FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine: 1X Liquid

FluxMPS™ DMEM, High Glucose, w/o L-Glutamine — 1X Liquid Cell Culture Medium

FluxMPS™ DCP-DMEM-Q1X is an MPS-grade, ultra-filtered Dulbecco’s Modified Eagle Medium (DMEM) formulated with 4.5 g/L high glucose and sodium bicarbonate, without L-Glutamine — engineered specifically for microfluidic channels, organ-on-a-chip (OoC), and microphysiological system (MPS) platforms. Purified through a proprietary Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it is approximately 5× cleaner than conventional 0.22 µm-filtered DMEM by particulate count, delivering sub-mycoplasma polishing and ultra-low endotoxin (< 0.05 EU/mL) for the most demanding perfusion and live-imaging applications.

• Quadruple-stage nano-filtration: 0.1 µm ×2 + 0.04 µm ×2 for sub-mycoplasma polishing and ultra-low particulate load
• Endotoxin < 0.05 EU/mL verified by USP <85> Limulus Amebocyte Lysate (LAL) assay
• 4× BME amino acid & vitamin concentration — rich, stable nutrient base for demanding cell types
• L-Glutamine-free formulation: add exogenous L-Glutamine or GlutaMAX™ per your experimental protocol
• [+] High Glucose (4,500 mg/L)  ·  [+] Sodium Bicarbonate  ·  [+] Sodium Pyruvate  ·  [+] Phenol Red  ·  [−] L-Glutamine·  [−] HEPES
• Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) — no ionic contaminants interfering with biosensors or TEER
• ISO Class 5 (Class 100) aseptic fill & finish — microchannel-safe, particle-tested per USP <788>
• Customizable: pH, glucose, HEPES, salts, and nutrient concentrations available on request — support@diagnocine.com

Engineered where standard DMEM fails

Conventional 0.22 µm-filtered DMEM is designed for conventional well-plate culture, not for the microscale geometries of organ-on-a-chip devices. Residual mycoplasma-sized particles (< 0.22 µm) accumulate in microchannels, corrupt TEER measurements, trigger non-specific inflammatory signals, and shorten perfusion device lifespan. FluxMPS™ DCP-DMEM-Q1X uses a Quadruple-stage filtration architecture that eliminates this failure mode while preserving the rich 4× BME amino acid and vitamin profile that DMEM is valued for.^[1,2]

Quadruple-stage filtration system

FluxMPS™ DCP-DMEM-Q1X is the only ready-to-use 0.04 µm-final-filtered DMEM variant at this purity level. The proprietary four-stage filtration train removes large aggregates, bacteria, mycoplasma, and sub-micron particulates in sequential passes before aseptic ISO Class 5 fill — delivering approximately 5× fewer subvisible particles than conventional 0.22 µm-filtered DMEM.

1. 1

   0.1 µm Pre-filtration I — Large Particulate & Aggregate Removal

   First 0.1 µm membrane pass captures large aggregates, cell debris, and coarse particulates, extending the service life of downstream membranes and establishing a clean base stream for subsequent polishing stages.

2. 2

   0.04 µm Pre-filtration II — Fine Particulate, Bacteria & Mycoplasma Retention

   The first 0.04 µm (40 nm) membrane provides sub-mycoplasma barrier filtration, retaining organisms such as Acholeplasma laidlawii (300–800 nm) and fine inorganic particulates that pass the 0.22 µm stage.

3. 3

   0.1 µm Sterile-filtration I — Second-Pass Redundancy

   A second independent 0.1 µm membrane provides sterile-filtration redundancy, catching any upstream shed particles and confirming bioburden reduction before the final polishing stage.

4. 4

   0.04 µm Sterile-filtration II — Final Polish & ISO Class 5 Aseptic Fill

   The final 0.04 µm membrane delivers ultimate particulate polishing. Media passes directly into ISO Class 5 (Class 100) laminar-flow fill suites, ensuring sterility and ultra-low particle counts confirmed per USP <788> Method 2.

