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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEMH-GPB1X
$44.00
DCP-DMEMH-GPB1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains 25mM HEPES Contains Calcium Contains Magnesium Without Glucose Without Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-DMEMH-GPB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered glucose-free DMEM + 25 mM HEPES formulation engineered for metabolic flux analysis, 13C isotope tracing, Warburg-effect research, and any organ-on-a-chip application where carbon source identity must be precisely controlled. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers a 0.04 µm final polish well below the 0.22 µm cut-off of conventional media. Formulation: [+] L-Glutamine, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium | [-] Glucose, [-] Sodium Pyruvate, [-] Sodium Bicarbonate.

  • Glucose-free — add D-glucose, 13C-glucose, galactose, fructose, or any carbon source at your defined concentration
  • 25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO2; supports open-top chips and metabolic sampling outside incubators
  • 0.04 µm final nano-filtration — sub-mycoplasma-retentive purity; < 0.05 EU/mL endotoxin release specification for clean metabolic baselines
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Contains phenol red (15.9 mg/L) as a pH indicator — a phenol red–free variant of this base is available on request for imaging and Seahorse XF–style applications
  • Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
  • Custom glucose concentrations, carbon sources, and nutrients available on request
Incomplete basal medium. This formulation contains no glucose and no sodium pyruvate. A carbon source, serum or defined supplements must be added before use. See the FAQ for supplementation guidance.
CAT. NO.
DCP-DMEMH-GPB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • GlucoseNot included — researcher-defined
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [-] Glucose, [-] Sodium Pyruvate, [-] Sodium Bicarbonate
  • AppearanceOrange-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310 - 350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

When carbon source control is the experiment

Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh at the concentration and identity you choose — while ultra-low particulate delivery supports chip integrity and a < 0.05 EU/mL endotoxin release specification helps avoid inflammatory artefacts in metabolic readouts.

science

Complete carbon source control

Add D-glucose, 13C-glucose, U-13C6-galactose, fructose, or no sugar. Combine with palmitate or other fatty acids. Define carbon source identity and concentration precisely for every experiment.

target

Total metabolic control

Reintroduce glucose (Warburg), galactose (force OXPHOS), or pyruvate at your own defined concentrations. L-Glutamine is retained as the sole nitrogen and anaplerotic carbon source, so TCA-cycle entry is under your control from the first well.

water_drop

HEPES: CO2-stable pH

25 mM HEPES (pKa 7.3 at 37°C) helps maintain pH outside CO2 incubators — useful for metabolic sampling and open-top chip platforms where gas equilibration is impractical.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and a < 0.05 EU/mL endotoxin release specification support metabolic baselines that are not confounded by particulate or endotoxin load.

visibility

Low background for imaging

Ultra-low particulate counts from 0.04 µm final filtration give a clean particulate baseline for confocal and biosensor work. This formulation contains phenol red (15.9 mg/L); a phenol red–free variant is available on request where reduced autofluorescence is required.

tune

Customization on demand

Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — important for glucose-free metabolic assays where particulate contamination and endotoxin can independently confound metabolic readouts.

  1. 1

    0.1 µm Prefiltration I — Large Particulate Removal

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge from early fouling.

  2. 2

    0.04 µm Final filtration I — Mycoplasma-Retentive Barrier

    Retains mycoplasma (0.2–0.3 µm) and fine particulates not retained by standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II — Second-Pass Redundancy

    A dedicated second prefilter protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter under ISO Class 5 aseptic fill & finish.

Why filtration purity matters for metabolic assays

Subvisible particulates and elevated endotoxin can independently upregulate glycolysis via TLR4/NF-κB signalling, confounding glucose uptake, lactate production, and OCR measurements. FluxMPS™ DCP-DMEMH-GPB1X is produced to a < 0.05 EU/mL endotoxin release specification and NMT 25/mL particulate (≥10 µm) specification, addressing both variables at the specification level.

0.04
µm Final pore size — sub-mycoplasma-retentive polishing
4
Serial filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.04 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEMH-GPB1X Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I mycoplasma-retentive barrier, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable glucose-free DMEM HEPES for organ-on-a-chip applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DMEMH-GPB1X
Applications

Metabolic research and OoC applications

FluxMPS™ DCP-DMEMH-GPB1X is purpose-built for experiments where carbon source identity must be controlled — from metabolic flux analysis and 13C isotope tracing to glucose dose-response studies on organ-on-a-chip platforms.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors and liquid-handling robotics running continuous perfusion, an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this base formulation is available, minimizing valve fouling and sensor drift over extended unattended runs.

  • Total Particulate Exclusion: 0.01 µm polish removes particulates below the resolution of standard 0.04 µm media
  • Valve & Sensor Protection: Reduces particulate-driven fouling in automated dosing valves and inline sensors
  • Extended Perfusion Stability: Supports longer unattended run times between system maintenance cycles

Inquiry Required: The 0.01 µm MPS Grade variant is available by request. Contact support@diagnocine.com.

Note on Seahorse XF assays: This formulation contains phenol red and is therefore not recommended for Agilent Seahorse XF real-time assays, which require phenol red-free, bicarbonate-free medium. A phenol red–free variant of this glucose-free, HEPES-buffered base is available on request — contact support@diagnocine.com.

Metabolomics

13C Metabolic Flux Analysis

Add U-13C6-glucose at any specific activity without background dilution. HEPES helps maintain pH during NMR sample preparation outside incubators.

