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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM) Low Glucose w/o L-Glutamine: 1X Liquid

Product#: DCP-DMEML-Q1X
$34.10
DCP-DMEML-Q1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
Cell Culture Media — FluxMPS™ Series
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), Low Glucose w/o L-Glutamine: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose (Low, 1.0 g/L) Contains Sodium Pyruvate Without L-Glutamine Without HEPES

FluxMPS™ DCP-DMEML-Q1X is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Dulbecco’s Modified Eagle Medium formulated with 1.0 g/L glucose, sodium pyruvate, and sodium bicarbonate — without L-Glutamine — giving researchers full control of nitrogen source supplementation. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage nano-filtration: 0.1 µm × 2 passes + 0.04 µm × 2 passes, alternating prefilter and final-filter pairs for full redundancy
  • Ultra-low particulate, microchannel-safe formulation — suitable for organ-on-a-chip (OoC) and microphysiological system (MPS) research
  • Endotoxin release specification < 0.05 EU/mL (USP <85> BET, LAL assay); sterility confirmed per USP <71> after 14-day incubation
  • Formulation: [+] Low Glucose 1.0 g/L, [+] Sodium Pyruvate 110 mg/L, [+] Sodium Bicarbonate 3700 mg/L, [+] Phenol Red 15.9 mg/L; [−] L-Glutamine, [−] HEPES
  • Prepared with Ultrapure Type 1 water (18.2 MΩ·cm), supporting low trace-metal and organic-carbon (TOC) background
  • ISO Class 5 (Class 100) aseptic fill & finish; manufactured under an ISO 13485:2016 quality management system
  • Customizable — L-Glutamine, HEPES, pH, glucose level, and other nutrients available on request
DCP-DMEML-Q1X Size: 500 mL and 1000 mL Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
FluxMPS™ DMEM Low Glucose w/o L-Glutamine — 1X Liquid
  • Glucose1000 mg/L (1.0 g/L)
  • L-GlutamineNot included — add exogenously
  • Sodium Pyruvate110 mg/L
  • pH (1× conc.)7.4 (USP <791>)
  • Osmolality300–340 mOsm/kg (USP <785>)
  • Endotoxin< 0.05 EU/mL (USP <85> BET)
  • Filtration0.1µm×2 + 0.04µm×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2–8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm sterilization is inadequate for microfluidic platforms. Subvisible particulates accumulate in narrow channels, mycoplasma-sized contaminants can pass through, and elevated endotoxin levels corrupt cytokine signaling and sensor readings. FluxMPS™ DCP-DMEML-Q1X was designed for microphysiological systems, organ-on-a-chip devices, and high-precision metabolic studies.[1,2]

filter_alt

Microchannel-Safe Purity

0.04 µm final filter supports USP <788> Method 1 particulate compliance. Sub-visible particles that clog microfluidic channels are reduced at every stage of the filtration train.

target

Total Metabolic Control

L-Glutamine-free formulation gives you full control of the nitrogen source. Supplement with stable glutamine, GlutaMAX™, or custom concentrations to tune Warburg or oxidative metabolism.

water_drop

Ultrapure-Grade Water

Prepared using Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696), supporting a low trace-metal and organic-carbon (TOC) background for electrochemical biosensors and NMR metabolomics.

visibility

Low Background for Imaging

Ultra-low particulate baseline reduces scatter-related background in confocal and live-cell imaging. Note: this formulation contains phenol red (15.9 mg/L); a phenol-red-free variant is available on request for low-autofluorescence applications.

science

Rich, Stable Nutrient Profile

4× BME amino acid and vitamin concentrations, micro-batch production, and controlled fill weight support lot-to-lot consistency for longitudinal MPS and organoid studies.

tune

Customization on Demand

pH, glucose concentration, L-Glutamine, HEPES, salts, and nutrient composition available on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ DCP-DMEML-Q1X is processed through a validated four-stage filtration train reaching a 0.04 µm final pore size — two dedicated prefilter + final-filter pairs run in series inside an ISO Class 5 cleanroom, rather than a single descending cascade.

  1. 1

    0.1 µm Prefiltration I — Large Particulate Removal

    Removes large aggregates, cell debris, and bulk particulates ahead of the first 0.04 µm final filter, protecting that cartridge from premature fouling.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains fine particulates and sub-micron aggregates that a single-pass 0.22 µm filter would not remove.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated 0.1 µm prefilter protects the second 0.04 µm cartridge, providing redundancy rather than reprocessing the same effluent.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate 0.04 µm polishing pass immediately before aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

Particulate analysis under USP <788> Method 1 (light obscuration) shows FluxMPS™ media consistently delivering approximately 5× fewer subvisible particles (≥10 µm) than leading 0.22 µm-filtered alternatives. In microfluidic channels with hydraulic diameters of 50–200 µm, this difference is the boundary between unrestricted flow and progressive channel fouling.