Purpose-built for microphysiological & metabolic research

FluxMPS™ DCP-DMEM-Q1X is the preferred basal medium for researchers requiring a clean, defined, L-Glutamine-free DMEM base for organ-on-a-chip (OoC), microfluidic perfusion, Warburg-effect metabolomics, and any application where particle-induced artifact or glutamine-derived ammonia must be eliminated from the system.^[4,5]

Automated Bioreactors & Robotics

For automated perfusion bioreactors and robotic liquid-handling systems with sub-10 µm valve tolerances, Diagnocine offers an optional ultra-filtered 0.01 µm (10 nm) variant of FluxMPS™ DMEM. This nano-grade format virtually eliminates all particulate species that could foul microvalves, flow sensors, or acoustic fluidic actuators over extended runs.

• Total Particulate Exclusion: 10 nm final filtration eliminates virtually all particle species — protecting microvalves and sensors in automated organ-on-a-chip workstations
• Valve & Sensor Protection: Reduces actuator wear in PDMS and thermoplastic chip platforms with channel cross-sections below 50 µm
• Extended Perfusion Stability: Maintains defined nutrient composition across 7–21-day continuous perfusion runs without particulate-driven drift

Inquiry Required: The 0.01 µm (10 nm) ultra-filtered grade is available on special order. Contact support@diagnocine.com to request this format for your automated system.

Full QC specification suite

Every lot of FluxMPS™ DCP-DMEM-Q1X is tested against the full specification suite below before release. Certificate of Analysis (CoA) is available on request.

Full composition (mg/L)

FluxMPS™ DCP-DMEM-Q1X is based on the classical DMEM formulation featuring 4× BME amino acid and vitamin concentrations. The composition below reflects the per-lot release basis. L-Glutamine, Sodium Pyruvate, and Phenol Red are excluded; add 2–4 mM L-Glutamine or equivalent GlutaMAX™ per your protocol.

ISO 13485-certified manufacturing & compliance

FluxMPS™ DCP-DMEM-Q1X is manufactured in Totowa, NJ, USA under an ISO 13485:2016-certified Quality Management System with full micro-batch traceability and multi-standard QC release testing.

How DCP-DMEM-Q1X compares

FluxMPS™ DCP-DMEM-Q1X vs. conventional 0.22 µm-filtered DMEM variants — across the parameters that matter most for microphysiological systems and microfluidic applications.

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEM-Q1X and its use in microphysiological, microfluidic, and metabolic research.

Supporting literature

Key peer-reviewed references supporting the use of ultra-filtered DMEM in organ-on-a-chip, microfluidic perfusion, and metabolic research applications.

1. Bhatia, S.N. & Ingber, D.E. Microfluidic organs-on-chips. Nature Biotechnology 32, 760–772 (2014). doi:10.1038/nbt.2989
2. Huh, D. et al. Reconstituting organ-level lung functions on a chip. Science 328, 1662–1668 (2010). doi:10.1126/science.1188302
3. Dulbecco, R. & Freeman, G. Plaque production by the polyoma virus. Virology 8, 396–397 (1959). doi:10.1016/0042-6822(59)90043-1
4. Warburg, O. On the origin of cancer cells. Science 123, 309–314 (1956). doi:10.1126/science.123.3191.309
5. Sung, J.H. et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab on a Chip 13, 1201–1212 (2013). doi:10.1039/c3lc41017j
6. Bhattacharya, S. et al. Validation of USP <788> particulate matter testing for cell culture media used in bioreactor manufacturing. PDA Journal of Pharmaceutical Science and Technology 75, 202–215 (2021). doi:10.5731/pdajpst.2020.011940
7. Zhang, Y.S. et al. Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS 114, E2293–E2302 (2017). doi:10.1073/pnas.1612906114
8. Niklison-Chirou, M.V. et al. How does p53 induce apoptosis and how does this relate to p53-mediated tumour suppression? Cell Death & Differentiation 28, 983–996 (2021). doi:10.1038/s41418-021-00760-1
9. Campisi, M. et al. 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes. Biomaterials 180, 117–129 (2018). doi:10.1016/j.biomaterials.2018.07.014
10. Yin, X. et al. Engineering stem cell organoids. Cell Stem Cell 18, 25–38 (2016). doi:10.1016/j.stem.2015.12.005