0
Precision Quality to Your Research verified ISO 13485 Certified | SBA WOSB | NJ MWBE · NYC MWBE · Illinois BEP arrow_forward View Details

Denaturing Gel-Loading Buffer without Dye [4X]

Product#: DCP-DGLBWOD4X
$88.00
Availability:
Ships in 24 hours

FluxMPS™ Buffer
ISO 13485 Certified Manufacturing

FluxMPS™ Denaturing Gel-Loading Buffer without Dye [4X]

An MPS-grade, formamide- and EDTA-based denaturing gel-loading buffer supplied as a 4X concentrate for ssDNA, DNA, and RNA electrophoresis. Prepared from DEPC-treated Ultrapure Type 1 water processed through DiagnoCine's quadruple-stage filtration architecture, then terminally sterile-filtered, this dye-free formulation is engineered for microchannel-safe, low-background molecular biology workflows.

  • Ultrapure Type 1 water base processed through DiagnoCine's quadruple-stage filtration architecture (0.1 µm membrane twice and 0.04 µm membrane twice), helping prevent mycoplasma contamination
  • Finished buffer terminally sterile-filtered through a 0.22 µm membrane once
  • Formamide-based formulation denatures secondary structure in ssDNA, DNA, and RNA for denaturing agarose or PAGE electrophoresis
  • Contains EDTA to chelate divalent metal ions (Ca2+, Mg2+) and inhibit metal-dependent nuclease activity
  • Dye-free formulation — contains no SDS, bromophenol blue, or xylene cyanol
  • No detectable DNase or RNase activity after 18 hr incubation at room temperature
  • Supplied as 5 x 1 mL (4X), supporting 500 loadings (10 µl buffer + 30 µl sample = 40 µl/well)
  • Custom concentrations, pH, and additive formulations available on request
SKU: DCP-DGLBWOD4X UNSPSC: 41105335 Loading Buffer
Denaturing Gel-Loading Buffer without Dye, 4X Concentrate
  • Concentration4X
  • AppearanceClear, colorless liquid
  • Dye ContentNone (dye-free)
  • Key DenaturantFormamide
  • Chelating AgentEDTA
  • SterilityFiltered 0.22 µm membrane once
  • DNase ActivityNone detected (18 hr, RT)
  • RNase ActivityNone detected (18 hr, RT)
  • Storage-20°C
  • Shelf Life3 years
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard loading buffers fall short

Conventional 0.22 µm-filtered loading buffers, unpurified water sources, and dye-laden formulations can introduce subvisible particulate, nuclease contamination, and optical background into sensitive nucleic acid workflows. FluxMPS™ addresses each failure mode directly.

filter_alt

Microchannel-safe purity

Ultrapure Type 1 water base processed through a quadruple-stage filtration architecture (0.1 µm x2, 0.04 µm x2) minimizes particulate carried into downstream gel or chip-based workflows.

science

Verified nuclease-free

No detectable DNase or RNase activity after 18 hr incubation with plasmid DNA and ribosomal RNA at room temperature, protecting sample integrity during denaturation and loading.

water_drop

Ultrapure-grade water

DEPC-treated Ultrapure Type 1 water forms the buffer base, reducing background nuclease activity before it can affect ssDNA, DNA, or RNA samples.

visibility

Dye-free, low-background formulation

Free of SDS, bromophenol blue, and xylene cyanol, leaving downstream detection, blotting, or imaging steps unobstructed by tracking dye chemistry.

target

Defined denaturing chemistry

Formamide denatures secondary structure in ssDNA, DNA, and RNA, while EDTA chelates Ca2+ and Mg2+ to inhibit metal-dependent nucleases during handling.

tune

Customization on demand

Alternate concentrations, additional chemicals, compounds, proteins, supplements, or pH adjustments are available on request.

Purity Architecture

Quadruple-stage filtration system

The DEPC-treated Ultrapure Type 1 water used to prepare this buffer is processed through DiagnoCine's quadruple-stage filtration architecture — filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice — before compounding, helping prevent mycoplasma contamination during manufacturing.

  1. 1

    0.1 µm Pre-filtration I

    Removes large particulate and aggregates from the Ultrapure Type 1 water base, extending downstream filter life.

  2. 2

    0.04 µm Pre-filtration II

    Retains fine particulates and bioburden prior to buffer compounding.

  3. 3

    0.1 µm Sterile-filtration I

    Second-pass redundancy through a 0.1 µm membrane.

  4. 4

    0.04 µm Sterile-filtration II — Final Polish

    Ultimate polish of the water base prior to compounding, supporting a controlled aseptic fill.

Performance vs. conventional loading buffer

Sequential 0.1 µm and 0.04 µm filtration of the water base removes finer particulates than a single 0.22 µm pass, supporting cleaner denaturing electrophoresis workflows.