5×
Cleaner than 0.22 µm media
by USP <788> particulate count
0.04
µm final filter pore size across
4 filtration passes
Sterility & Mycoplasma Control: Every lot undergoes 14-day sterility testing per USP <71> with no bacterial or fungal growth detected. Mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEML-Q1X Dulbecco's Modified Eagle Medium DMEM Low Glucose without L-Glutamine 1X Liquid, Quadruple-stage filtration 0.1 micron x2 and 0.04 micron x2, for organ-on-a-chip and microfluidic MPS applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-Stage Filtration System: Stage 1 (0.1 µm prefilter) → Stage 2 (0.04 µm final filter) → Stage 3 (0.1 µm prefilter) → Stage 4 (0.04 µm final filter, ISO Class 5 aseptic fill).
© Diagnocine® — DCP-DMEML-Q1X
Applications

Research applications for FluxMPS™ DMEM Low Glucose w/o L-Glutamine

The L-Glutamine-free, low glucose formulation of DCP-DMEML-Q1X is suited for metabolic flux studies, glutamine restriction experiments, Warburg-effect investigations, and any system where precise nutrient control is paramount. Its Microfluidics Suitable purity enables deployment across organ-on-a-chip, microfluidic, and automated bioprocessing platforms.[3,4]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For the most demanding automated perfusion systems, an optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of FluxMPS™ DMEM Low Glucose is available by special order. This grade provides total exclusion of nanoparticulates that could foul micro-valve actuators, optical flow sensors, and precision dosing systems in robotic cell culture platforms.

  • Total Particulate Exclusion: 10 nm filtration eliminates particulates that could trigger false-positive sensor events or coat quartz crystal microbalance sensors.
  • Valve & Sensor Protection: Reduces protein aggregate deposition on micro-solenoid valve seats and PDMS channel walls during extended perfusion runs.
  • Extended Perfusion Stability: Supports consistent ionic composition and pH over multi-week automated runs.

Inquiry Required: The 0.01 µm (10 nm) MPS Grade variant is produced on a micro-batch schedule. Contact support@diagnocine.com to request this grade and discuss lead times.

Microfluidics

Micro Physiological System (MPS) & Chip

Microfluidics Suitable purity (0.04 µm final filtration) supports unrestricted flow in narrow channels. Suitable for gut-on-a-chip, liver-on-a-chip, kidney-on-a-chip, and multi-organ body-on-a-chip devices.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

L-Glutamine-free base allows precise glutamine titration to study glutaminolysis, IDH mutations, and glutamine-driven anaplerosis. Low glucose (1 g/L) supports partial aerobic glycolysis phenotypes.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Low glucose with customizable glutamine supports metabolic reprogramming studies during iPSC differentiation into neurons, cardiomyocytes, and hepatocytes where nutrient sensitivity is critical.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Low endotoxin release specification (< 0.05 EU/mL) supports clean cytokine profiling and shear-stress experiments in primary HUVECs and HAECs.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined, L-Glutamine-free base is suited for stable-isotope tracing experiments. Add uniformly labeled 13C-glutamine at precise concentrations for flux mapping. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate background supports confocal imaging, TEER monitoring, fluorescent reporter cell lines, and integrated biosensor arrays. This formulation contains phenol red; a phenol-red-free variant is available on request.

ConfocalBiosensorsTEER
Technical Specifications

Analytical specification sheet — DCP-DMEML-Q1X

Parameters are measured per production batch and reported in the accompanying Certificate of Analysis (CoA). USP and ISO method references are provided for each critical quality attribute.

