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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEMH-QPBR1X is a Microfluidics Suitable, ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and CO₂-independent or open-top microfluidic platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO₂-independent pH buffering. Formulation: [+] HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate.
- High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and other aerobically active cells
- 25 mM HEPES (pKa 7.3 at 37°C) — pH buffering independent of CO₂ tension
- 0.04 µm final filtration — pore size below the mycoplasma diameter range (0.2–0.3 µm), suited to microfluidic channels under 100 µm
- Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
- Endotoxin < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 aseptic fill
- Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
- Formulation[+] HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate
- AppearancePale yellow colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that can accumulate in microfluidic channels. Standard DMEM also lacks HEPES buffering, causing pH instability whenever chips are handled outside incubators. FluxMPS™ addresses both failure modes.
Microchannel-safe purity
0.04 µm final filtration reduces particulate size below typical microchannel dimensions. USP <788> Method 1 (light obscuration) confirms the finished product meets subvisible particulate limits.
High-energy cell support
4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — through long-duration perfusion.
HEPES: CO₂-stable pH
25 mM HEPES (pKa 7.3 at 37°C) helps maintain pH in the 7.2–7.4 range independent of incubator CO₂ — useful for open-top chips, point-of-care devices, and atmospheric incubation.
Low background for imaging
Low particulate baseline supports high-contrast imaging; the phenol red–free formulation avoids background absorbance in colorimetric assays. Suited to confocal microscopy and optical biosensor platforms on chip.
Rich, stable nutrient profile
30 ingredients verified per batch; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.
Customization on demand
pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two dedicated 0.1 µm/0.04 µm prefilter-and-final-filter pairs in series — reaching a final 0.04 µm polish, delivering low-particulate purity for microfluidic channel applications.
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1
0.1 µm Prefiltration I
Removes large aggregates, cell debris, and mycoplasma-sized particles (0.2–0.3 µm) via 0.1 µm mycoplasma-retentive filtration (not tested per lot); protects the first 0.04 µm cartridge.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that a standard 0.22 µm filter would not capture.
-
3
0.1 µm Prefiltration II
Second dedicated 0.1 µm mycoplasma-retentive prefilter, protecting the second 0.04 µm cartridge.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-DMEMH-QPBR1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMH-QPBR1X combines high-glucose energy support with HEPES pH stability — supporting platforms from open-top microfluidic chips and CO₂-free bioreactors to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
HEPES buffering removes CO₂ dependency during automated bioreactor perfusion. An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for robotic liquid handlers where trace particulates cause valve failure.
- Total Particulate Exclusion: the 0.01 µm MPS Grade variant removes nanoparticulate aggregates from bioreactor media lines
- pH-Stable Automated Delivery: HEPES maintains pH during robotic media exchanges without CO₂ re-equilibration delays
- Extended Perfusion Stability: consistent high-glucose delivery with stable pH over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Open-Top & CO₂-Free Chips
HEPES buffering enables pH-stable culture in open-top microfluidic devices, atmospheric incubators, and multi-compartment chips with heterogeneous CO₂ environments.
Primary Neurons & Brain-on-Chip
High glucose (4.5 g/L) plus HEPES stability supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion without pH drift.
Cardiomyocyte & Heart-on-Chip
High-glucose DMEM plus HEPES is a common base for iPSC-CM maturation and heart-on-chip functional assays requiring stable pH and high energy substrate.
Warburg Effect & Cancer Models
High glucose supports aerobic glycolysis in cancer lines; HEPES helps stabilize pH during rapid glucose-consumption spikes in Warburg-active tumor models.
Metabolic Flux Analysis
Defined high-glucose formulation for ¹³C isotope tracing and Seahorse XF assays; HEPES allows sampling outside incubators without pH artefacts.
Microscopy & Optical Sensing
HEPES-stabilized pH during imaging sessions; the phenol red–free formulation avoids background absorbance for colorimetric and fluorescence-based readouts.
Analytical release specifications
Every batch released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate |
| Appearance | Pale yellow colored, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H2O |
| Total ingredients | 30 |
| Pack sizes | 500 mL, 1000 mL |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent — HEPES (25 mM) alone maintains pH without gas supplementation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 30 ingredients verified per batch with CAS numbers for full raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the OTHERS group.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 4500.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QA and testing at the Diagnocine R&D Center, Totowa, NJ, USA.
Trace-Metal & TOC-Controlled Water
Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic-carbon (TOC) content supports consistent raw-material quality.
ISO Class 5 Fill & Finish
Aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation, supporting container-closure integrity.
Micro-Batch Precision
Small-batch production with full per-lot traceability; a Certificate of Analysis is available for every batch.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 310–350 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every batch on request.
How DCP-DMEMH-QPBR1X compares
FluxMPS™ DCP-DMEMH-QPBR1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMH-QPBR1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG + HEPES (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not designated | Not designated |
| High Glucose + 25mM HEPES, bicarbonate-free & phenol red–free | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm, not tested per lot) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes (Method 1) | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Yes | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMH-QPBR1X DMEM High Glucose + 25mM HEPES.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j