FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/o Sodium Pyruvate, Calcium Chloride & D-Calcium Pantothenate: 1X Liquid

verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/o Sodium Pyruvate, Calcium Chloride & D-Calcium Pantothenate: 1X Liquid

FluxMPS™ DCP-DMEMWOSPCCCP1X is an MPS-grade, ultra-filtered DMEM High Glucose — Calcium-Free & Pantothenate-Free formulation engineered for specialized research applications where precise formulation control is essential. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate | [-] Sodium Pyruvate, [-] Calcium Chloride, [-] D-Calcium Pantothenate.

Calcium chloride–free — for trypsin dissociation, calcium-signaling, and tight-junction permeability assays
D-Calcium pantothenate–free — for CoA biosynthesis, pantothenate kinase (PanK) research, and lipid metabolism studies
Sodium pyruvate–free — add at defined concentration for precise secondary carbon control
0.04 µm final nano-filtration — sub-mycoplasma purity for microfluidic channels below 100 µm
Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
Endotoxin < 0.05 EU/mL (LAL, USP <85>)
Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
Custom formulation modifications available on request

CAT. NO.

DCP-DMEMWOSPCCCP1X | UNSPSC 41122100 Cell Culture Media

Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/o Sodium Pyruvate, Calcium Chloride & D-Calcium Pantothenate: 1X Liquid

Glucose4500 mg/L (4.5 g/L, High Glucose)
Key featureCalcium-Free & Pantothenate-Free
Formulation[+] High Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [-] Sodium Pyruvate, [-] Calcium Chloride, [-] D-Calcium Pantothenate
AppearanceRed-colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)330.00–370.00 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2–8°C, away from light
Shelf Life12 months

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered for calcium-sensitive assays and CoA pathway research

Removing calcium chloride and D-calcium pantothenate simultaneously addresses two distinct research needs: (1) calcium-free conditions for trypsin-based cell dissociation, calcium-signaling studies, and tight-junction permeability assays where extracellular calcium must be precisely controlled; (2) pantothenate-free conditions for coenzyme A (CoA) biosynthesis studies, pantothenate kinase (PanK) research, and lipid metabolism experiments where endogenous pantothenate would confound results. Sodium pyruvate is also removed for researcher-defined carbon source control. FluxMPS™ delivers this triple-omission formulation with 0.04 µm filtration for OoC applications.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance ensures safe perfusion in every chip geometry, including sub-100 µm channels.

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Precise formulation control

Calcium-Free & Pantothenate-Free — defined omission enabling specific research applications that require exact control of this medium component.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm), USP <85> — eliminates ionic contaminants that interfere with specialized assays.

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Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin — prevents LPS-driven inflammatory signaling that would confound specialized cell culture outcomes.

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High-glucose energy support

4.5 g/L glucose sustains the energy demands of the cell types and protocols targeted by this formulation.

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Customization on demand

pH, glucose, salts, and additional component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma purity unavailable from conventional 0.22 µm filtered media.

1

0.1 µm Pre-filtration I — Large Particulate Removal

Removes large aggregates and contaminants; protects downstream 0.04 µm membranes and chip geometries from fouling.
2

0.04 µm Pre-filtration II — Mycoplasma Barrier

Retains mycoplasma (0.1–0.3 µm) and fine particulates — absent from standard 0.22 µm filtration.
3

0.1 µm Sterile-filtration I — Second-pass Redundancy

Second-pass sterility redundancy; no breakthrough from Stage 1.
4

0.04 µm Sterile-filtration II — Final Polish

Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-DMEMWOSPCCCP1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered DMEM, with a confirmed mycoplasma barrier at every production stage.

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size — sub-mycoplasma polishing

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier — USP <63>–equivalent assurance every lot.