Physical & Chemical Parameters
Parameter Specification
Formulation DMEM 1X, Low Glucose; [+] D-Glucose 1.0 g/L, [+] Sodium Pyruvate 110 mg/L, [+] Sodium Bicarbonate 3700 mg/L, [+] Phenol Red 15.9 mg/L; [−] L-Glutamine, [−] HEPES ISO 13485
Appearance Red-colored, clear liquid solution (phenol red present)
pH (1×) 7.4 USP <791>
Osmolality 300–340 mOsm/kg H2O USP <785>
D-Glucose 1000 mg/L (1.0 g/L)
L-Glutamine Not present — add exogenously
Sodium Pyruvate 110 mg/L
Phenol Red 15.9 mg/L (sodium salt)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin (BET) < 0.05 EU/mL, batch release specification USP <85>
Sterility No growth after 14 days USP <71>
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm Compliant USP <788> Method 1
Particulate ≥25 µm Compliant USP <788> Method 1
Water purity Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696)
Manufacturing std. ISO 13485:2016 ISO 13485
Fill environment ISO Class 5 (Class 100) cleanroom
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack (2–8°C)
CO2 requirement 10% CO2 humidified atmosphere (NaHCO3 buffer system, 3700 mg/L)
Pack sizes 500 mL, 1000 mL
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade or better
Traceability Full lot traceability with CoA available
Manufacturing QMS ISO 13485:2016 certified ISO 13485
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Production method Micro-batch; final assembly & QC at DiagnoCine Precision, Totowa, NJ, USA
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

FluxMPS™ DCP-DMEML-Q1X contains 4× BME amino acid and vitamin concentrations. All ingredient quantities are nominal per-lot targets; actual values are reported in the CoA.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 15.900
Sodium pyruvate 113-24-6 110.000
Customization Available: L-Glutamine, HEPES, alternative glucose concentrations, additional supplements, and custom pH can be added on request. Contact support@diagnocine.com for custom formulation quotes.
Quality Assurance

Manufacturing standards & compliance

Every batch of FluxMPS™ DCP-DMEML-Q1X is manufactured under a quality system combining ISO 13485:2016 certification, USP-grade analytical testing, and ISO Class 5 aseptic fill.

verified

ISO 13485:2016 QMS

Full quality management system certified to ISO 13485:2016. All manufacturing, testing, and documentation procedures are audit-ready and traceable to source raw materials.

water_drop

Ultrapure Type 1 Water

Media are compounded using Ultrapure Type 1 water (18.2 MΩ·cm). Resistivity is monitored during production to control trace-metal and organic-carbon background.

biotech

ISO Class 5 Fill & Finish

Final filtration and container filling are performed within an ISO Class 5 (Class 100) unidirectional airflow enclosure, following the 0.04 µm final filter stage.

assignment

Micro-Batch Precision

Produced in small, controlled micro-batches at DiagnoCine Precision, Totowa, New Jersey, USA, supporting lot-to-lot consistency.

Endotoxin — USP <85> BET

Limulus Amebocyte Lysate (LAL) assay per USP <85>; assay sensitivity 0.005 EU/mL. Batch release specification: < 0.05 EU/mL. Result documented on the CoA.

Particulate Matter — USP <788> Method 1

Light obscuration particle count per USP <788> Method 1. Both ≥10 µm and ≥25 µm particle counts are reported on every CoA.

Osmolality — USP <785>

Osmolality measured by freezing-point depression per USP <785>. Release range: 300–340 mOsm/kg H2O for DCP-DMEML-Q1X.

Documentation — Certificate of Analysis (CoA)

Full CoA including lot number, test dates, analyst, and all QC results available. Request at support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com.
Product Comparison

How DCP-DMEML-Q1X compares

FluxMPS™ DCP-DMEML-Q1X was built for microfluidic and MPS applications. Where a sourced, published specification exists for a named competitor, it is shown below; all other cells read “Not specified” rather than an unattributed estimate.

Parameter DCP-DMEML-Q1X
(FluxMPS™)		 Other Published Supplier Specifications
Grade Microfluidics Suitable Not specified
Formulation feature Low glucose (1.0 g/L), no L-Gln, NaHCO3-buffered Not specified
Final filtration pore size 0.04 µm Not specified
Number of filtration stages 4 (0.1 µm ×2 + 0.04 µm ×2) Not specified
Mycoplasma-retentive filtration check_circle 0.1 µm stage Not specified
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate compliance check_circle Not specified
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Not specified
Manufacturing QMS ISO 13485:2016 Not specified
Microfluidic channel compatibility check_circle Not specified
Custom formulation check_circle Not specified

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as “Not specified”.

FAQ

Frequently asked questions

Answers to common questions about FluxMPS™ DCP-DMEML-Q1X from organ-on-a-chip researchers, metabolic biologists, and MPS platform engineers.