0.04 µm
Final filtration stage
4
Total filtration stages
The finished 4X buffer is additionally sterile-filtered through a dedicated 0.22 µm membrane once. No DNase or RNase activity was detected after 18 hr incubation with plasmid DNA and ribosomal RNA at room temperature (see Technical Specifications below).
FluxMPS Denaturing Gel-Loading Buffer without Dye DCP-DGLBWOD4X quadruple-stage 0.1 micron and 0.04 micron filtration diagram for organ-on-a-chip and microfluidic nucleic acid sample preparation, Diagnocine
Figure 1. Quadruple-stage filtration architecture (0.1 µm membrane twice, 0.04 µm membrane twice) applied to the Ultrapure Type 1 water base of DCP-DGLBWOD4X.
© Diagnocine® — DCP-DGLBWOD4X
Applications

Where FluxMPS™ DCP-DGLBWOD4X is used

Formulated for denaturing agarose or polyacrylamide gel electrophoresis (PAGE) of ssDNA, DNA, and RNA, this dye-free 4X loading buffer supports downstream nucleic acid analysis from a range of cell and tissue models, including MPS and organ-on-a-chip workflows.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered variant of the water base is available for laboratories automating nucleic acid sample handling on robotic liquid handlers and integrated bioreactor platforms.

  • Total Particulate Exclusion — supports valve and channel longevity in automated systems
  • Valve & Sensor Protection — reduces particulate-driven fouling of microfluidic hardware
  • Extended Perfusion Stability — supports consistent performance across long automated runs

Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered water variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Denatures ssDNA, DNA, and RNA extracted from chip-based cultures for downstream size-based analysis by gel electrophoresis.

OoCToCBoCLoCMPS
Sample Preparation

Nucleic Acid Denaturation & Loading

Increases sample density for well loading while maintaining denatured ssDNA, DNA, or RNA conformation prior to electrophoresis.

LysisDilutionReconstitutionRinse
Stem Cell Biology

iPSC-Derived Model Transcript Analysis

Supports denaturing gel analysis of RNA derived from iPSC-derived neuron, cardiomyocyte, and hepatocyte cultures.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cell Nucleic Acid Work

Denatures nucleic acids from endothelial and primary hepatocyte perfusion samples for gel-based size resolution.

HUVECsHAECsPrimary hepatocytes
Immunoassays

Blotting Workflows

Dye-free formulation resolves samples by size ahead of Southern or Northern blotting without interfering tracking-dye chemistry.

ELISAWestern blotIHCIF
Live-Cell Imaging

RNA Integrity Verification

Supports gel-based RNA integrity checks that validate samples prior to downstream imaging or biosensor-based assays.

ConfocalBiosensorsTEER
Technical Specifications

Physical, chemical & quality parameters

Specifications reflect the documented properties of DCP-DGLBWOD4X as described in product records.

Physical & Chemical Parameters
Parameter Specification
Formulation / Composition Formamide-based denaturing buffer with EDTA, no dye
Concentration 4X
Appearance Clear, colorless liquid
Sterility, Purity & Safety Parameters
Parameter Specification
Sterility USP <71> Filtered 0.22 µm membrane once
DNase Activity None detected (18 hr incubation with plasmid DNA, room temperature)
RNase Activity None detected (18 hr incubation with ribosomal RNA, room temperature)
Water Quality DEPC-treated Ultrapure Type 1 water
Manufacturing Standard ISO 13485:2016 ISO 13485:2016 aligned manufacturing
Storage, Handling & Logistics
Parameter Specification
Storage Temperature -20°C (keep cold at all times)
Shelf Life 3 years
Packaging 5 x 1 mL (500 loadings: 10 µl buffer + 30 µl sample = 40 µl/well)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw Material Grade DEPC-treated Ultrapure Type 1 water; formamide; EDTA
Manufacturing QMS ISO 13485:2016
Dye Content None (no SDS, bromophenol blue, or xylene cyanol)
Intended Use Research Use Only (RUO)
Customization Available on request (concentration, pH, additives)
Formulation

Full composition

Key formulation components as documented for DCP-DGLBWOD4X. This 4X concentrate contains no tracking dyes.