FluxMPS™ DCP-DMEMWOSPCCCP1X Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/o Sodium Pyruvate, Calcium Chloride & D-Calcium Pantothenate: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Pre-filtration I, 0.04 μm Pre-filtration II mycoplasma barrier, 0.1 μm Sterile-filtration I, 0.04 μm Final Polish ? MPS-grade DMEM High Glucose Calcium-Free & Pantothenate-Free for organ-on-a-chip | Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for sub-mycoplasma purity.
© Diagnocine® — DCP-DMEMWOSPCCCP1X

Applications

Specialized applications requiring Calcium-Free & Pantothenate-Free

FluxMPS™ DCP-DMEMWOSPCCCP1X is designed for research protocols where calcium-free & pantothenate-free conditions are essential — combined with MPS-grade filtration purity for organ-on-a-chip and microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered variant available for automated bioreactor perfusion and robotic liquid handlers.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion circuits
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Microfluidics

Organ-on-a-Chip & MPS

Ultra-clean 0.04 µm filtration prevents microchannel clogging. Calcium-Free & Pantothenate-Free formulation delivered particle-free for specialized OoC protocols.

OoCMPSToCBoC

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Calcium-Free Cell Dissociation

FluxMPS™ DCP-DMEMWOSPCCCP1X delivers Calcium-Free & Pantothenate-Free conditions with sub-mycoplasma filtration purity for demanding calcium-free cell dissociation platforms.

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Calcium Signaling Studies

FluxMPS™ DCP-DMEMWOSPCCCP1X delivers Calcium-Free & Pantothenate-Free conditions with sub-mycoplasma filtration purity for demanding calcium signaling studies platforms.

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CoA Biosynthesis Research

FluxMPS™ DCP-DMEMWOSPCCCP1X delivers Calcium-Free & Pantothenate-Free conditions with sub-mycoplasma filtration purity for demanding coa biosynthesis research platforms.

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Pantothenate Kinase Assays

FluxMPS™ DCP-DMEMWOSPCCCP1X delivers Calcium-Free & Pantothenate-Free conditions with sub-mycoplasma filtration purity for demanding pantothenate kinase assays platforms.

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Lipid Metabolism Studies

FluxMPS™ DCP-DMEMWOSPCCCP1X delivers Calcium-Free & Pantothenate-Free conditions with sub-mycoplasma filtration purity for demanding lipid metabolism studies platforms.

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate \| [-] Sodium Pyruvate, [-] Calcium Chloride, [-] D-Calcium Pantothenate
Appearance	Red-colored, clear solution
Glucose	4500 mg/L (4.5 g/L, High Glucose)
Key feature	Calcium-Free & Pantothenate-Free
pH USP <791>	7.4 ± 0.04
Osmolality USP <785>	330.00–370.00 mOsm/kg H₂O
Total ingredients	29

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788>	NMT 25/mL
Particulate ≥25 µm USP <788>	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from manufacture
Shipping condition	Cold pack
CO₂ requirement	5% CO₂ required (sodium bicarbonate buffering)

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
Regulatory alignment	21 CFR Part 820 (cGMP) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

DMEM High Glucose — Calcium-Free & Pantothenate-Free: 29 ingredients verified per lot with CAS numbers for full raw-material traceability. Calcium chloride, D-calcium pantothenate, and sodium pyruvate are all absent. Add Ca²? (as CaCl₂ from a sterile stock) at defined concentrations for calcium re-supplementation experiments. Add D-pantothenate separately for CoA rescue experiments. Add sodium pyruvate (110 mg/L) for standard secondary carbon support.