Yes. DCP-DMEML-Q1X was developed for organ-on-a-chip and microphysiological system (MPS) platforms. The endotoxin release specification (< 0.05 EU/mL) also reduces the risk of non-specific immune-cell activation in co-culture chip models.
Four distinct filtration stages — two 0.1 µm prefilter passes paired with two 0.04 µm final-filter passes — remove particulates that a single 0.22 µm pass cannot. The 0.04 µm final pore size approaches the size range of the smallest mycoplasma species (0.2–0.3 µm) and reduces nano-scale particulate aggregates that can accumulate in PDMS microchannels over time. Filtration occurs inside an ISO Class 5 cleanroom.
L-Glutamine is omitted intentionally to give researchers full control over nitrogen source availability. This is useful for: (1) metabolic flux studies where you want to add uniformly labeled 13C-glutamine; (2) glutamine restriction experiments studying metabolic reprogramming; (3) use of stable glutamine dipeptides such as L-Alanyl-L-Glutamine (GlutaMAX) at defined concentrations. To supplement, add L-Glutamine to a final concentration of 2–4 mM (approximately 292–584 mg/L) or your preferred dipeptide equivalent. Contact support@diagnocine.com if you need a pre-formulated version with glutamine included.
Yes. DCP-DMEML-Q1X uses a sodium bicarbonate (NaHCO3) buffer system at 3700 mg/L (approximately 44 mM). To maintain pH 7.4, this formulation requires approximately 10% CO2, calculated from the bicarbonate concentration via the Henderson-Hasselbalch equation, rather than the conventional 5% used with lower-bicarbonate formulations. If a CO2-independent system is required, contact support@diagnocine.com to request a HEPES-buffered custom formulation.
Yes. DCP-DMEML-Q1X is a basal medium compatible with standard cell culture supplements, including FBS (typically 5–10%), defined serum substitutes, GlutaMAX, antibiotics, growth factors, and extracellular matrix proteins added under standard aseptic conditions. Serum and other protein-containing additions should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane immediately before use; do not use a 0.04 µm membrane for supplement filtration, as it will retain serum proteins and lipoproteins. For serum-free or chemically defined formulations, contact support@diagnocine.com.
Endotoxin is controlled per manufacturing batch, not per unit. Each batch is tested using a Limulus Amebocyte Lysate (LAL) assay per USP <85> Bacterial Endotoxins Test, with an assay sensitivity of 0.005 EU/mL, against a release specification of < 0.05 EU/mL. The result for the applicable lot is reported on the Certificate of Analysis. This specification is relevant for MPS co-culture models incorporating immune cells, endothelial cells, or NF-kB reporter systems.
Yes. A Certificate of Analysis is available for every production batch of DCP-DMEML-Q1X. The CoA includes: lot number and manufacture date, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin result (USP <85> BET), sterility result (USP <71>), particulate matter data (USP <788> Method 1), cultural response (cell morphology and count vs. control), and analyst signature. Request your CoA at support@diagnocine.com with your lot number.
Scientific References

Supporting literature

The following peer-reviewed references support the science underlying FluxMPS™ Microfluidics Suitable media design, organ-on-a-chip methodology, and low-glucose metabolic research applications relevant to DCP-DMEML-Q1X.

  1. Bhatia, S.N. & Ingber, D.E. Microfluidic organs-on-chips. Nature Biotechnology 32, 760–772 (2014).doi:10.1038/nbt.2989
  2. Huh, D. et al. Reconstituting organ-level lung functions on a chip. Science 328, 1662–1668 (2010).doi:10.1126/science.1188302
  3. Warburg, O. On the origin of cancer cells. Science 123, 309–314 (1956).doi:10.1126/science.123.3191.309
  4. DeBerardinis, R.J. et al. The biology of cancer: Metabolic reprogramming fuels cell growth and proliferation. Cell Metabolism 7, 11–20 (2008).doi:10.1016/j.cmet.2007.10.002
  5. Bhimji, S.S. et al. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology 22, 1–27 (1996).doi:10.1007/BF00353994
  6. van der Meer, A.D. & van den Berg, A. Organs-on-chips: breaking the in vitro impasse. Integrative Biology 4, 461–470 (2012).doi:10.1039/c2ib00176d
  7. Ingber, D.E. Human organs-on-chips for disease modelling, drug development and personalized medicine. Nature Reviews Genetics 23, 467–491 (2022).doi:10.1038/s41576-022-00466-9
  8. Metallo, C.M. et al. Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia. Nature 481, 380–384 (2012).doi:10.1038/nature10602
  9. Huang, H. et al. Role of glutamine and interlinked asparagine metabolism in vessel formation. EMBO Journal 36, 2334–2352 (2017).doi:10.15252/embj.201695518
  10. Jang, K.J. et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integrative Biology 5, 1119–1129 (2013).doi:10.1039/c3ib40049b

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