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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPEs w/o Glucose, L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-DMEMH-GQBR1X
$71.50
DCP-DMEMH-GQBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Sodium Pyruvate Without Glucose Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-DMEMH-GQBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered glucose-free DMEM + 25 mM HEPES formulation engineered for metabolic flux analysis, 13C isotope tracing, Warburg-effect research, and organ-on-a-chip applications where carbon source identity must be precisely controlled. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Glucose, [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red.

  • Glucose-free — add D-glucose, 13C-glucose, galactose, fructose, or any carbon source at your defined concentration
  • 25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO₂; suited to Seahorse XF, open-top chips, and metabolic sampling outside incubators
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off — mycoplasma-retentive filtration (not tested per lot)
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) for clean metabolic baselines
  • Formulation: [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Glucose, [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
  • Manufactured under an ISO 13485:2016 quality management system; Ultrapure Type 1 water (18.2 MΩ·cm)
  • Custom glucose concentrations, carbon sources, and nutrients available on request
CAT. NO.
DCP-DMEMH-GQBR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • GlucoseNot included — researcher-defined
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate, [-] Glucose, [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
  • AppearancePale yellow colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

When carbon source control is the experiment

Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh at the concentration and identity you choose — while ultra-low particulate delivery supports chip integrity and a < 0.05 EU/mL endotoxin release specification limits inflammatory confounds in metabolic readouts.

science

Complete carbon source control

Add D-glucose, 13C-glucose, U-13C6-galactose, fructose, or no sugar. Combine with palmitate or other fatty acids. Define carbon source identity and concentration precisely for every experiment.

analytics

Seahorse XF & metabolomics ready

Glucose-free base is the standard for Seahorse XF Glycolysis Stress Tests. HEPES maintains pH during real-time injection protocols without CO₂ dependency — no pH drift between ports.

water_drop

HEPES: CO₂-stable pH

25 mM HEPES (pKa 7.3 at 37°C) supports pH stability outside CO₂ incubators — useful for metabolic sampling, Seahorse injection events, and open-top chip platforms.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and a < 0.05 EU/mL endotoxin release specification support metabolic baselines that are not confounded by particulate load or LPS-driven glycolytic signalling.

visibility

Imaging-compatible formulation

Phenol red–free formulation removes phenol red autofluorescence and estrogen-receptor agonist activity for live-cell imaging, TEER, and biosensor OoC platforms. Riboflavin (0.400 mg/L) contributes intrinsic fluorescence near 525 nm — validate for ultra-sensitive fluorescence assays.

tune

Customization on demand

Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish. Particulate contamination and endotoxin can each independently activate innate-immune metabolic reprogramming, which is of particular concern for glucose-free metabolic assays where baseline signalling must stay clean.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and particulate; protects the first 0.04 µm cartridge and downstream chip geometries from early fouling.

  2. 2

    0.04 µm Final filtration I — Mycoplasma-retentive

    Retains mycoplasma (0.2–0.3 µm) and fine particulates not retained by standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge — full redundancy for the final-filtration pair.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish.

Why filtration purity matters for metabolic assays

Subvisible particulates and endotoxin have each been linked to TLR4/NF-κB signalling that can upregulate glycolysis — a potential confound for glucose uptake, lactate production, and OCR measurements. FluxMPS™ DCP-DMEMH-GQBR1X is filtered and released to control both variables: a < 0.05 EU/mL endotoxin specification and NMT 25 particles/mL (≥10 µm).

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma control is by 0.1 µm and 0.04 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMH-GQBR1X Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid, Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I mycoplasma-retentive, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish, Microfluidics Suitable glucose-free HEPES DMEM for organ-on-a-chip applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DMEMH-GQBR1X
Applications

Metabolic research and OoC applications

FluxMPS™ DCP-DMEMH-GQBR1X is purpose-built for experiments where carbon source identity must be controlled — from classic Seahorse XF assays to advanced 13C metabolic flux analysis and glucose dose-response studies on organ-on-a-chip platforms. For automated bioreactor and robotic handling systems requiring particulate exclusion beyond 0.04 µm, an optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available on request.

Seahorse XF & Real-Time Metabolic Flux

Gold Standard Metabolic Assay Base

Glucose-free DMEM is the manufacturer-recommended base for Seahorse XF Glycolysis Stress Tests (add glucose, oligomycin, 2-DG) and Mito Stress Tests (add glucose + glutamine + pyruvate, then oligomycin, FCCP, rotenone/antimycin). The 25 mM HEPES in DCP-DMEMH-GQBR1X limits pH drift between injection events without requiring CO₂ re-equilibration.

  • Glycolysis Stress Test: Glucose-free base supports a low background ECAR reading before glucose injection
  • Mito Stress Test: Add glucose + glutamine + pyruvate to defined concentrations — no contribution from a pre-existing carbon source
  • 13C Isotope Tracing: Add U-13C6-glucose at exact specific activity without dilution from background glucose

Inquiry Required: Custom glucose, galactose, or 13C-carbon source co-formulations available. Contact support@diagnocine.com.

Metabolomics

13C Metabolic Flux Analysis

Add U-13C6-glucose at any specific activity without background dilution. HEPES maintains pH during NMR sample preparation outside incubators.

13C tracingSeahorse XFNMR metabolomicsLC-MS/MS
Cancer Biology

Warburg Effect & OXPHOS Switching

Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without media changes.

MCF-7MDA-MB-231HeLaA549
Microfluidics

Glucose Dose-Response on Chip

Perfuse defined glucose gradients through OoC channels to study dose-dependent metabolic responses in endothelial, beta-cell, or hepatocyte chips.