Component	CAS Number	mg/L
INORGANIC SALTS
Ferric nitrate nonahydrate	7782-61-8	0.100
Magnesium sulphate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	3700.00
Sodium chloride	7647-14-5	6400.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	109.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	30.000
L-Arginine hydrochloride	1119-34-2	84.000
L-Cystine dihydrochloride	30925-07-6	62.570
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	105.000
L-Leucine	61-90-5	105.000
L-Lysine hydrochloride	657-27-2	146.000
L-Methionine	63-68-3	30.000
L-Phenylalanine	63-91-2	66.000
L-Serine	56-45-1	42.000
L-Threonine	72-19-5	95.000
L-Tryptophan	73-22-3	16.000
L-Tyrosine Disodium Salt dihydrate	69847-15-0	103.790
L-Valine	72-18-4	94.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	4.000
Folic acid	59-30-3	4.000
Nicotinamide	98-92-0	4.000
Pyridoxal hydrochloride	65-22-5	4.000
Riboflavin	83-88-5	0.400
Thiamine hydrochloride	67-03-8	4.00
i-Inositol	87-89-8	7.200
OTHERS
D-Glucose	50-99-7	4500.000
Phenol red sodium salt	34487-61-1	15.900

Custom formulation: Contact support@diagnocine.com for DCP-DMEMWOSPCCCP1X modifications — component additions, pH changes, or nutrient adjustments.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants that would interfere with specialized assay outcomes.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

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Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL per lot.

Particulate — USP <788> Method 2

Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: 330.00–370.00 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-DMEMWOSPCCCP1X lot at support@diagnocine.com.

Product Comparison

How DCP-DMEMWOSPCCCP1X compares

FluxMPS™ DCP-DMEMWOSPCCCP1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter	DCP-DMEMWOSPCCCP1X (FluxMPS™)	Conventional DMEM HG (0.22 µm filtered)	Standard DMEM HG (0.22 µm filtered)
High Glucose DMEM without Calcium Chloride, D-Calcium Pantothenate, and Sodium Pyruvate — for calcium-free, CoA-pathway, and defined-carbon research	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier	check_circle Yes (0.04 µm)	cancel No	cancel No
Endotoxin specification	< 0.05 EU/mL	NMT 1 EU/mL	NMT 1 EU/mL
USP <788> particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle MPS-grade	cancel Risk of clogging	cancel Risk of clogging
Custom formulation	check_circle Available	cancel Fixed	cancel Fixed

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMWOSPCCCP1X — DMEM High Glucose Calcium-Free & Pantothenate-Free.

DCP-DMEMWOSPCCCP1X is processed through a Quadruple-stage filtration system reaching 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, ToC, and LoC platforms. The Calcium-Free & Pantothenate-Free formulation is delivered with sub-mycoplasma filtration purity and < 0.05 EU/mL endotoxin for specialized cell culture applications on chip.

FluxMPS™ uses four sequential filters — 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish — resulting in approximately 5× fewer particles and confirmed mycoplasma retention at every production stage.

Three independent research needs drive these omissions: (1) Calcium chloride is removed for calcium-free protocols — enzymatic dissociation (trypsin, collagenase), calcium-imaging studies, TRPV channel research, and any protocol where extracellular Ca²? must be absent or precisely titrated. (2) D-Calcium pantothenate is removed for CoA pathway research — pantothenate is the precursor to coenzyme A (CoA) via the pantothenate kinase (PanK) pathway; its absence allows researchers to study CoA biosynthesis flux, PanK inhibitor efficacy, and acetyl-CoA-dependent lipid metabolism without confounding endogenous pantothenate. (3) Sodium pyruvate is removed for researcher-defined carbon supplementation. Together these three omissions create a uniquely controlled base for metabolic and signaling studies.

5% CO₂ required (sodium bicarbonate buffering).

Yes. Add FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or custom nutrients as required. Contact support@diagnocine.com for custom co-formulation.

FluxMPS™ DCP-DMEMWOSPCCCP1X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>) on every production lot — 20× below the 1 EU/mL common for cell culture grade reagents.

Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting the research applications of FluxMPS™ DCP-DMEMWOSPCCCP1X.

Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216–4220. doi:10.1073/pnas.77.7.4216
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/o Sodium Pyruvate, Calcium Chloride & D-Calcium Pantothenate: 1X Liquid