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FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM) (ATCC Modification) w/o Phenol Red: 1X Liquid

Product#: DCP-DMEMA-R1X
$52.80
DCP-DMEMA-R1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM) (ATCC Modification) w/o Phenol Red: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Phenol Red

FluxMPS™ DCP-DMEMA-R1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose — ATCC Modification — Phenol Red-Free formulation, engineered for research protocols where ATCC-validated formulation control and microfluidic-grade purity are both required. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] 4.5 g/L Glucose, [+] L-Glutamine, [+] 1 mM Sodium Pyruvate, [+] 1.5 g/L Sodium Bicarbonate, [+] Calcium, [+] Magnesium | [-] Phenol Red.

  • ATCC Modification: 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate, matching ATCC’s validated formulation for its authenticated reference cell line panel
  • Phenol red-free formulation removes the dye’s own visible-light absorbance, supporting fluorescence-based assays and ER-positive cell line work
  • Compatible with ATCC authentication protocols for HeLa, MCF-7, A549, HEK-293, and Vero reference lines
  • Quadruple-stage filtration (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm final polish) reaching a 0.04 µm final pore size for microfluidic channel compatibility
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • 5% CO2 incubation recommended, consistent with the 1.5 g/L sodium bicarbonate buffering system
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom formulation modifications (pH, glucose, salts) available on request
CAT. NO.
DCP-DMEMA-R1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium (DMEM) (ATCC Modification) w/o Phenol Red: 1X Liquid
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Glucose (4.5 g/L), [+] Sodium Pyruvate (1 mM) | [-] Phenol Red
  • AppearanceColorless (phenol red-free), clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • CO2 Requirement5% CO2 required (sodium bicarbonate-buffered)
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

The ATCC-specified formulation for authenticated cell line culture

ATCC (American Type Culture Collection) maintains specific media formulations validated against its authenticated reference cell lines. The ATCC Modification of DMEM uses precisely 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate — concentrations validated for ATCC-maintained cell lines including HeLa, MCF-7, A549, HEK-293, and Vero. Removing phenol red eliminates the dye’s own absorbance for fluorescence assays and hormone-sensitive cell lines. FluxMPS™ adds quadruple-stage 0.04 µm filtration to enable use on microfluidic and organ-on-a-chip platforms.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in chip geometries, including sub-100 µm channels.

science

ATCC-validated formulation

1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate match ATCC’s specification for its authenticated reference cell line panel.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) supports trace-metal and organic-carbon (TOC) control for sensitive analytical and imaging assays.

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Low background for imaging

Phenol red-free formulation removes the pH indicator’s own visible-light absorbance interference. Combined with 0.04 µm final filtration, particulate baseline is minimized for confocal imaging and biosensor applications.

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High-glucose energy support

4.5 g/L glucose plus 1 mM sodium pyruvate sustain the energy demands of glycolytically active and OXPHOS-dependent reference cell lines.

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Customization on demand

pH, glucose, salts, and additional component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma-retentive purity beyond conventional 0.22 µm filtered media.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2 effluent, but redundancy for Stage 4.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish in ISO Class 5 (Class 100) laminar-flow conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across two filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm mycoplasma-retentive filtration (mycoplasma diameter 0.2–0.3 µm); lots are not individually tested to USP <63>.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMA-R1X Dulbecco's Modified Eagle Medium (DMEM) (ATCC Modification) w/o Phenol Red: 1X Liquid, Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish, Microfluidics Suitable DMEM High Glucose ATCC Modification Phenol Red-Free for organ-on-a-chip, Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for sub-mycoplasma-retentive purity.
© Diagnocine® — DCP-DMEMA-R1X
Applications

Specialized applications requiring ATCC Modification — Phenol Red-Free

FluxMPS™ DCP-DMEMA-R1X is designed for research protocols where ATCC Modification — Phenol Red-Free conditions are essential, combined with Microfluidics Suitable filtration purity for organ-on-a-chip and microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available for automated bioreactor perfusion and robotic liquid handlers — see the Grade note in the filtration section above.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
  • Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion circuits
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

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Organ-on-a-Chip & MPS

Ultra-clean 0.04 µm filtration prevents microchannel clogging. ATCC Modification — Phenol Red-Free formulation delivered particle-controlled for specialized OoC protocols.

