FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o Folic Acid: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o Folic Acid: 1X Liquid
FluxMPS™ DCP-DMEMWOFA1X is an MPS-grade, ultra-filtered DMEM High Glucose — Folic Acid–Free formulation engineered for specialized research applications where precise formulation control is essential. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate | [-] Folic Acid.
- Folic acid–free — creates selective pressure for DHFR-mediated gene amplification in CHO-DHFR− cell lines
- Compatible with methotrexate (MTX) step selection for recombinant protein expression amplification
- All other High Glucose DMEM nutrients intact: L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate
- 0.04 µm final nano-filtration — sub-mycoplasma purity for microfluidic channels below 100 µm
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Endotoxin < 0.05 EU/mL (LAL, USP <85>)
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Custom formulation modifications available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- Key featureFolic Acid–Free
- Formulation[+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [-] Folic Acid
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)310.00–350.00 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months
Engineered for DHFR selection and folate metabolism research
Standard DMEM High Glucose contains folic acid — a substrate that cells can use to bypass dihydrofolate reductase (DHFR) dependency. Removing folic acid creates the selective pressure needed for DHFR-mediated gene amplification, methotrexate step selection, and any study where folate pathway activity must be controlled. FluxMPS™ delivers this folic acid–free formulation with 0.04 µm filtration and < 0.05 EU/mL endotoxin for use in CHO cell lines and microfluidic bioproduction models.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance ensures safe perfusion in every chip geometry, including sub-100 µm channels.
Precise formulation control
Folic Acid–Free — defined omission enabling specific research applications that require exact control of this medium component.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm), USP <85> — eliminates ionic contaminants that interfere with specialized assays.
Below TLR4 endotoxin threshold
< 0.05 EU/mL endotoxin — prevents LPS-driven inflammatory signaling that would confound specialized cell culture outcomes.
High-glucose energy support
4.5 g/L glucose sustains the energy demands of the cell types and protocols targeted by this formulation.
Customization on demand
pH, glucose, salts, and additional component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma purity unavailable from conventional 0.22 µm filtered media.
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1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates and contaminants; protects downstream 0.04 µm membranes and chip geometries from fouling.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) and fine particulates — absent from standard 0.22 µm filtration.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
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4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ DCP-DMEMWOFA1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered DMEM, with a confirmed mycoplasma barrier at every production stage.
© Diagnocine® — DCP-DMEMWOFA1X
Specialized applications requiring Folic Acid–Free
FluxMPS™ DCP-DMEMWOFA1X is designed for research protocols where folic acid–free conditions are essential — combined with MPS-grade filtration purity for organ-on-a-chip and microfluidic platform compatibility.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered variant available for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
- Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion circuits
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
Organ-on-a-Chip & MPS
Ultra-clean 0.04 µm filtration prevents microchannel clogging. Folic Acid–Free formulation delivered particle-free for specialized OoC protocols.
CHO Bioproduction
FluxMPS™ DCP-DMEMWOFA1X delivers Folic Acid–Free conditions with sub-mycoplasma filtration purity for demanding cho bioproduction platforms.
DHFR Gene Amplification
FluxMPS™ DCP-DMEMWOFA1X delivers Folic Acid–Free conditions with sub-mycoplasma filtration purity for demanding dhfr gene amplification platforms.
Methotrexate Selection
FluxMPS™ DCP-DMEMWOFA1X delivers Folic Acid–Free conditions with sub-mycoplasma filtration purity for demanding methotrexate selection platforms.
Recombinant Protein Expression
FluxMPS™ DCP-DMEMWOFA1X delivers Folic Acid–Free conditions with sub-mycoplasma filtration purity for demanding recombinant protein expression platforms.
Folate Metabolism Research
FluxMPS™ DCP-DMEMWOFA1X delivers Folic Acid–Free conditions with sub-mycoplasma filtration purity for demanding folate metabolism research platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate | [-] Folic Acid |
| Appearance | Red-colored, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| Key feature | Folic Acid–Free |
| pH USP <791> | 7.4 ± 0.04 |
| Osmolality USP <785> | 310.00–350.00 mOsm/kg H₂O |
| Total ingredients | 32 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ required (sodium bicarbonate buffering) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose — Folic Acid–Free: 32 ingredients verified per lot with CAS numbers for full raw-material traceability. Folic acid is intentionally absent. Do not add folic acid unless ending the selection period. All other vitamins, amino acids, and inorganic salts are present at standard DMEM High Glucose concentrations.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — eliminates ionic contaminants that would interfere with specialized assay outcomes.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay < 0.05 EU/mL per lot.
Particulate — USP <788> Method 2
Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Target: 310.00–350.00 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-DMEMWOFA1X compares
FluxMPS™ DCP-DMEMWOFA1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMWOFA1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG (0.22 µm filtered) |
|---|---|---|---|
| High Glucose DMEM without Folic Acid — DHFR selection, gene amplification, and folate metabolism studies | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMWOFA1X — DMEM High Glucose Folic Acid–Free.
Supporting literature
Key publications supporting the research applications of FluxMPS™ DCP-DMEMWOFA1X.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216–4220. doi:10.1073/pnas.77.7.4216
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
