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FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/o Sodium Pyruvate, Calcium Chloride & D-Calcium Pantothenate: 1X Liquid

Product#: DCP-DMEMWOSPCCCP1X
$47.00
DCP-DMEMWOSPCCCP1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/o Sodium Pyruvate, Calcium Chloride & D-Calcium Pantothenate: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains Magnesium Contains High Glucose (4500 mg/L) Without Calcium Chloride Without Sodium Pyruvate

FluxMPS™ DCP-DMEMWOSPCCCP1X is a Microfluidics Suitable, quadruple-stage ultra-filtered DMEM High Glucose formulation manufactured without sodium pyruvate, calcium chloride, and D-calcium pantothenate — a triple-omission base built for calcium-signaling, CoA-biosynthesis, and defined-carbon research. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. D-Calcium Pantothenate is also absent, supporting coenzyme A biosynthesis and pantothenate kinase (PanK) research.

  • Calcium chloride–free — supports trypsin-based cell dissociation, calcium-signaling studies, and tight-junction permeability assays where extracellular Ca2+ must be controlled
  • D-Calcium pantothenate–free — enables coenzyme A (CoA) biosynthesis flux studies and pantothenate kinase (PanK) inhibitor research without confounding endogenous pantothenate
  • Sodium pyruvate–free — add pyruvate at a defined concentration for precise secondary carbon-source control
  • 0.04 µm final filtration — ultra-low particulate baseline suited to microfluidic channels below 100 µm
  • Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Ultrapure Type 1 water (18.2 MΩ·cm); manufactured under an ISO 13485:2016 quality management system with ISO Class 5 aseptic fill
  • Custom formulation modifications available on request — contact support@diagnocine.com
CAT. NO.
DCP-DMEMWOSPCCCP1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/o Sodium Pyruvate, Calcium Chloride & D-Calcium Pantothenate: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • Key featureTriple-omission: Calcium-Free, Pantothenate-Free & Pyruvate-Free
  • Formulation[+] Sodium Bicarbonate, [+] Phenol Red, [+] Magnesium, [+] High Glucose | [-] Calcium Chloride, [-] Sodium Pyruvate
  • AppearanceRed-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)330-370 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2-8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for calcium-sensitive assays and CoA pathway research

Removing calcium chloride and D-calcium pantothenate simultaneously addresses two distinct research needs: calcium-free conditions for trypsin-based cell dissociation, calcium-signaling studies, and tight-junction permeability assays; and pantothenate-free conditions for coenzyme A (CoA) biosynthesis, pantothenate kinase (PanK) research, and lipid metabolism experiments. Sodium pyruvate is also removed for researcher-defined carbon-source control. FluxMPS™ delivers this triple-omission formulation with 0.04 µm final filtration for microfluidic and organ-on-a-chip applications.

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Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance, supporting safe perfusion across microfluidic channel geometries, including sub-100 µm channels.

target

Precise metabolic control

Calcium-Free, Pantothenate-Free & Pyruvate-Free — a defined triple omission enabling researcher-controlled supplementation for calcium, CoA-pathway, and carbon-source studies.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic-carbon content for reproducible cell culture performance.

visibility

Low background for imaging

Ultra-low particulate baseline from 0.04 µm final filtration supports confocal microscopy and biosensor-based assays without particulate interference.

science

Rich, stable nutrient profile

Full complement of amino acids and B-vitamins at standard DMEM concentrations, released per lot with a complete Certificate of Analysis.

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Customization on demand

pH, glucose, salts, and additional component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter-plus-final-filter pairs, reach a final 0.04 µm polish under aseptic fill conditions — delivering ultra-low particulate purity beyond conventional 0.22 µm filtered media.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; provides 0.1 µm mycoplasma-retentive filtration (not tested per lot) and protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated 0.1 µm mycoplasma-retentive prefilter, protecting the second 0.04 µm cartridge and providing filtration redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate 0.04 µm polishing filter, followed by aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across four filtration stages
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMWOSPCCCP1X Dulbecco's Modified Eagle Medium DMEM High glucose without Sodium Pyruvate, Calcium Chloride and D-Calcium Pantothenate - Quadruple-stage filtration system 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable DMEM High Glucose Calcium-Free and Pantothenate-Free for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DMEMWOSPCCCP1X
Applications

Specialized applications requiring Calcium-Free, Pantothenate-Free & Pyruvate-Free media

FluxMPS™ DCP-DMEMWOSPCCCP1X is designed for research protocols where calcium-free and pantothenate-free conditions are essential, combined with Microfluidics Suitable filtration purity for organ-on-a-chip and microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor perfusion and robotic liquid handlers — see the Grade note above for how this differs from the Microfluidics Suitable line described on this page.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion circuits
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

hub

Organ-on-a-Chip & MPS

0.04 µm filtration reduces microchannel clogging risk. Calcium-Free, Pantothenate-Free & Pyruvate-Free formulation delivered at Microfluidics Suitable purity for OoC protocols.

