DNA Loading Buffer [6X]
Cat No.: DCP-DNALB6X
Size: 5 x 1 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
DNA Loading Buffer facilitates the loading process by increasing the density of the sample and providing visual tracking. This buffer has sucrose, which has a higher density than the running buffer used for gel electrophoresis. When added to the DNA sample, it increases the overall density of the mixture relative to the buffer. The increased density helps the DNA sample sink to the gel wells' bottom during loading rather than diffusing into the surrounding running buffer. By ensuring the DNA sample remains concentrated within the wells, sucrose helps prevent sample loss or diffusion, which could lead to poor resolution or distorted bands. Sucrose is less viscous and sticky than glycerol, making it easier to pipette and handle.
* Please note that this buffer contains bromophenol blue and xylene cyanol.
Advantages:
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Aids in loading DNA samples into gel wells.
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Provides visual tracking of DNA migration.
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Prevents sample loss or diffusion.
Composition:
| Ingredients |
Concentration |
| Sucrose |
40% |
| Bromophenol blue |
0.25% |
| Xylene cyanol |
0.25% |
| Tris-HCl |
10 mM |
| EDTA |
60mM |
Quality Control:
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Appearance: blue-purple or violet color.
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pH: 7.4
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -20 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