13C tracingNMR metabolomicsLC-MS/MS
Cancer Biology

Warburg Effect & OXPHOS Switching

Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without media changes.

MCF-7MDA-MB-231HeLaA549
Microfluidics

Glucose Dose-Response on Chip

Perfuse defined glucose gradients through OoC channels to study dose-dependent metabolic responses in endothelial, beta-cell, or hepatocyte chips.

OoCToCDiabetes-on-chipLiver-on-chip
Stem Cell Biology

iPSC Metabolic Maturation

Glucose-free base enables galactose-forced OXPHOS maturation of iPSC-derived cardiomyocytes and hepatocytes, improving metabolic phenotype fidelity.

iPSC-CMiPSC-HepiPSC-β cells
Nutrient Biology

Nutrient Deprivation Studies

Starvation experiments, glucose withdrawal, and nutrient re-feeding protocols with defined timing and concentration control.

AutophagymTOR signalingAMPK activation
Live-Cell Imaging

Metabolic Imaging & Biosensors

This formulation contains phenol red; a phenol red–free variant is available on request for FLIM, NAD(P)H imaging, glucose FRET sensors, and TEER measurements requiring reduced autofluorescence.

FLIMGlucose sensorsTEERConfocal
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [-] Glucose, [-] Sodium Pyruvate, [-] Sodium Bicarbonate
Appearance Orange-colored, clear solution
Glucose Not included — add at desired concentration
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 310 - 350 mOsm/kg H2O
Total ingredients 31
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification; see Quality Assurance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)

Available pack sizes: 500 mL, 1000 mL.

Formulation

Full composition (mg/L)

Glucose-free DMEM + HEPES scaffold: 31 ingredients verified per lot with CAS numbers. Glucose is intentionally absent — add your carbon source of choice. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
Phenol red sodium salt 34487-61-1 15.900
HEPES 7365-45-9 5958.000
Carbon source supplementation: Glucose is not included. For standard culture add D-glucose (sterile-filtered stock) at 1000 mg/L (low) or 4500 mg/L (high). For 13C tracing add U-13C6-glucose. For OXPHOS forcing add D-galactose (2000 mg/L). Contact support@diagnocine.com for custom carbon source pre-formulations.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, controlled for trace metals and total organic carbon.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending between batches; Certificate of Analysis for every batch.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310 - 350 mOsm/kg H2O. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GPB1X compares

FluxMPS™ DCP-DMEMH-GPB1X vs. conventional glucose-free DMEM and standard DMEM for metabolic assays.

Parameter DCP-DMEMH-GPB1X (FluxMPS™) Conventional GF-DMEM
(0.22 µm filtered)		 Standard DMEM
(fixed glucose, 0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
Formulation [+] L-Glutamine, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [-] Glucose, [-] Sodium Pyruvate, [-] Sodium Bicarbonate Fixed formulation, no HEPES option Fixed glucose, fixed formulation
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration passes 4 (0.1 µm ×2 + 0.04 µm ×2) 1 (0.22 µm) 1 (0.22 µm)
Mycoplasma-retentive filtration check_circle Yes (0.04 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate compliance check_circle Yes cancel No cancel No
Water quality Ultrapure Type 1, 18.2 MΩ·cm Not specified Not specified
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Seahorse XF compatible cancel No (contains phenol red; phenol red–free variant available) check_circle Yes (phenol red–free) cancel Carbon source fixed
Microfluidic channel compatibility check_circle Yes cancel Limited cancel Limited
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-GPB1X glucose-free DMEM + 25 mM HEPES.

Yes. The quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) delivers a 0.04 µm final polish suited to microfluidic channel geometries, and the glucose-free, HEPES-buffered formulation gives precise control over carbon source identity for OoC metabolic studies, glucose dose-response gradients, and diabetes- or liver-on-chip models.
DCP-DMEMH-GPB1X is processed through two paired prefilter + final-filter stages: 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish). Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter. The 0.04 µm final pore size is well below the 0.22 µm cut-off of conventional media and is mycoplasma-retentive (mycoplasma range 0.2–0.3 µm).
Both carbon sources are removed so researchers can define the exact energy substrate and concentration — critical for comparing glycolysis (add D-glucose at 1000–4500 mg/L), oxidative phosphorylation (add D-galactose at 2000 mg/L), or fatty acid oxidation (add palmitate-BSA conjugate with no exogenous sugar). For 13C tracing, add U-13C6-glucose at the desired specific activity. L-Glutamine is retained as the sole nitrogen and anaplerotic carbon source. Contact support@diagnocine.com for stock preparation guidance.
No. This formulation is bicarbonate-free and CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation. This is useful for metabolic sampling, open-top chip platforms, and any protocol run outside a CO2 incubator.
Yes. Filter serum and other protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before adding to avoid stripping serum components; defined, protein-free additions such as glucose or galactose stocks may be filtered at 0.1 µm. Do not use 0.04 µm membranes for supplement filtration — they retain lipoproteins and heavily adsorb low-abundance growth factors.
FluxMPS™ DCP-DMEMH-GPB1X is produced to a release specification of < 0.05 EU/mL by LAL assay (USP <85>), controlled per manufacturing batch. Every batch is tested before release against this specification and against pH, osmolality, and sterility. A Certificate of Analysis is available on request.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting glucose-free DMEM in metabolic flux analysis, isotope tracing, and organ-on-a-chip metabolic studies.

  1. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  2. Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
  3. Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
  4. Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
  5. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  7. Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
  8. Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
  9. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x

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