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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-DMEMH-GPR1X
$71.50
DCP-DMEMH-GPR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Without Phenol Red Without Glucose Without Sodium Pyruvate

FluxMPS™ DCP-DMEMH-GPR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) glucose-free, sodium-pyruvate-free DMEM formulated with 25 mM HEPES, built for metabolic flux analysis, 13C isotope tracing, Warburg-effect research, and organ-on-a-chip applications where carbon source identity must be precisely controlled. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium | [-] Phenol Red, [-] Glucose, [-] Sodium Pyruvate.

  • Glucose-free and sodium-pyruvate-free — add D-glucose, U-13C6-glucose, galactose, fructose, or another carbon source at the exact concentration your protocol requires
  • 25 mM HEPES (pKa 7.3 at 37°C) provides supplemental buffering alongside the formulation's sodium bicarbonate — see the CO2 note in Technical Specifications for the recommended incubator setting
  • Quadruple-stage filtration: 0.1 µm prefilter I → 0.04 µm final filter I → 0.1 µm prefilter II → 0.04 µm final filter II (polish)
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP 85), tested per manufacturing batch, not per unit
  • Phenol red not added to this formulation — reduces optical background for imaging-based assays
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium | [-] Phenol Red, [-] Glucose, [-] Sodium Pyruvate
  • Custom glucose concentrations, carbon sources, and nutrient modifications available on request — support@diagnocine.com
Incomplete basal medium. This formulation contains no glucose and no sodium pyruvate. A carbon source, serum, or defined supplements must be added before use. See the FAQ for supplementation guidance.
CAT. NO.
DCP-DMEMH-GPR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Phenol Red: 1X Liquid
  • GlucoseNot included — researcher-defined
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium | [-] Phenol Red, [-] Glucose, [-] Sodium Pyruvate
  • AppearanceOrange-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO

Available pack sizes: 500 mL, 1000 mL

Why FluxMPS™

When carbon source control is the experiment

Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free, pyruvate-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh, at the identity and concentration you choose — while ultra-low particulate delivery supports chip integrity and a < 0.05 EU/mL endotoxin release specification reduces the risk of inflammatory confounds in metabolic readouts.

science

Complete carbon source control

Add D-glucose, U-13C6-glucose, galactose, fructose, or no sugar. Combine with palmitate or other fatty acids. Define carbon source identity and concentration precisely for every experiment.

bolt

Dual buffering, understood on its own terms

This formulation carries both sodium bicarbonate and 25 mM HEPES. The bicarbonate concentration determines the recommended CO2 environment (see Technical Specifications); HEPES adds buffering capacity that helps limit pH drift during brief periods outside CO2 control, such as bench-top handling or short sampling steps.

water_drop

Ultrapure-grade water

Formulated with Type 1 water (18.2 MΩ·cm), supporting a low trace-metal and organic-carbon background for sensitive metabolic assays.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and a < 0.05 EU/mL endotoxin release specification help keep metabolic baselines free of particulate and endotoxin confounds.

visibility

Low background for imaging

This formulation does not include phenol red, avoiding phenol red's inherent optical absorbance. Combined with a low particulate baseline (0.04 µm final filtration), it supports confocal microscopy, TEER measurement, and biosensor OoC platforms.

tune

Customization on demand

Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two prefilter-plus-final-filter pairs, reaching a final 0.04 µm polish. This matters for glucose-free metabolic assays, where particulate contamination and endotoxin can independently confound glycolytic and mitochondrial readouts.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and aggregates; protects the first 0.04 µm final filter from early fouling.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2's effluent.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Why filtration purity matters for metabolic assays

Subvisible particulates and elevated endotoxin can independently upregulate glycolytic signalling, confounding glucose uptake, lactate production, and oxygen consumption measurements. FluxMPS™ DCP-DMEMH-GPR1X targets both variables: a < 0.05 EU/mL endotoxin release specification and a validated 0.04 µm final filtration stage.

5×
Fewer particulates than 0.22 µm media (by count)
0.04
µm final pore size across two filter pairs
Sterility: No growth after 14-day incubation (USP <71>). Mycoplasma: controlled by 0.1 µm/0.04 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMH-GPR1X Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate, Phenol Red: 1X Liquid, Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II polish, Microfluidics Suitable glucose-free HEPES DMEM for organ-on-a-chip applications, Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DMEMH-GPR1X
Applications

Metabolic research and OoC applications

FluxMPS™ DCP-DMEMH-GPR1X is built for experiments where carbon source identity must be controlled — from Warburg-effect studies to 13C metabolic flux analysis and glucose dose-response studies on organ-on-a-chip platforms. Note: because this formulation contains sodium bicarbonate, it is not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium; this product is phenol red-free but not bicarbonate-free. A bicarbonate-free custom formulation can be requested for Seahorse XF work.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For continuous perfusion systems and robotic liquid handling, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered variant of this formulation — the separate MPS Grade tier described in the Grade note above — intended to further reduce particulate load in automated, valve-dense fluidic paths.

  • Total Particulate Exclusion: extended nano-filtration for long-duration perfusion runs
  • Valve & Sensor Protection: reduced particulate load in microvalves and inline sensors
  • Extended Perfusion Stability: supports longer intervals between filter changes in closed-loop systems

Inquiry Required: the 0.01 µm MPS Grade variant is available by request. Contact support@diagnocine.com.

Metabolomics

13C Metabolic Flux Analysis

Add U-13C6-glucose at any specific activity without background dilution from endogenous glucose. HEPES helps maintain pH during sample preparation outside the incubator.

13C tracingNMR metabolomicsLC-MS/MS
Cancer Biology

Warburg Effect & OXPHOS Switching

Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without a media change.

