing Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

All formulations use Ultrapure Type 1 water (18.2 MΩ·cm), controlling trace metals and organic carbon in the feed water used to prepare the medium.

biotech

ISO Class 5 Fill & Finish

Final aseptic fill performed in a validated ISO Class 5 (Class 100) laminar-flow workstation, supporting container-closure integrity at the point of fill.

assignment

Micro-Batch Precision

Small-batch production with full per-lot traceability. Each batch is individually tested and released — no blending of lots. A Certificate of Analysis is issued for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL, tested per batch.

Particulate — USP <788> Method 1

Light obscuration particle count. Release limits: NMT 25/mL (≥10 µm) and NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target range: 240–280 mOsm/kg H2O.

Documentation & CoA

Full Certificate of Analysis with raw-material traceability, in-process records, and final-release test results available for every lot upon request.

Certificate of Analysis: Request the CoA for any lot of DCP-DMEML-B1X by emailing support@diagnocine.com.
Product Comparison

How DCP-DMEML-B1X compares

FluxMPS™ DCP-DMEML-B1X vs. conventional 0.22 µm–filtered DMEM formulations.

Parameter DCP-DMEML-B1X (FluxMPS™) Conventional / competitor media
Grade Microfluidics Suitable Not specified (typically 0.22 µm sterile filtration only)
No sodium bicarbonate — HEPES-compatible / CO2-independent option check_circle Yes cancel No (bicarbonate-buffered)
Final filtration pore size 0.04 µm 0.22 µm
Number of filtration stages 4 (0.1 µm ×2 + 0.04 µm ×2) 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stage) cancel Not typically included
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel Not specified
Water quality Ultrapure Type 1, 18.2 MΩ·cm Not specified
Manufacturing QMS ISO 13485:2016 Not specified
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of channel fouling
Custom formulation check_circle Available cancel Fixed formulation

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEML-B1X.

Yes. DCP-DMEML-B1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels that reduce microchannel clogging risk. It is Microfluidics Suitable and formulated without sodium bicarbonate, which is useful for open-top or HEPES-buffered OoC, ToC, and LoC platforms.
Conventional 0.22 µm filtration retains bacteria but does not target mycoplasma (0.2–0.3 µm) or subvisible particulates. FluxMPS™ runs four sequential passes — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — reaching a 0.04 µm final cut-off, 5.5× finer than a 0.22 µm membrane.
Sodium bicarbonate is omitted so that pH can be maintained with an alternative buffer such as HEPES (10–25 mM recommended) instead of a CO2-dependent bicarbonate system. This suits open-top microfluidic devices, ambient incubation, or protocols requiring independent pH control. L-glutamine and sodium pyruvate remain in the base formulation and are not affected by this change.
No, not as formulated. DCP-DMEML-B1X contains no sodium bicarbonate and is not buffered for a standard 5% CO2 incubator. Use it with a user-added buffer such as HEPES (10–25 mM) for sealed incubation, or in CO2-independent / open microfluidic systems.
Yes. This is a defined base formulation. Add FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or a buffer such as HEPES as required. When filtering serum-containing or protein-containing additions, use a 0.2 µm low-protein-binding PES or PVDF membrane — never 0.04 µm, which retains IgM, lipoproteins, and much of the functional serum fraction. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Each batch is tested by LAL assay per USP <85> (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. A Certificate of Analysis for the specific lot is available on request.
Yes. A full CoA is issued for every lot and includes: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request via support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting the use of ultra-filtered media in organ-on-a-chip, microfluidic, and metabolic research applications.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  7. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  8. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
  10. Ashammakhi N, et al. Kidney-on-a-chip: untapped opportunities. Kidney Int. 2018;94:1073–1086. doi:10.1016/j.kint.2018.06.034

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