OoCMPSToCBoC
science

HeLa Culture

Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for HeLa reference-line culture.

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MCF-7 & Cancer Lines

Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for MCF-7 and related cancer cell line platforms.

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HEK-293 Expression

Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for HEK-293 expression platforms.

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Vero Cell Culture

Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for Vero cell culture platforms.

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ATCC-Protocol Compatible

Delivers ATCC Modification — Phenol Red-Free conditions with mycoplasma-retentive filtration purity for ATCC-protocol compatible research platforms.

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Glucose (4.5 g/L), [+] Sodium Pyruvate (1 mM) | [-] Phenol Red
Appearance Colorless (phenol red-free), clear solution
Glucose 4500 mg/L (4.5 g/L, per ATCC specification)
L-Glutamine 584 mg/L
Sodium Pyruvate 110 mg/L (1 mM)
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 32 ingredients across 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 browsable tabs
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (see § Manufacturing & Compliance for batch-release testing)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 required (derived from 1.5 g/L sodium bicarbonate)
Pack sizes available 500 mL, 1000 mL
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

DMEM High Glucose — ATCC Modification — Phenol Red-Free: 32 ingredients verified per lot with CAS numbers for full raw-material traceability. ATCC Modification formulation: 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate. Phenol red is absent. These concentrations are validated for ATCC-maintained cell lines — do not substitute standard DMEM for ATCC-protocol experiments.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 1500.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 4500.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-DMEMA-R1X modifications — component additions, pH changes, or nutrient adjustments.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm feed water — supports trace-metal and organic-carbon (TOC) control for sensitive assay outcomes.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-DMEMA-R1X lot at support@diagnocine.com.
Product Comparison

How DCP-DMEMA-R1X compares

FluxMPS™ DCP-DMEMA-R1X vs. conventional 0.22 µm-filtered DMEM High Glucose formulations.

Parameter DCP-DMEMA-R1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard DMEM HG
(0.22 µm filtered)
Grade Microfluidics Suitable Standard grade (no MPS designation) Standard grade (no MPS designation)
DMEM ATCC Modification — 1.5 g/L bicarbonate validated for ATCC reference cell lines, phenol red-free for imaging check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm mycoplasma-retentive) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMA-R1X — DMEM High Glucose ATCC Modification — Phenol Red-Free.

Yes. DCP-DMEMA-R1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels for MPS, OoC, ToC, and LoC platforms. The ATCC Modification — Phenol Red-Free formulation is delivered with mycoplasma-retentive filtration purity and an endotoxin release specification of < 0.05 EU/mL for specialized cell culture applications on chip. It is a Microfluidics Suitable (0.04 µm) product, not an MPS Grade (0.01 µm) product.
 
The ATCC Modification uses precisely 1.5 g/L sodium bicarbonate (vs. 3.7 g/L in standard high-glucose DMEM) and 1 mM sodium pyruvate — concentrations specified by ATCC for its authenticated reference cell line panel, designed for 5% CO2 incubation. Phenol red is removed to eliminate the dye’s own visible-light absorbance, making this the preferred base for fluorescence-based assays and ER-positive cell line work. No component has been substituted or supplemented beyond what is listed in the composition table.
Yes. This formulation is buffered with 1.5 g/L sodium bicarbonate and requires 5% CO2 incubation to maintain pH 7.4. It is not HEPES-buffered and is not CO2-independent.
Yes. Add FBS (typically 5-10%), serum-free supplements, growth factors, or antibiotics as required. When filtering serum-containing or protein-containing additions, use a 0.2 µm low-protein-binding PES or PVDF filter — never 0.04 µm, which retains IgM, VLDL, and much of the lipid/lipoprotein fraction of serum. Contact support@diagnocine.com for custom co-formulation.
Every DCP-DMEMA-R1X batch is tested by LAL assay (USP <85>) and must meet the release specification of < 0.05 EU/mL before release. Endotoxin is controlled per manufacturing batch, not per individual unit.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting the research applications of FluxMPS™ DCP-DMEMA-R1X.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  3. Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216-4220. doi:10.1073/pnas.77.7.4216
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  6. Lincoln B, et al. ATCC animal cell culture guide: reference cell line authentication and quality control. ATCC Technical Bulletin. 2019. atcc.org
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446-452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j

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