Denaturing Gel-Loading Buffer with Dye, (4X)

FluxMPS™ Denaturing Gel-Loading Buffer with Dye, (4X)

A formamide-based, EDTA-chelated denaturing gel-loading buffer formulated as a 4X concentrate for denaturing agarose and polyacrylamide gel electrophoresis (PAGE) of ssDNA, DNA, and RNA. Manufactured with DEPC-treated Ultrapure Type 1 water and finished through a quadruple-stage 0.1 µm / 0.04 µm filtration process, then verified DNase- and RNase-free before release — keeping your denaturing electrophoresis workflow free of contaminating nuclease activity.

• Formamide-based denaturing agent resolves secondary structure in ssDNA, DNA, and RNA
• EDTA chelation of Ca²⁺ and Mg²⁺ protects nucleic acids from metal-dependent nuclease activity
• Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice
• DEPC-treated Ultrapure Type 1 water supports RNase-free performance
• Built-in bromophenol blue and xylene cyanol dyes for real-time migration tracking
• DNase and RNase activity verified by 18-hour challenge testing
• ISO Class 5 aseptic fill under an ISO 13485:2016 quality management system
• Custom concentrations, additives, and pH available on request

Engineered where standard gel-loading buffers fall short

Conventional 0.22 µm-filtered loading buffers can carry subvisible particulate and residual nuclease activity into denaturing electrophoresis workflows, distorting migration patterns and risking degradation of sensitive ssDNA, RNA, or nicked-plasmid samples. FluxMPS™ formulation and filtration controls are built to eliminate those failure modes at the source.

Quadruple-stage filtration system

Every lot of this denaturing gel-loading buffer passes through four sequential filtration stages — 0.1 µm membrane filtration twice, followed by 0.04 µm membrane filtration twice — before DEPC-treated Ultrapure Type 1 water is used to formulate the final 4X concentrate.

1. 1

   0.1 µm Pre-filtration I

   Removes large particulate and aggregates, extending the working life of downstream filters.

2. 2

   0.04 µm Pre-filtration II

   Retains fine particulates and bioburden that pass an initial 0.1 µm stage, including organisms in the mycoplasma size range.

3. 3

   0.1 µm Sterile-filtration I

   A second 0.1 µm pass provides redundancy ahead of final polishing.

4. 4

   0.04 µm Sterile-filtration II — Final Polish

   A second 0.04 µm pass delivers the final polish under an ISO Class 5 aseptic fill.

Built for denaturing nucleic acid electrophoresis

Formamide denatures secondary structure in ssDNA, DNA, and RNA so that samples migrate through a denaturing gel by size rather than shape — letting researchers resolve nicked-plasmid ssDNA, individual RNA species, and other structured nucleic acids with confidence.

Automated Bioreactors & Robotics

Labs running automated liquid handling for high-throughput gel-loading workflows can request an optional 0.01 µm (10 nm) ultra-filtered variant to further protect valves, tips, and sensors in automated sample-prep robotics.

• Total Particulate Exclusion — sub-0.04 µm polishing minimizes residual particulate in automated dispensing lines
• Valve & Sensor Protection — reduces particulate load on precision liquid-handling components
• Extended Perfusion Stability — supports consistent performance across long automated run sequences

Inquiry Required: the 0.01 µm ultra-filtered grade is available on request — contact support@diagnocine.com.

Formulation, purity, and handling data

Specification values shown below are as stated for this product; parameters not published for this formulation are omitted rather than estimated.

Full composition

Formamide and EDTA provide the denaturing and chelating chemistry of this 4X concentrate; bromophenol blue and xylene cyanol serve as built-in tracking dyes.

Manufacturing & compliance

Every lot is manufactured and tested under a documented quality system before release.

How DCP-DGLBWD4X compares

A side-by-side look at filtration, purity verification, and formulation transparency versus conventional gel-loading buffers.

Frequently asked questions

Common questions about DCP-DGLBWD4X denaturing gel-loading buffer.

Supporting literature

Curated references on formamide-based denaturation, EDTA chelation, and nucleic acid electrophoresis relevant to this buffer's use.

1. Rio DC. Denaturation and electrophoresis of RNA with formamide/formaldehyde. doi:10.1101/pdb.prot5445
2. Goda SK, Minton NP. Rapid analysis of nucleic acids via formamide-based denaturing gel systems. Nucleic Acids Res. doi:10.1093/nar/23.16.3357
3. Chelation of divalent metal ions by EDTA and its role in nuclease inhibition during nucleic acid handling. doi:10.1016/0003-2697(87)90021-2
4. Use of bromophenol blue and xylene cyanol as migration tracking dyes in gel electrophoresis. doi:10.1101/pdb.rec12299
5. DEPC treatment for preparation of RNase-free reagents and water. doi:10.1006/abio.1994.1112
6. Control of nuclease and particulate contamination in molecular biology reagent manufacturing. doi:10.1016/j.ab.2015.01.010
7. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual — denaturing gel electrophoresis of nucleic acids. doi:10.1101/pdb.top083055
8. Nucleic acid sample preparation workflows for microfluidic and organ-on-a-chip downstream analysis. doi:10.1039/C7LC00312A