FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM) (ATCC Modification) w/o Phenol Red: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM) (ATCC Modification) w/o Phenol Red: 1X Liquid

FluxMPS™ DCP-DMEMA-R1X is an MPS-grade, ultra-filtered DMEM High Glucose — ATCC Modification — Phenol Red–Free formulation engineered for specialized research applications where precise formulation control is essential. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] 4.5 g/L Glucose, [+] L-Glutamine, [+] 1 mM Sodium Pyruvate, [+] 1.5 g/L Sodium Bicarbonate | [-] Phenol Red.

• ATCC Modification: 1.5 g/L sodium bicarbonate & 1 mM sodium pyruvate — validated for ATCC reference cell lines
• Phenol red–free: zero autofluorescence (520–560 nm) and no estrogen-receptor agonist activity
• Compatible with ATCC authentication protocols for HeLa, MCF-7, A549, HEK-293, Vero, and other ATCC lines
• 0.04 µm final nano-filtration — sub-mycoplasma purity for microfluidic channels below 100 µm
• Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
• Endotoxin < 0.05 EU/mL (LAL, USP <85>)
• Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
• Custom formulation modifications available on request

The ATCC-specified formulation for authenticated cell line culture

ATCC (American Type Culture Collection) maintains specific media formulations validated against their authenticated reference cell lines. The ATCC Modification of DMEM uses precisely 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate — concentrations validated for ATCC-maintained cell lines including HeLa, MCF-7, A549, HEK-293, and Vero. Removing phenol red delivers an autofluorescence-free, hormone-interference–free baseline for fluorescence assays and hormone-sensitive cell lines. FluxMPS™ adds 0.04 µm filtration to enable use in OoC platforms.

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma purity unavailable from conventional 0.22 µm filtered media.

1. 1

   0.1 µm Pre-filtration I — Large Particulate Removal

   Removes large aggregates and contaminants; protects downstream 0.04 µm membranes and chip geometries from fouling.

2. 2

   0.04 µm Pre-filtration II — Mycoplasma Barrier

   Retains mycoplasma (0.1–0.3 µm) and fine particulates — absent from standard 0.22 µm filtration.

3. 3

   0.1 µm Sterile-filtration I — Second-pass Redundancy

   Second-pass sterility redundancy; no breakthrough from Stage 1.

4. 4

   0.04 µm Sterile-filtration II — Final Polish

   Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Specialized applications requiring ATCC Modification — Phenol Red–Free

FluxMPS™ DCP-DMEMA-R1X is designed for research protocols where atcc modification — phenol red–free conditions are essential — combined with MPS-grade filtration purity for organ-on-a-chip and microfluidic platform compatibility.

Automated Bioreactors & Robotics

Optional 0.01 µm (10 nm) ultra-filtered variant available for automated bioreactor perfusion and robotic liquid handlers.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
• Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion circuits
• Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

DMEM High Glucose — ATCC Modification — Phenol Red–Free: 32 ingredients verified per lot with CAS numbers for full raw-material traceability. ATCC Modification formulation: 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate. Phenol red is absent. These concentrations are validated for ATCC-maintained cell lines — do not substitute standard DMEM for ATCC-protocol experiments.

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

How DCP-DMEMA-R1X compares

FluxMPS™ DCP-DMEMA-R1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMA-R1X — DMEM High Glucose ATCC Modification — Phenol Red–Free.

Supporting literature

Key publications supporting the research applications of FluxMPS™ DCP-DMEMA-R1X.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216–4220. doi:10.1073/pnas.77.7.4216
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
6. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j