OoCToCDiabetes-on-chipLiver-on-chip
Stem Cell Biology

iPSC Metabolic Maturation

Glucose-free base enables galactose-forced OXPHOS maturation protocols for iPSC-derived cardiomyocytes and hepatocytes.

iPSC-CMiPSC-HepiPSC-β cells
Nutrient Biology

Nutrient Deprivation Studies

Starvation experiments, glucose withdrawal, and nutrient re-feeding protocols with defined timing and concentration control.

AutophagymTOR signalingAMPK activation
Live-Cell Imaging

Metabolic Imaging & Biosensors

Phenol red–free formulation removes phenol red background for FLIM, NAD(P)H imaging, glucose FRET sensors, and TEER measurements on metabolic chips. Riboflavin (0.400 mg/L) contributes intrinsic fluorescence near 525 nm — validate for ultra-sensitive fluorescence assays.

FLIMGlucose sensorsTEERConfocal
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Glucose, [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow colored, clear solution
Glucose Not included — add at desired concentration
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Total ingredients 30 components across 4 category groups (3 tabs)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1/0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glucose-free DMEM + HEPES scaffold: 30 ingredients verified per lot with CAS numbers. Glucose is intentionally absent — add your carbon source of choice. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) and sodium pyruvate are listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
Sodium pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Carbon source supplementation: Glucose is not included. For standard culture add D-glucose (sterile-filtered stock) at 1000 mg/L (low) or 4500 mg/L (high). For 13C tracing add U-13C6-glucose. For OXPHOS forcing add D-galactose (2000 mg/L). Contact support@diagnocine.com for custom carbon source pre-formulations.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, trace-metal and organic-carbon (TOC) controlled — minimizes background contaminants relevant to sensitive metabolic flux measurements.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending between batches, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL, assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310–350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GQBR1X compares

FluxMPS™ DCP-DMEMH-GQBR1X vs. conventional glucose-free DMEM and standard DMEM for metabolic assays.

Parameter DCP-DMEMH-GQBR1X (FluxMPS™) Conventional GF-DMEM
(0.22 µm filtered)		 Standard DMEM
(fixed glucose, 0.22 µm)
Grade Microfluidics Suitable Not designated Not designated
Glucose-free + Glutamine-free DMEM + HEPES — CO₂-independent, no phenol red; pyruvate included as defined carbon source for open-top fluorescence chips check_circle Yes cancel No cancel No
Glucose content None — researcher-defined None Fixed (1–4.5 g/L)
HEPES (25 mM) check_circle Yes cancel Usually no cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (0.1 µm ×2 + 0.04 µm ×2) 1 (0.22 µm) 1 (0.22 µm)
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
Mycoplasma barrier filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
USP <788> particulate tested check_circle Yes cancel No cancel No
Seahorse XF compatible check_circle Yes (GF base + HEPES) check_circle Yes (no HEPES) cancel Carbon source fixed
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-GQBR1X glucose-free DMEM + 25 mM HEPES.

Yes. The glucose-free, HEPES-buffered, bicarbonate-free formulation is well suited to open-top microfluidic and organ-on-a-chip (OoC) platforms where CO₂ control is impractical and precise carbon-source dosing is required. The 0.04 µm final filtration supports microchannel-safe delivery.
DCP-DMEMH-GQBR1X is processed through a quadruple-stage train — two 0.1 µm prefilters, each paired with its own dedicated 0.04 µm final filter (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) — reaching a 0.04 µm final cut-off versus the single 0.22 µm pass used by conventional media. This retains mycoplasma-sized particles (0.2–0.3 µm) and supports approximately 5× lower particulate counts by count.
Glucose and L-glutamine are removed so researchers can add carbon and nitrogen sources at defined concentrations; sodium bicarbonate is removed for CO₂-independent HEPES-only buffering; phenol red is removed for imaging applications sensitive to background fluorescence and estrogen-receptor agonist activity. Sodium pyruvate (110 mg/L) is retained as a defined carbon source. To supplement: add D-glucose as a sterile-filtered stock at 1000 mg/L (low) or 4500 mg/L (high); for isotope tracing add U-13C6-glucose at the same concentration; for OXPHOS forcing replace glucose with D-galactose (2000 mg/L). Contact support@diagnocine.com for stock preparation guidance.
No. This formulation is CO₂-independent — 25 mM HEPES alone maintains pH 7.2–7.4 at 37°C without gas supplementation, since sodium bicarbonate has been removed from the formulation. HEPES buffering is particularly valuable for Seahorse XF assays, where CO₂ fluctuation during the 60–90 minute measurement period would otherwise shift pH and confound OCR/ECAR readings.
Yes. Serum and other protein-containing supplements should be filtered with a 0.2 µm low-protein-binding PES or PVDF membrane before addition — a 0.04 µm membrane is not appropriate for serum, as it retains IgM, lipoproteins, and other components that make serum functional. Defined, protein-free additions (e.g., glucose stock solutions) may be filtered at 0.1 µm or 0.2 µm.
Endotoxin is controlled per manufacturing batch and released to a specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Every batch is tested before release and must meet this specification along with sterility, pH, osmolality, conductivity, and appearance criteria. A Certificate of Analysis reporting the batch result is available on request.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting glucose-free DMEM in metabolic flux analysis, Seahorse XF assays, isotope tracing, and organ-on-a-chip metabolic studies.

  1. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  2. Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
  3. Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
  4. Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
  5. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  7. Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
  8. Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
  9. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x

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