OoCMPSToCBoC
science

Calcium-Free Cell Dissociation

Supports enzymatic dissociation protocols (trypsin, collagenase) requiring the absence of extracellular Ca2+.

analytics

Calcium Signaling Studies

Calcium-free base for TRPV channel research and calcium-imaging protocols requiring precisely titrated Ca2+ reintroduction.

biotech

CoA Biosynthesis Research

Pantothenate-free base supports coenzyme A biosynthesis flux studies without confounding endogenous pantothenate.

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Pantothenate Kinase Assays

Enables pantothenate kinase (PanK) inhibitor efficacy studies on a defined pantothenate-free background.

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Lipid Metabolism Studies

Supports acetyl-CoA-dependent lipid metabolism research on a controlled CoA-pathway background.

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, [+] Phenol Red, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] Calcium Chloride, [-] Sodium Pyruvate
Appearance Red-colored, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
Key feature Triple-omission: Calcium-Free, Pantothenate-Free & Pyruvate-Free
pH USP <791> 7.4
Osmolality USP <785> 330-370 mOsm/kg H2O
Total ingredients 29 ingredients across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> M1 NMT 25/mL
Particulate ≥25 µm USP <788> M1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Approximately 10% CO2 (calculated ~9.7% at pH 7.4 for 3700 mg/L sodium bicarbonate buffering)
Available pack sizes 500 mL, 1000 mL
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

DMEM High Glucose — Calcium-Free, Pantothenate-Free & Pyruvate-Free: 29 ingredients verified per lot with CAS numbers for full raw-material traceability. Calcium chloride, D-calcium pantothenate, and sodium pyruvate are all absent. Add Ca2+ (as CaCl2 from a sterile stock) at defined concentrations for calcium re-supplementation experiments. Add D-pantothenate separately for CoA rescue experiments. Add sodium pyruvate (110 mg/L) for standard secondary carbon support.

Component CAS Number mg/L
INORGANIC SALTS
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.900
Custom formulation: Contact support@diagnocine.com for DCP-DMEMWOSPCCCP1X modifications — component additions, pH changes, or nutrient adjustments.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm, with controlled trace-metal and organic-carbon content.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; a Certificate of Analysis is available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 330-370 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMWOSPCCCP1X compares

FluxMPS™ DCP-DMEMWOSPCCCP1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEMWOSPCCCP1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard DMEM HG
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
High Glucose DMEM without Calcium Chloride, D-Calcium Pantothenate & Sodium Pyruvate check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm, not tested per lot) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Method 1 tested cancel Not specified cancel Not specified
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Clogging risk cancel Clogging risk
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMWOSPCCCP1X — DMEM High Glucose, Calcium-Free, Pantothenate-Free & Pyruvate-Free.

Yes. DCP-DMEMWOSPCCCP1X is processed through a Quadruple-stage filtration system reaching 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, ToC, and LoC platforms. The Calcium-Free, Pantothenate-Free & Pyruvate-Free formulation is delivered at Microfluidics Suitable purity with an endotoxin release specification of < 0.05 EU/mL for specialized cell culture applications on chip.
 
Three independent research needs drive these omissions: (1) Calcium chloride is removed for calcium-free protocols — enzymatic dissociation (trypsin, collagenase), calcium-imaging studies, TRPV channel research, and any protocol where extracellular Ca2+ must be absent or precisely titrated; add CaCl2 from a sterile stock at your defined concentration. (2) D-Calcium pantothenate is removed for CoA pathway research — pantothenate is the precursor to coenzyme A (CoA) via the pantothenate kinase (PanK) pathway; its absence allows study of CoA biosynthesis flux, PanK inhibitor efficacy, and acetyl-CoA-dependent lipid metabolism without confounding endogenous pantothenate; add D-pantothenate separately for CoA rescue experiments. (3) Sodium pyruvate is removed for researcher-defined carbon supplementation; add at 110 mg/L for standard secondary carbon support.
Yes. This formulation is buffered with 3700 mg/L sodium bicarbonate. To maintain pH 7.4, an incubator atmosphere of approximately 10% CO2 (calculated ~9.7%) is required — higher than the 5% CO2 typical of lower-bicarbonate DMEM formulations.
Yes. Add FBS (typically 5-10%), serum-free supplements, growth factors, antibiotics, or custom nutrients as required. When adding serum or other protein-containing supplements, pre-filter through a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm filter for serum, as it will retain immunoglobulins, lipoproteins, and growth factors. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch. Every batch of DCP-DMEMWOSPCCCP1X is tested by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before release and must meet the specification of < 0.05 EU/mL. See the Product Comparison section for published supplier specifications.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting the research applications of FluxMPS™ DCP-DMEMWOSPCCCP1X.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  3. Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216-4220. doi:10.1073/pnas.77.7.4216
  4. Berridge MJ, Lipp P, Bootman MD. The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol. 2000;1:11-21. doi:10.1038/35036035
  5. Leonardi R, Zhang YM, Rock CO, Jackowski S. Coenzyme A: back in action. Prog Lipid Res. 2005;44:125-153. doi:10.1016/j.plipres.2005.04.001
  6. Rock CO, Calder RB, Karim MA, Jackowski S. Pantothenate kinase regulation of the intracellular concentration of coenzyme A. J Biol Chem. 2000;275:1377-1383. doi:10.1074/jbc.275.2.1377
  7. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  8. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j

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