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FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-DMEM-QPR1X
$44.00
DCP-DMEM-QPR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains Calcium Contains Magnesium Contains High Glucose Without L-Glutamine Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-DMEM-QPR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose (4.5 g/L) formulation engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and energy-demanding cell models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate.

  • High Glucose (4.5 g/L / 4500 mg/L) formulation supports energy-demanding cell types — primary neurons, cardiomyocytes, and cancer lines
  • Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final cut-off for microfluidic channel safety
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Formulated without L-glutamine, sodium pyruvate, and phenol red for a clean optical baseline and flexible fresh-supplementation of metabolic substrates
  • Manufactured under an ISO 13485:2016 quality management system; final QC and release testing at Diagnocine, Totowa, NJ
  • Ultrapure Type 1 water (18.2 MΩ·cm) used in formulation, per lot
  • 31 verified ingredients across inorganic salts, amino acids, and vitamins, with full raw-material CAS traceability
  • Custom pH, glucose concentration, salts, and nutrient adjustments available on request — contact support@diagnocine.com
CAT. NO.
DCP-DMEM-QPR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid
  • Formulation[+] NaHCO3, Ca, Mg, High Glucose | [-] L-Gln, Phenol Red, Na-Pyruvate
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • AppearancePale yellow to colorless, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)320–360 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • Shipping ConditionCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized organisms, subvisible particulates, and aggregate fragments that clog microfluidic channels and corrupt sensor signals. FluxMPS™ High Glucose reduces these failure modes while delivering 4.5 g/L glucose for energy-demanding cell types and complex tissue models.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> Method 1 (light obscuration) particulate compliance support safe perfusion in fine chip geometries, including sub-100 µm channels.

bolt

High-energy cell support

4.5 g/L glucose (4× vs. low-glucose DMEM) sustains primary neurons, cardiomyocytes, cancer lines, and other aerobically active cells in prolonged perfusion.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) with rigorous trace-metal and total organic carbon (TOC) control supports demanding cell models and sensitive analytical workflows.

visibility

Low background for imaging

Ultra-low particulate baseline from 0.04 µm final filtration, combined with a phenol red–free formulation, supports clean particulate baselines for confocal microscopy and biosensor platforms.

science

Rich, stable nutrient profile

31 ingredients verified per lot; formulated to 4× Basal Medium Eagle (BME) amino acid and vitamin concentrations; micro-batch production with full traceability.

tune

Customization on demand

pH, glucose, salts, HEPES, and nutrients adjustable. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated 0.1 µm prefilter / 0.04 µm final-filter pairs run in series — reach a validated 0.04 µm final cut-off, purpose-built for microfluidic channel geometries below 100 µm.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and particulate contaminants; protects the first 0.04 µm final filter cartridge from early fouling.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a standard 0.22 µm filter does not capture.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge and providing full-train redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing pass; aseptic fill and finish in a validated ISO Class 5 (Class 100) laminar-flow environment.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across a 4-stage filtration train
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (mycoplasma organisms range 0.2–0.3 µm in diameter) at multiple stages of the train; lots are not individually tested for mycoplasma.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEM-QPR1X Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid ? quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish ? Microfluidics Suitable DMEM High Glucose for organ-on-a-chip and microphysiological systems | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) delivering a 0.04 µm final cut-off for microfluidic-safe purity.
© Diagnocine® — DCP-DMEM-QPR1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEM-QPR1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. High glucose (4.5 g/L) makes it particularly suited to energy-intensive cell types and aerobically active cultures.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for automated bioreactor perfusion and robotic liquid handlers — see the Grade note above for how this tier differs from the standard Microfluidics Suitable formulation.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the standard 0.04 µm train
  • Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and optical sensors
  • Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Organ-on-a-Chip & MPS

Ultra-clean 0.04 µm–filtered media supports uninterrupted flow in complex multi-organ chip architectures.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Cancer Models

High glucose (4.5 g/L) supports aerobic glycolysis phenotyping in cancer cell lines requiring elevated energy substrates.