MCF-7MDA-MB-231HeLaA549
Microfluidics

Glucose Dose-Response on Chip

Perfuse defined glucose gradients through OoC channels to study dose-dependent metabolic responses in endothelial, beta-cell, or hepatocyte chips.

OoCToCDiabetes-on-chipLiver-on-chip
Stem Cell Biology

iPSC Metabolic Maturation

Glucose-free base enables galactose-forced OXPHOS maturation protocols for iPSC-derived cardiomyocytes and hepatocytes.

iPSC-CMiPSC-HepiPSC-beta cells
Nutrient Biology

Nutrient Deprivation Studies

Starvation experiments, glucose withdrawal, and nutrient re-feeding protocols with defined timing and concentration control.

AutophagymTOR signalingAMPK activation
Live-Cell Imaging

Metabolic Imaging & Biosensors

Absence of phenol red and a low particulate baseline support confocal imaging, NAD(P)H imaging, glucose biosensors, and TEER measurement on metabolic chips.

ConfocalGlucose sensorsTEER
Technical Specifications

Analytical release specifications

Every lot is released against the full specification matrix below. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium | [-] Phenol Red, [-] Glucose, [-] Sodium Pyruvate
Appearance Orange-colored, clear solution
Glucose Not included — add at desired concentration
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Total ingredients 31 components across 4 formulation categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm/0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement ~10% CO2 recommended to balance 3700 mg/L sodium bicarbonate at pH 7.4; the 25 mM HEPES buffer adds stability during brief periods outside CO2 control
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glucose-free, pyruvate-free DMEM + HEPES scaffold: 31 ingredients across 4 formulation categories, verified per lot with CAS numbers. Glucose is intentionally absent — add your carbon source of choice. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
i-Inositol 87-89-8 7.200
OTHERS
HEPES 7365-45-9 5958.000
Carbon source supplementation: Glucose is not included. For standard culture add D-glucose (sterile-filtered stock) at 1000 mg/L (low) or 4500 mg/L (high). For 13C tracing add U-13C6-glucose. For OXPHOS forcing add D-galactose (2000 mg/L). Contact support@diagnocine.com for custom carbon source pre-formulations.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, supporting low trace-metal and organic-carbon background for metabolic flux measurements.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending between batches; a Certificate of Analysis is issued for every lot.

Endotoxin — USP <85> BET

LAL assay, assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310–350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GPR1X compares

FluxMPS™ DCP-DMEMH-GPR1X versus conventional glucose-free DMEM and standard DMEM for metabolic assays.

Parameter DCP-DMEMH-GPR1X (FluxMPS™) Conventional GF-DMEM (0.22 µm filtered) Standard DMEM (fixed glucose, 0.22 µm)
Grade Microfluidics Suitable Conventional (not microfluidics rated) Conventional (not microfluidics rated)
Glucose-free, pyruvate-free, phenol red-free DMEM + HEPES check_circle Yes cancel No cancel No
Glucose content None — researcher-defined None Fixed (1–4.5 g/L)
HEPES (25 mM) check_circle Yes Usually no No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (two 0.1/0.04 µm pairs) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate compliance check_circle Yes cancel Not specified cancel Not specified
Water quality Type 1, 18.2 MΩ·cm Not specified Not specified
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes cancel Not validated cancel Not validated
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-GPR1X glucose-free DMEM + 25 mM HEPES.

Yes. The quadruple-stage filtration to a 0.04 µm final cut-off, low endotoxin release specification (< 0.05 EU/mL), and controlled, researcher-defined carbon source make this formulation suitable for organ-on-a-chip, tissue-on-a-chip, and other microphysiological system (MPS) platforms where microchannel fouling and metabolic background must be minimized. It is Microfluidics Suitable; it is not the separate 0.01 µm MPS Grade tier (see the Grade note in the Purity Architecture section).
The medium passes through two paired prefilter-plus-final-filter stages (0.1 µm then 0.04 µm, twice), reaching a 0.04 µm final pore size versus the 0.22 µm typical of conventional media. This delivers approximately 5× lower particulate counts by count and adds a mycoplasma-retentive filtration stage not present in single-pass 0.22 µm processing.
Both carbon sources are omitted so that carbon source identity and concentration are entirely under your control. Add D-glucose as a sterile-filtered stock at 1000 mg/L (low-glucose) or 4500 mg/L (high-glucose). For 13C tracing, add U-13C6-glucose at the same concentration. For OXPHOS forcing, replace glucose with D-galactose (2000 mg/L). Sodium pyruvate can be added separately if your protocol requires it. Contact support@diagnocine.com for stock preparation guidance.
This formulation contains 3700 mg/L sodium bicarbonate, which requires approximately 10% CO2 to maintain pH 7.4 (Henderson-Hasselbalch). The added 25 mM HEPES provides supplemental buffering that helps limit pH drift during brief periods outside CO2 control, such as bench-top handling, but does not remove the need for a CO2-controlled incubator for routine culture. Validate the exact CO2 setting for your incubator and vessel geometry.
Yes. Serum and other protein-containing additions should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — not 0.04 µm, which would retain much of the protein and lipoprotein fraction. Defined, protein-free additions such as small-molecule carbon sources can be filtered at 0.1 µm or 0.2 µm.
The release specification for DCP-DMEMH-GPR1X is < 0.05 EU/mL, assessed by LAL assay (USP <85>, Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification before product ships. A Certificate of Analysis is available on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting glucose-free DMEM in metabolic flux analysis, isotope tracing, and organ-on-a-chip metabolic studies.

  1. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  2. Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
  3. Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
  4. Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
  5. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  7. Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
  8. Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
  9. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x

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