FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ DCP-DMEM-QPBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose (4.5 g/L) formulation engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and energy-demanding cell models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Calcium, [+] Magnesium, [+] Glucose (4500 mg/L, High Glucose) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate.

• High Glucose (4.5 g/L / 4500 mg/L) supports energy-demanding cell types — primary neurons, cardiomyocytes, and cancer lines — supplied without added glutamine, sodium pyruvate, sodium bicarbonate, or phenol red
• Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off suited to microfluidic channels below 100 µm
• Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>), tested per manufacturing batch, not per unit
• Bicarbonate-free and phenol red–free formulation, compatible with Agilent Seahorse XF metabolic flux assays that require both
• Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon (TOC) content
• Manufactured under an ISO 13485:2016 quality management system; final QC at the Diagnocine R&D Center, Totowa, NJ
• 29 verified ingredients across inorganic salts, amino acids, and vitamins, at 4× Basal Medium Eagle (BME) amino acid/vitamin concentration
• Custom pH, glucose concentration, salts, HEPES, and nutrient adjustments available on request — contact support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized organisms, subvisible particulates, and cellular debris that clog microfluidic channels and interfere with sensor signals. FluxMPS™ High Glucose is filtered to a 0.04 µm final cut-off while delivering 4.5 g/L glucose for energy-demanding cell types and complex tissue models.

Quadruple-stage filtration system

Four filtration passes, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions.

1. 1

   0.1 µm Prefiltration I

   Removes large aggregates, protein aggregates and cell debris; protects the first 0.04 µm cartridge from early fouling.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and organisms in the mycoplasma size range (0.2–0.3 µm) that pass a conventional 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   A second, dedicated prefilter protecting the second 0.04 µm cartridge — full redundancy, not a polish of Stage 2 effluent.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill & finish in an ISO Class 5 (Class 100) environment.

Designed for next-generation cell models

FluxMPS™ DCP-DMEM-QPBR1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. High glucose (4.5 g/L) makes it particularly suited to energy-intensive cell types and aerobically active cultures.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available on request for automated bioreactor perfusion and robotic liquid handlers.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and optical sensors
• Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. Available pack sizes: 500 mL, 1000 mL. CoA: support@diagnocine.com.

Full composition (mg/L)

DMEM High Glucose is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations, plus glycine, serine, and ferric nitrate. 29 ingredients verified per lot with CAS numbers for raw-material traceability.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-DMEM-QPBR1X compares

FluxMPS™ DCP-DMEM-QPBR1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEM-QPBR1X DMEM High Glucose.

Supporting literature

Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable DMEM High Glucose in organ-on-a-chip and metabolic research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j