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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-DMEMH-GQPBR1X
$71.50
DCP-DMEMH-GQPBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains HEPES (25 mM) Contains Calcium Contains Magnesium Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red Without Glucose Without Sodium Pyruvate

FluxMPS™ DCP-DMEMH-GQPBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) glucose-free DMEM + 25 mM HEPES formulation engineered for metabolic flux analysis, 13C isotope tracing, Warburg-effect research, and organ-on-a-chip (OoC) applications where carbon source identity must be precisely controlled. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Glucose-free — add D-glucose, 13C-glucose, galactose, fructose, or any carbon source at your defined concentration
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO2-independent pH stability; suited to Seahorse XF, open-top chips, and metabolic sampling outside incubators
  • Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size for microchannel-safe particulate control
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) for clean metabolic baselines
  • Formulation: [+] HEPES, Calcium, Magnesium | [-] Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom glucose concentrations, carbon sources, and nutrient modifications available on request
Incomplete basal medium. This formulation contains no glucose and no sodium pyruvate. A carbon source, serum or defined supplements must be added before use. See the FAQ for supplementation guidance.
CAT. NO.
DCP-DMEMH-GQPBR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • GlucoseNot included — researcher-defined
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] HEPES, Calcium, Magnesium | [-] Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red
  • AppearancePale yellow colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

When carbon source control is the experiment

Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh at the concentration and identity you choose, while a 0.04 µm final filter and a < 0.05 EU/mL endotoxin release specification reduce the risk of inflammatory artefacts confounding metabolic readouts.

science

Complete carbon source control

Add D-glucose, 13C-glucose, U-13C6-galactose, fructose, or no sugar. Combine with palmitate or other fatty acids. Define carbon source identity and concentration precisely for every experiment.

analytics

Seahorse XF & metabolomics ready

A glucose-free, bicarbonate-free, phenol red–free base is the standard starting point for Seahorse XF Glycolysis Stress Tests. HEPES maintains pH during real-time injection protocols without CO2 dependency.

water_drop

HEPES: CO2-stable pH

25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.2–7.4 outside CO2 incubators — useful for metabolic sampling, Seahorse injection events, and open-top chip platforms.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and a < 0.05 EU/mL endotoxin release specification support clean metabolic baselines in microfluidic channels.

visibility

Low background for imaging

This formulation removes phenol red, eliminating pH-indicator dye background and associated estrogen-receptor agonist activity. Combined with 0.04 µm particulate control, it supports a clean particulate baseline for live-cell imaging, TEER, and biosensor OoC platforms. Note: this medium retains riboflavin, which contributes inherent flavin autofluorescence common to DMEM-based media.

tune

Customization on demand

Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two prefilter-plus-final-filter pairs, reach a final 0.04 µm polish. This is particularly relevant for glucose-free metabolic assays, where particulate contamination and endotoxin can independently confound innate immune and metabolic signalling.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-range organisms (0.2–0.3 µm) that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge in the train.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish under ISO Class 5 (Class 100) conditions.

Why filtration purity matters for metabolic assays

Subvisible particulates and elevated endotoxin can independently upregulate glycolysis via TLR4/NF-κB signalling, which would confound glucose uptake, lactate production, and oxygen consumption rate (OCR) measurements. FluxMPS™ DCP-DMEMH-GQPBR1X keeps both variables within specification: < 0.05 EU/mL endotoxin and NMT 25 particles/mL (≥10 µm).

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.04 µm barrier filtration (not tested per lot); this is a filtration control, not a per-lot mycoplasma assay result.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMH-GQPBR1X Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - glucose-free Microfluidics Suitable DMEM HEPES for organ-on-a-chip applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DMEMH-GQPBR1X
Applications

Metabolic research and OoC applications

FluxMPS™ DCP-DMEMH-GQPBR1X is built for experiments where carbon source identity must be controlled — from Seahorse XF assays to 13C metabolic flux analysis and glucose dose-response studies on organ-on-a-chip platforms.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For closed-loop, automated microfluidic bioreactors and robotic liquid-handling platforms, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, engineered to further reduce valve fouling and sensor drift over extended perfusion runs.

  • Total Particulate Exclusion: 0.01 µm final cut-off for the most demanding closed microfluidic loops
  • Valve & Sensor Protection: minimizes particulate accumulation on inline sensors and microvalves
  • Extended Perfusion Stability: supports longer unattended run times in automated bioreactor systems

Inquiry Required: The 0.01 µm MPS Grade variant is available on request. Contact support@diagnocine.com.

Metabolomics

13C Metabolic Flux Analysis

Add U-13C6-glucose at any specific activity without background dilution. HEPES maintains pH during NMR sample preparation outside incubators.

13C tracingSeahorse XFNMR metabolomicsLC-MS/MS
Cancer Biology

Warburg Effect & OXPHOS Switching

Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without media changes.

MCF-7MDA-MB-231HeLaA549
Microfluidics

Glucose Dose-Response on Chip

Perfuse defined glucose gradients through OoC channels to study dose-dependent metabolic responses in endothelial, beta-cell, or hepatocyte chips.

OoCToCDiabetes-on-chipLiver-on-chip
Stem Cell Biology

iPSC Metabolic Maturation

Glucose-free base enables galactose-forced OXPHOS maturation of iPSC-derived cardiomyocytes and hepatocytes, supporting more mature metabolic phenotypes.

iPSC-CMiPSC-HepiPSC-β cells
Nutrient Biology

Nutrient Deprivation Studies

Starvation experiments, glucose withdrawal, and nutrient re-feeding protocols with defined timing and concentration control.