MCF-7MDA-MB-231HeLaA549
Neuroscience

Primary Neurons & Brain-on-Chip

High glucose supports the metabolic demands of primary neurons and iPSC-derived neuronal networks in microfluidic perfusion.

iPSC-NeuronsPrimary neuronsBrain-on-chip
Cardiac Biology

Cardiomyocyte & Heart-on-Chip

High-glucose DMEM is a standard base for iPSC-derived cardiomyocyte maturation and heart-on-chip functional assays.

iPSC-CMHeart-on-chipTEER
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation supports 13C isotope tracing and NMR metabolomics profiling of glycolytic flux. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate levels and a phenol red–free formulation support clean baselines for confocal microscopy and biosensor platforms.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot is released against the full specification matrix below. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate
Appearance Pale yellow to colorless, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
pH USP <791> 7.4
Osmolality USP <785> 320–360 mOsm/kg H2O
L-Glutamine Not added — supplement fresh at time of use
Sodium Pyruvate Not added
Phenol Red Not added
Total ingredients 31
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100) laminar flow
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement ≈9.6% CO2 (derived from 3700 mg/L NaHCO3 to maintain pH 7.4; verify against incubator set point)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

DMEM High Glucose is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations, plus glycine, serine, and ferric nitrate. 31 ingredients verified per lot with CAS numbers for raw-material traceability.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 584.000
L-Isoleucine 73-32-5 42.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 105.000
L-Methionine 63-68-3 146.000
L-Phenylalanine 63-91-2 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 4500.000
Custom formulation: Contact support@diagnocine.com for DCP-DMEM-QPR1X custom specifications — pH, glucose, salts, HEPES, or nutrient modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC and release testing performed at Diagnocine’s R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm resistivity with trace-metal and total organic carbon (TOC) control, supporting sensitive cell models and analytical workflows.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, ensuring container-closure integrity.

assignment

Micro-Batch Precision

Small-batch production with full per-lot traceability and a Certificate of Analysis for every lot — no blending, no averaged QC results.

Endotoxin — USP <85> BET

LAL assay; release specification: < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 320–360 mOsm/kg H2O.

Documentation & CoA

Full Certificate of Analysis with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEM-QPR1X compares

FluxMPS™ DCP-DMEM-QPR1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEM-QPR1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard Alt. DMEM HG
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm final cut-off) Standard grade (0.22 µm) Standard grade (0.22 µm)
High Glucose DMEM without L-Glutamine, Pyruvate, and Phenol Red — imaging-ready with flexible metabolic supplementation check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stages) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Ultrapure Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEM-QPR1X DMEM High Glucose.

Yes. DCP-DMEM-QPR1X is Microfluidics Suitable, processed through a quadruple-stage filtration system (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off, which supports safe perfusion in microphysiological systems (MPS), organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and lab-on-a-chip (LoC) platforms. High glucose (4.5 g/L) supports energy-demanding cell types including primary neurons, cardiomyocytes, and cancer cell lines in long-duration chip perfusion.
FluxMPS™ uses four sequential filtration stages arranged as two prefilter-and-final-filter pairs: 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish). Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter, giving full redundancy across two complete passes.
Phenol red is excluded to provide a clean optical baseline for imaging and biosensor applications. L-glutamine and sodium pyruvate are excluded so you can add them fresh at the concentration your protocol requires (L-glutamine degrades in liquid storage over time). Add L-glutamine (typically 2–4 mM) and/or sodium pyruvate (typically 1 mM) at time of use, or use a stabilized dipeptide form of glutamine for extended culture.
Yes. This formulation contains sodium bicarbonate (3700 mg/L, ≈44 mM) as its buffering system. To maintain the target pH of 7.4, this bicarbonate concentration corresponds to approximately 9.6% CO2 (derived via the Henderson–Hasselbalch equation), not the 5% CO2 level associated with lower-bicarbonate formulations. Verify your incubator CO2 set point against this calculated value, or contact us for a HEPES-buffered, reduced-bicarbonate alternative.
Yes. Fetal bovine serum (typically 5–10%), serum-free supplements, growth factors, and antibiotics can all be added. When adding serum or other protein-containing supplements, filter using a 0.2 µm low-protein-binding PES or PVDF membrane — never a 0.04 µm membrane, which will retain immunoglobulins, lipoproteins, and other serum components the culture needs. Contact support@diagnocine.com for custom co-formulation.
Each manufacturing batch is tested by LAL assay (USP <85>, Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet a release specification of < 0.05 EU/mL before release. This is a batch-level release specification, not a per-unit certificate — see the Manufacturing & Compliance section for the full batch quality control program.
Yes. A full CoA is available per lot and covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control, particulate count (USP <788>, Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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