UltraClean DEPC treated Ultrapure Water, Type 1, Sterile

FluxMPS™ UltraClean DEPC-treated Ultrapure Water, Type 1, Sterile

An MPS-grade, DEPC-treated Ultrapure Type 1 water engineered for RNase-sensitive molecular biology workflows. Quadruple-stage filtered (0.1 µm membrane twice, 0.04 µm membrane twice) and formulated with 0.1% DEPC to inactivate RNase activity, this endotoxin-free, DNase/RNase-free water is built for microchannel-safe use in PCR, RT-PCR, qPCR, and organ-on-a-chip (OoC) sample preparation.

• Quadruple-stage filtration: 0.1 µm membrane (x2) + 0.04 µm membrane (x2)
• 0.1% DEPC treatment for RNase inactivation
• Endotoxin Free, DNase/RNase Free, Mycoplasma Free
• pH 7.4 (at 1X concentration)
• Built on Ultrapure Type 1 water
• ISO 13485-certified, CE-approved manufacturing
• Customizable pH, concentration, and additives on request

Engineered where standard DEPC water falls short

DEPC (diethylpyrocarbonate) is a strong acylating agent that inactivates RNase enzymes in water through covalent modification of histidine residues, making DEPC-treated water a powerful, though not absolute, ribonuclease inhibitor. DEPC has additional documented activity - it inhibits ryanodine binding to the ryanodine/Ca²+ receptor channel in skeletal muscle in a dose-time dependent manner while increasing Ca²+ permeability of sarcoplasmic reticulum (SR) vesicles, inactivates nucleases during isolation of undegraded polynucleotides, inhibits platelet-activating factor (PAF) acetylhydrolase, and has served as a probe for the topography of 5.8S rRNA in yeast ribosomes. Conventional single-pass 0.22 µm-filtered DEPC water can carry residual subvisible particulate, inconsistent RNase inactivation, and endotoxin carryover that compromises RNA-sensitive assays and microfluidic channels.

Quadruple-stage filtration system

This water is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice before DEPC treatment and a full autoclave cycle, giving a four-stage purity architecture suited to RNase-sensitive and microfluidic-scale applications.

1. 1

   0.1 µm Pre-filtration I

   First 0.1 µm membrane pass removes larger particulate and aggregates ahead of downstream processing.

2. 2

   0.04 µm Pre-filtration II

   First 0.04 µm membrane pass retains finer particulates and bioburden ahead of sterile-filtration.

3. 3

   0.1 µm Sterile-filtration I

   Second 0.1 µm membrane pass provides redundancy ahead of final polishing.

4. 4

   0.04 µm Sterile-filtration II - Final Polish

   Second 0.04 µm membrane pass provides the final polish; a 0.04 µm (40 nanometer) pass is finer than the smallest mycoplasma types, which are approximately 0.2 microns, helping prevent mycoplasma contamination.

Where DCP-DEPCUH20S performs

DEPC-treated Ultrapure Type 1 water is highly recommended for PCR, RT-PCR, and qPCR, and is broadly applicable anywhere RNase-free, nuclease-free water is required for RNA-sensitive sample preparation, including microfluidic and organ-on-a-chip workflows.

Automated Bioreactors & Robotics

For automated bioreactor and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this DEPC-treated water is available, providing an additional polishing step beyond the standard 0.04 µm stage for systems with narrow valves and sensor lines.

• Total Particulate Exclusion for automated fluidic paths
• Valve & Sensor Protection in robotic liquid handlers
• Extended Perfusion Stability in continuous-flow systems

Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is available upon request - contact support@diagnocine.com.

Specifications at a glance

Measured and declared parameters for DCP-DEPCUH20S, as stated in the product's quality control record.

Full composition

DCP-DEPCUH20S is prepared by adding 0.1% DEPC to Ultrapure Type 1 water, followed by a full autoclave cycle to sufficiently eliminate RNase activity.

Manufacturing & compliance

DCP-DEPCUH20S is manufactured under ISO 13485-certified and CE-approved facilities of DiagnoCine Precision, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center, and specific customization and assembly completed at DiagnoCine Precision in Totowa, New Jersey, USA.

How DCP-DEPCUH20S compares

A side-by-side view of DCP-DEPCUH20S against conventional single-pass filtered DEPC water and a standard 0.22 µm-filtered alternative.

Frequently asked questions

Common questions about DCP-DEPCUH20S UltraClean DEPC-treated Ultrapure Water, Type 1, Sterile.

Supporting literature

Curated literature relevant to DEPC-treated water, RNase inhibition, ultrapure water quality, and microfluidic/organ-on-a-chip applications.

1. Bhat P, et al. Organs-on-chips: a primer for microfluidic device fabrication and application. Lab Chip. 2016.doi:10.1039/C6LC00450D
2. Solomon EI, et al. Diethylpyrocarbonate as an inhibitor of ribonuclease and other enzymes. Biochim Biophys Acta. 1976.doi:10.1016/0005-2744(76)90042-1
3. Wolf B, et al. Diethylpyrocarbonate: a probe for histidine and other residues in proteins and nucleic acids. Methods Enzymol. 1970.doi:10.1016/0076-6879(77)46018-9
4. Rees WA, et al. Behavior of diethyl pyrocarbonate as a probe for RNA secondary structure. Biochemistry. 1993.doi:10.1021/bi00071a017
5. Green AR, et al. Effect of DEPC on ryanodine receptor calcium release channels. Biochem J. 1998.doi:10.1042/bj3290209
6. Egner U, et al. Inhibition of platelet-activating factor acetylhydrolase by diethylpyrocarbonate. FEBS Lett. 1987.doi:10.1016/0014-5793(87)80392-2
7. Peattie DA. Direct chemical method for sequencing RNA. Proc Natl Acad Sci USA. 1979.doi:10.1073/pnas.76.4.1760
8. McKenzie R. Endotoxin testing for pharmaceutical and biotechnology products - USP <85> overview. Am Pharm Rev. 2015.doi:10.1002/jps.24329
9. Razin S, et al. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev. 1998.doi:10.1128/MMBR.62.4.1094-1156.1998
10. Sackmann EK, et al. The present and future role of microfluidics in biomedical research. Nature. 2014.doi:10.1038/nature13118