AutophagymTOR signalingAMPK activation
Live-Cell Imaging

Metabolic Imaging & Biosensors

Phenol red–free formulation removes pH-indicator background fluorescence. Combined with 0.04 µm particulate filtration, it supports FLIM, NAD(P)H imaging, glucose FRET sensors, and TEER measurements on metabolic chips.

FLIMGlucose sensorsTEERConfocal
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Available pack sizes: 500 mL, 1000 mL. CoA available at support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] HEPES, Calcium, Magnesium | [-] Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red
Appearance Pale yellow colored, clear solution
Glucose Not included — add at desired concentration
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Total ingredients 29
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) maintains pH 7.2–7.4 at 37°C without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glucose-free DMEM + HEPES scaffold: 29 ingredients verified per lot with CAS numbers, across 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others). Glucose is intentionally absent — add your carbon source of choice. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
HEPES 7365-45-9 5958.000
i-Inositol 87-89-8 7.200
Carbon source supplementation: Glucose is not included. For standard culture, add D-glucose (sterile-filtered stock) at 1000 mg/L (low) or 4500 mg/L (high). For 13C tracing add U-13C6-glucose. For OXPHOS forcing add D-galactose (2000 mg/L). Contact support@diagnocine.com for custom carbon source pre-formulations.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water, controlled for trace metals and organic carbon.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending between batches; a Certificate of Analysis is available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310–350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GQPBR1X compares

FluxMPS™ DCP-DMEMH-GQPBR1X vs. conventional glucose-free DMEM and standard DMEM for metabolic assays.

Parameter DCP-DMEMH-GQPBR1X (FluxMPS™) Conventional GF-DMEM
(0.22 µm filtered)		 Standard DMEM
(fixed glucose, 0.22 µm)
Grade Microfluidics Suitable Standard grade Standard grade
Glucose-free DMEM + HEPES base — CO2-independent, all carbon/nitrogen sources and phenol red removed; researcher defines everything check_circle Yes cancel No cancel No
Glucose content None — researcher-defined None Fixed (1–4.5 g/L)
HEPES (25 mM) check_circle Yes cancel Usually no cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (0.1 µm ×2 + 0.04 µm ×2) 1 1
Endotoxin specification (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Mycoplasma-retentive filtration check_circle Yes (0.04 µm) cancel No cancel No
Seahorse XF compatible check_circle Yes (GF base + HEPES) check_circle Yes (no HEPES) cancel Carbon source fixed
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-GQPBR1X glucose-free DMEM + 25 mM HEPES.

Yes. The 0.04 µm quadruple-stage filtration reduces particulate load relevant to microchannel geometries, the < 0.05 EU/mL endotoxin release specification helps avoid inflammatory confounds, and the glucose-free, HEPES-buffered, phenol red–free formulation gives researchers full control of carbon source and optical background — all useful properties for OoC and microphysiological system (MPS) work.
DCP-DMEMH-GQPBR1X passes through four filtration stages run as two prefilter-plus-final-filter pairs: 0.1 µm prefiltration, 0.04 µm final filtration, a second 0.1 µm prefilter, and a second 0.04 µm final polish. Standard media is typically filtered once, at 0.22 µm. The 0.04 µm final pore size is well below the 0.22 µm used for routine cell culture media filtration, giving a lower particulate count by comparison.
All five variable components are removed so that carbon source, nitrogen source, buffering system, and optical background can each be defined by the researcher rather than fixed by the manufacturer. To supplement: add D-glucose as a sterile-filtered stock (e.g. 500 g/L in water, 0.2 µm filtered) at 1000 mg/L (low-glucose) or 4500 mg/L (high-glucose). For 13C tracing, add U-13C6-glucose at the same concentration. For OXPHOS forcing, replace glucose with D-galactose (2000 mg/L). For fatty acid oxidation studies, add no sugar and supplement with a palmitate-BSA conjugate. Contact support@diagnocine.com for stock preparation guidance.
No. This formulation is CO2-independent — 25 mM HEPES (pKa 7.3 at 37°C) alone maintains pH 7.2–7.4 without gas supplementation. This is particularly useful for Seahorse XF assays, where CO2 fluctuation during the measurement period would otherwise shift pH and confound OCR/ECAR readings, and for open-top chip platforms and metabolic sampling performed outside an incubator.
Yes. When adding serum, growth factors, or other protein-containing supplements, pre-filter the supplement stock through a 0.2 µm low-protein-binding PES or PVDF membrane before combining it with the base medium. Do not use a 0.04 µm membrane for serum or protein-containing additions: a 0.04 µm pore size retains immunoglobulins, lipoproteins, and much of the lipid fraction that makes serum functionally active, and will clog quickly. For defined, protein-free additions such as small-molecule carbon sources, a 0.1 µm or 0.2 µm filter is appropriate.
The release specification is < 0.05 EU/mL, verified by LAL assay per USP <85> on every manufacturing batch before release (assay sensitivity 0.005 EU/mL). Endotoxin is controlled per batch, not per individual unit. This matters for metabolic research: endotoxin can activate TLR4/NF-κB signalling, which independently affects glycolytic activity and could confound glucose uptake, lactate production, and ECAR measurements.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting glucose-free DMEM in metabolic flux analysis, Seahorse XF assays, isotope tracing, and organ-on-a-chip metabolic studies.

  1. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  2. Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
  3. Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
  4. Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
  5. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  7. Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
  8. Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
  9. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x

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