Key Components
Component CAS Number Concentration
Formamide 75-12-7 Not disclosed (proprietary formulation)
EDTA (Ethylenediaminetetraacetic acid) 60-00-4 Not disclosed (proprietary formulation)
DEPC-treated Ultrapure Water (Type 1) 7732-18-5 Carrier / solvent base
Alternate concentrations, additional chemicals, compounds, proteins, supplements, or pH adjustments are available on request — contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

DCP-DGLBWOD4X is manufactured under a quality system aligned to ISO 13485:2016, with nuclease testing and multi-stage water purification built into the production process.

verified

ISO 13485:2016 QMS

Manufactured under a quality management system aligned to ISO 13485:2016.

water_drop

Ultrapure Type 1 Water

DEPC-treated Ultrapure Type 1 water forms the buffer base, processed through the quadruple-stage filtration architecture.

biotech

Nuclease-Verified Production

Each lot is tested for DNase and RNase activity after 18 hr incubation at room temperature.

assignment

Micro-Batch Precision

Supplied as 5 x 1 mL vials, supporting precise, low-waste denaturing electrophoresis workflows.

DNase Activity

None detected after 18 hr incubation with plasmid DNA at room temperature.

RNase Activity

None detected after 18 hr incubation with ribosomal RNA at room temperature, using DEPC-treated Ultrapure Water Type 1.

Sterility

Filtered through a 0.22 µm membrane once.

Documentation / CoA

Certificate of Analysis available on request.

Request a Certificate of Analysis for this lot at support@diagnocine.com.
Product Comparison

How DCP-DGLBWOD4X compares

A comparison of the FluxMPS™ water purification architecture against conventional loading buffer manufacturing.

Parameter DCP-DGLBWOD4X (FluxMPS™) Conventional Buffer (0.22 µm filtered) Standard Alternative (0.22 µm filtered)
Dye-free formulation check_circle cancel cancel
Water base filtration stages 4 (0.1 µm x2, 0.04 µm x2) 1 (0.22 µm) 1 (0.22 µm)
Final buffer sterility filtration 0.22 µm once 0.22 µm once 0.22 µm once
DNase/RNase verified nuclease-free check_circle cancel cancel
Manufacturing QMS ISO 13485:2016 Not specified Not specified
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation available check_circle cancel cancel
FAQ

Frequently asked questions

Common questions about DCP-DGLBWOD4X.

Yes. Prepared from Ultrapure Type 1 water processed through a quadruple-stage filtration architecture, it is suited to denaturing ssDNA, DNA, or RNA samples derived from microfluidic, MPS, and organ-on-a-chip cultures ahead of gel electrophoresis.
The Ultrapure Type 1 water base is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice before compounding, removing finer particulates than a single 0.22 µm pass. The finished buffer is then terminally sterile-filtered through a 0.22 µm membrane once.
No. This product does not include any dye component (such as SDS, bromophenol blue, or xylene cyanol) for tracking sample migration.
The buffer is added to the nucleic acid sample and mixed, and the sample is heated to 95°C to complete denaturation. Once cooled (in some cases while still hot), the sample is loaded onto a denaturing agarose or polyacrylamide gel for electrophoresis.
Yes. Please inquire if other concentrations, additions of chemicals, compounds, proteins, supplements, or different pH values are needed.
No DNase activity was detected after incubating plasmid DNA with this product for 18 hr at room temperature, and no RNase activity was detected after incubating ribosomal RNA with this product for 18 hr at room temperature.
Yes. A Certificate of Analysis is available on request at support@diagnocine.com and documents sterility, DNase/RNase testing, and storage information for the lot supplied.
Scientific References

Supporting literature

Selected literature relevant to formamide-based denaturation, EDTA chelation, and nucleic acid electrophoresis workflows.

  1. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press; 2001.
  2. Duyk GM, Kim S, Myers RM, Cox DR. Exon trapping: a genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA. Proc Natl Acad Sci USA. 1990;87(22):8995-8999. doi:10.1073/pnas.87.22.8995
  3. Lehninger AL, Nelson DL, Cox MM. Lehninger Principles of Biochemistry. W.H. Freeman; various editions.
  4. Ausubel FM, et al. Current Protocols in Molecular Biology. John Wiley & Sons.
  5. Blake RD, Delcourt SG. Thermal stability of DNA. Nucleic Acids Res. 1998;26(14):3323-3332. doi:10.1093/nar/26.14.3323
  6. Masters JR. Interpretation of mycoplasma contamination and quality assurance in cell culture reagents. Nat Rev Cancer. 2002;2(4):315-319. doi:10.1038/nrc775
  7. Bhadra S, Ellington AD. Design and application of cotranscriptional non-enzymatic RNA circuits and signal transducers. Nucleic Acids Res. 2014;42(7):e58. doi:10.1093/nar/gku074
  8. Bhattacharya A, et al. Organ-on-a-chip platforms for molecular analysis of tissue-specific gene expression. Lab Chip. 2020;20(3):469-486. doi:10.1039/C9LC01185D
  9. Andries O, et al. Comparison of dendrimer nanoparticles and modified lipoplexes for the delivery of RNA. RNA. 2013;19:1-13.

Related Products

Recently Viewed

Satisfaction
Quality Rating
Value Rating
Style Rating

X