DNA Polymerase β,Functional, Rat

DNA Polymerase β,Functional, Rat

Cat. No. FNK-10-101
Store at -80°C
Size 20µg


Description 

DNA polymeraseβ is a distributive polymerase involved in base excision repair which repairs damaged DNA by excising modified bases (oxidized, methylated, deaminated etc.) (Ref. 1). This product is a highly purified full-length rat DNA polymerase β overproduced in E. coli with high enzymatic activity without any tags attached (Ref. 2). The enzyme has a molecular mass of 38 kDa (Fig.1). The amino acid sequence of the rat enzyme has 86% identity to the human homolog.

Properties of the product

Enzyme activity 90 unit/ul (1unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37℃ in 60 min.)
PurityOver 95% pure by SDS-PAGE analysis
Form1.3 mg/ml in 50mM Tris-HCl pH7.6, 0.3M KCl, 0.1mM EDTA, 1mM DTT, 20% Glycerol

Fig.1 SDS-PAGE analysis of DNA polymeraseβ

M Molecular weight markers (from top: 250, 150, 100, 75, 50, 37, 25, 20 kDa) ,Lane 1 DNA polymeraseβ (rat) 

Applications

• For studies on the mechanisms of base-excision repair of DNA damage
• As a positive control of western blotting with anti-DNA polymeraseβ antibody 

References

1. Friedberg EC, et al. DNA Repair and Mutagenesis 2nd ed., ASM Press (2006)
2. Date T. et al. Biochemistry 27: 2983 (1988)